Effect of Irradiation Dose and Storage Time on the EPR Signal Intensities of Gamma Irradiated Sulfapyridine

Abstract

Abstract:The interest in sterilizing the medicine using gamma energy is increasing. However, radiation may cause molecular degradation in the sample and thus may lead to the formation of free radicals due to high energy. This study aims to determine the radiation sensitivity of sulfapyridine (SU) irradiated with a predetermined dose using EPR spectroscopy. In the EPR spectra recorded at room temperature prior to radiation process, no EPR signal was observed. However, after the irradiation with gamma rays, EPR signal intensity increased significantly based on the irradiation dose.Mathematical functions and correlation coefficients were calculated determining the relation between the increasing radiation doses on the signal intensities of the sample. The stability of the free radicals created after the irradiation in time was investigated by recording the spectra of the sample irradiated with a dose value of 15 kGy in certain intervals. The results were found to be in good agreement with the existing literature data.

Işınlama Dozunun ve Depolama Süresinin Gama ile Işınlanmış Sülfapridinin Sinyal Şiddeti Üzerine Etkisi

Abstract

İlaçların gama enerjisi kullanılarak sterilizasyonuna olan ilgi artmaktadır. Ancak radyasyon yüksek enerjisinden dolayı örnekte moleküler yıkımlara sebep olabilmekte ve bu durum örnekte serbest radikaller oluşturabilmektedir. Bu çalışmanın amacı; belirli dozlarda ışınlanan sülfapridinin (SU) radyasyon duyarlılığını EPR spektroskopisi kullanarak belirlemektir. Işınlanmamış örneğin elde edilen EPR spektrumlarında hiç bir EPR sinyali gözlenmezken; gama ışınları ile ışınlanmanın ardından, artan ışınlama dozuna bağlı olarak belirgin EPR sinyalleri elde edildi. Örneğin artan radyasyon dozuna bağlı sinyal şiddetindeki artışı tanımlayan matematiksel denklemler ve korelasyon katsayıları hesaplandı. 15 kGy ışınlanmış örneğin belirli aralıklarla spektrumları kaydedilerek ışınlama işleminden sonra oluşan serbest radikallerin kararlılığı incelendi. Sonuçların mevcut literatür verileri ile uyum içinde olduğu tespit edildi.

Keywords

• Elektron Paramanyetik Rezonans (EPR),Radyasyon Duyarlılığı,Antibiyotik

References

1. Gopal, N.G.S., 1978, Radiation sterilization of pharmaceuticals and polymers. Radiation Physics and Chemistry, 12, 35-50.
2. Diehl, J.F., 2002, Food irradiation—past, present and future. Radiation Physics and Chemistry. 63, 211–215.
3. Bhat, R., Sridhar, K.R. 2011, Influence of ionizing radiation and conventional food processing treatments on the status of free radicals in lotus seeds: an EPR study. Journal of Food Composition and Analysis, 24, 563-567.
4. Ambroz, H.M., Kornacka, E.M., Marciniec, B., Przybytniak, G., 2002, Radical decay in irradiated drugs: Flutamide, ifosamide and aminglutethimide. Journal of Radioanalytical and Nuclear Chemistry, 254, 293-298.
5. Basly, J.P., Basly, I., Bernard, M., 1998a, Electron spin resonance detection of radiosterilization of pharmaceuticals: application to four nitrofurans. The Analyst, 123, 1753-1756.
6. Basly, J.P., Bernard, M., 1997, Radiosterilization dosimetry by ESR spectroscopy: ritodrine hydrochloride and comparison with other sympathomimetics. International Journal of Pharmaceutics, 149, 85-91.
7. Delincee, H., 2002. Analytical methods to identify irradiated food—a review.Radiation Physics and Chemistry, 63, 455–458.
8. Murrieta, H., Munoz, E., Adem, E., Burillo, G., Vazquez, M., Cabrera, E., 1996. Effect of irradiation dose, storage time and temperature on the ESR signal in irradiated oat, corn and wheat.Applied Radiation and Isotopes, 47, 1657-1661.

9. Ambroz, H.B., Kornacka, E.M., Marciniec, B., Ogrodowczyk, M., Przybytniak, G.K., 2000, EPR study of free radicals in some drugs γ-irradiated in the solid state. Radiation Physics and Chemistry, 58, 357-366.
10. Rajendiran, N., Siva, S., Saravanan,J., 2013, Inclusion complexation of sulfapyridine with a- and b-cyclodextrins:Spectral and molecular modeling study. Journal of Molecular Structure, 1054-1055, 215-222.
11. Challis, J.K., Carlson, J.C., Friesen, K. J., Hanson, M.K., Wong, C.S.,2013, Aquatic photochemistry of the sulfonamide antibiotic sulfapyridine. Journal of Photochemistry and Photobiology A: Chemistry, 262, 14-21.
12. Damian, G., 2003, EPR investigation of γ-irradiated anti-emetic drugs. Talanta, 60, 923-927
13. Polat, M, Korkmaz, M (2006), Journal of Pharmaceutical and Biomedical Analysis, 40: 882-888.
14. Basly, J.P., Basly, I., Bernard, M., 1998b, Electron spin resonance identification of irradiated ascorbic acid: Dosimetry and influence of powder fineness. AnalyticaChimicaActa, 372, 373-378.
15. Marralle, M., Longo, A., Panzeca, S., Gallo, S., Principato, F., Tomarchio, E., Parlato, A., Buttafava, A., Dondi, D., Zeffiro, A., 2014, ESR response of phenol compounds for dosimetry of gamma photon beams. Nuclear Instruments and Methods in Physics Research B, 339,15-19.
16. Yurus, S., Ozbey, T., Korkmaz, M., 2004, ESR investigation of gamma irradiated sulbactam sodium. Journal of Pharmaceuitcal and Biomedical Analysis, 35, 971-978.
17. Tuner, H., Korkmaz, M., 2007, Radiostability of butylated hydroxytoluene (BHT): an ESR study. Beam Interactions with Materials and Atoms, 258, 388-394.
18. Basly, J.P., Longy, I., Bernard, M., 1998c, Radiosterilization dosimetry by electron-spin resonance spectroscopy: Cefotetan. AnalyticaChimicaActa, 359, 107-113.