Thiacloprid ve D-Tubokurarin’in Rana ridibunda Gastrokinemius Kası Üzerine Toksik Etkileri III: Oksidatif Potansiyel

Öz

Bu çalışmada neonikotinoid bir insektisit olan thiacloprid ve antagonisti d-tubokurarin’in kurbağa gastrokinemius kasında, tiyobarbitürik asit reaktif madde düzeyleri ve katalaz enzim aktivitesi üzerine etkileri araştırılmıştır. Deneylerde 35 adet kurbağa kas preparatı kullanılmıştır. Gastrokinemius kası izole edildikten sonra, 120 dakika boyunca 250, 25, 2,5 ve 0,25 mg/L olmak üzere thiacloprid’in 4 farklı konsantrasyonuna maruz bırakılmıştır. Ayrıca, izole kaslar 2,5 mg/L thiacloprid ile 80 mg/L d-tubokurarin karışımı ve 0,25 mg/L thiacloprid ile 8 mg/L d-tubokurarin karışımı ile muamele edilmiştir. Kontrol grubundaki kas dokuları ise, 120 dakika boyunca Ringer çözeltisi içerisinde bekletilmiştir. Her bir konsantrasyon grubu eşit sayıda preparat ile çalışılmıştır (n=5).Yapılan incelemeler sonucunda 250, 25, 2,5 ve 0,25 mg/L thiacloprid, 0,25 mg/L thiacloprid ile 8 mg/L d-tubokurarin karışımının çizgili kas dokusunda kontrol grubuna göre, tiyobarbitürik asit reaktif madde düzeylerini arttırdığı gözlenmiştir (P<0,05). 250 (P<0,001) ve 25 mg/L thiacloprid (P<0,05), kas dokuda katalaz enzim aktivitesini azaltmıştır. Bu çalışma, thiacloprid ve d-tubokurarin’in çizgili kaslarda oksidatif stres oluşturma potansiyelinin, hedef olmayan organizmalara olası etkilerinin ve çevresel risklerinin değerlendirilmesinde önemli bilgiler sunmaktadır. 

Anahtar Kelimeler

• : Thiacloprid,d-tubokurarin,kurbağa,gastrokinemius

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