Development of Potentiometric Sensor for Determination of Isoprenaline in Pharmaceutical Drug

Abstract

In this study, a potentiometric ion-selective electrode (ISE) was developed for the determination of isoprenaline, a pharmaceutical active substance. For this purpose, initially, IP-PM, IP-TCA, IP-TPB, IP-REY, IP-PTA ion pairs were synthesized. The synthesized ion pairs were used as the ionophore in the structure of the electrode membrane. In order to ensure membrane optimization, PVC membrane ion selective electrodes were produced in various compositions using the synthesized ion pair and the potentiometric performance characteristics of these electrodes were investigated. It was determined that the best potentiometric performance characteristics were obtained with the PVC membrane electrode in the composition of 3.0% isoprenaline-tetrafenylborate ion pair, %64.0 nitrophenyloctylether, 32.0% polyvinylchloride and %1.0 Tetrakis (4-chlorophenyl) boron potassium compound. The linear operating range of this electrode is 5.0x 10^-6 M - 1.0x 10^-1 M and the slope at the 10-fold concentration change is 45.3 mV; determination limit, 5.0x10^-6 M, pH working range, 2.6-3.6 and 5.7-7.9; response time < 5s. The electrode exhibited a reproducible potentiometric response. Isoprenaline content was determined in drug by using the isoprenaline selective electrode. 

Keywords

• Isoprenaline,potentiometric sensor

Project Number

FYL-2018-7184

Farmasötik İlaçta İzoprenalin Tayini İçin Potansiyometrik Sensör Geliştirilmesi

Abstract

Bu çalışmada, farmasötik etken madde olan izoprenalinin tayini için potansiyometrik iyon seçici bir elektrot (İSE) geliştirildi. Bu amaçla, ilk olarak, İP-FM, İP-TSA, İP-TPB, İP-REY, İP-FTA iyon çiftleri sentezlendi. Sentezlenen iyon çiftleri elektrot membranının yapısında iyonofor olarak kullanıldı. Membran optimizasyonunu sağlamak için sentezlenen iyon çiftleri kullanılarak çeşitli bileşimlerde PVC membran elektrotlar üretildi. Bu elektrotların potansiyometrik performans özellikleri araştırıldı. En iyi potansiyometrik performans özelliklerinin % 3.0 İzoprenalin-Tetrafenilborat iyon çifti, % 64 Nitrofeniloktileter (NPOE), % 32.0 Polivinilklorür (PVC), % 1 Potasyum tetrakis (4-klorofenil) borat (KTpClPB) bileşimine sahip membran kullanılarak elde edildi. Bu elektrodun doğrusal çalışma aralığının 5.0x 10^-6 M-1.0x 10^-1 M ve 10 katlık konsantrasyon değişimindeki eğimi, 45,3 mV; tayin limiti, 5.0x10^-6 M; pH çalışma aralığı 2.6-3.6 ve 5.7-7.9; cevap zamanı < 5 sn olarak belirlendi. Elektrot oldukça tekrarlanabilir bir potansiyometrik cevap sergilemiştir. İzoprenalin içeriği, izoprenalin seçici elektrot kullanılarak ilaçta tayin edildi.  

Keywords

• İzoprenalin,Potansiyometrik sensör

Supporting Institution

van yüzüncü yıl üniversitesi bilimsel araştırma projesi başkanlığı

Project Number

FYL-2018-7184

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