The Effect of Iloprost on Nitric Oxide, Asymmetric Dimethyl Arginine and Serotonin in the Treatment of Peripheral Vascular Disease

Abstract

Aim: In this study, it is aimed to investigate the efficacy of iloprost use in peripheral artery disease patients on the parameters involved in endothelial functions such as circulating acetyl dimethyl arginine (ADMA), serotonin and nitric oxide (NO). Patients and Methods: 30 patients (19 male, 11 female, age interval 60.7 ± 13.7) who were followed-up with the diagnosis of Fontaine III-IV disease and decided to receive a medical treatment were included in the study. Iloprost infusion was initiated as a 16-hour intravenous infusion at the dose of 0.5-1.5 ng / kg / min from the forearm veins and was given for 7 days. Blood was taken for ADMA, Serotonin and NO results before and after the treatment (8th day). Total nitrite (nitrite + nitrate) concentration was measured by the modified cadmium reduction method, ADMA and serotonin levels were measured by High Performance Liquid Chromotography (HPLC) method. Results: ADMA value before and after the iloprost treatment was decreased after the treatment and while this was statistically significant (p=0.001), the changes in serotonin (p=0.82) and NO (P= 0.16) values were found statistically insignificant. Conclisions: Peripheral arterial disease may be a parameter that can be taken into account in the evaluation of treatment for peripheral arterial disease and ADMA, which is considered to be an endothelial dysfunction indicator after iloprost treatment.

Keywords

• Iloprost
• Nitric Oxide
• ADMA
• Serotonin

Periferik Vasküler Hastalığın Tedavisinde İloprost’tun Nitrik Oksit, Asimetrik Dimetilarjinin ve Serotonin’e Etkisi

Abstract

Özet Amaç: Bu çalışmada periferik arter hastalarında iloprost kullanılımının dolaşımdaki asetil dimetil arjinin (ADMA), Serotonin ve Nitrik Oksit (NO) gibi endotelyal fonksiyonlarda görev alan parametreler üzerindeki etkinliğinin araştırılması hedeflenmiştir. Materyal Metod : Çalışmaya Fontaine III-IV hastalığı tanısıyla takip edilip medikal tedavi kararı verilen 30 olgu (19’u erkek, 11’i kadın, yaş aralığı ise 60.7 ± 13.7 ) alındı. Hastalara iloprost infüzyonu ön kol venlerinden 0.5-1.5 ng/kg/dk dozunda 16 saatlik intravenöz infüzyon şeklinde başlandı ve 7 gün verildi. Tedavi öncesi ve sonrası ( 8. günde) ADMA, Serotonin ve NO sonuçları için kan alındı. Total nitrit (nitrit + nitrat) konsantrasyonu modifiye kadmiyum redüksiyon metodu ile, ADMA ve serotonin düzeyleri High Performance Liquid Chromotography (HPLC) yöntemiyle ölçüldü. Bulgular: İloprost tedavisi öncesi ve sonrası ADMA değeri tedavi sonrası düşmüş olup bu istatistiksel olarak anlamlı iken (p=0.001), Serotonin (p=0.82) ve NO (P= 0.16) değerlerindeki degişiklikler istatistiksel olarak anlamsız bulunmuştur. Sonuç: Periferal arteryel hastalıklarda iloprost tedavisi sonrası endotelyal disfonksiyon göstergesi olarak kabul edilen ADMA’nın azaldığını ve periferik arteryel hastalarında kullanılan tedavinin değerlendirilmesinde dikkate alınabilinecek bir parametre olabilir .

Keywords

• İloprost
• Nitrik Oksit
• ADMA
• Serotonin

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