Thiacloprid ve D-Tubokurarin’in Rana ridibunda Gastrokinemius Kası Üzerine Toksik Etkileri III: Oksidatif Potansiyel

Öz

Bu çalışmada neonikotinoid bir insektisit olan thiacloprid ve antagonisti d-tubokurarin’in kurbağa gastrokinemius kasında, tiyobarbitürik asit reaktif madde düzeyleri ve katalaz enzim aktivitesi üzerine etkileri araştırılmıştır. Deneylerde 35 adet kurbağa kas preparatı kullanılmıştır. Gastrokinemius kası izole edildikten sonra, 120 dakika boyunca 250, 25, 2,5 ve 0,25 mg/L olmak üzere thiacloprid’in 4 farklı konsantrasyonuna maruz bırakılmıştır. Ayrıca, izole kaslar 2,5 mg/L thiacloprid ile 80 mg/L d-tubokurarin karışımı ve 0,25 mg/L thiacloprid ile 8 mg/L d-tubokurarin karışımı ile muamele edilmiştir. Kontrol grubundaki kas dokuları ise, 120 dakika boyunca Ringer çözeltisi içerisinde bekletilmiştir. Her bir konsantrasyon grubu eşit sayıda preparat ile çalışılmıştır (n=5).Yapılan incelemeler sonucunda 250, 25, 2,5 ve 0,25 mg/L thiacloprid, 0,25 mg/L thiacloprid ile 8 mg/L d-tubokurarin karışımının çizgili kas dokusunda kontrol grubuna göre, tiyobarbitürik asit reaktif madde düzeylerini arttırdığı gözlenmiştir (P<0,05). 250 (P<0,001) ve 25 mg/L thiacloprid (P<0,05), kas dokuda katalaz enzim aktivitesini azaltmıştır. Bu çalışma, thiacloprid ve d-tubokurarin’in çizgili kaslarda oksidatif stres oluşturma potansiyelinin, hedef olmayan organizmalara olası etkilerinin ve çevresel risklerinin değerlendirilmesinde önemli bilgiler sunmaktadır. 

Anahtar Kelimeler

• : Thiacloprid,d-tubokurarin,kurbağa,gastrokinemius

Kaynakça

1. Aebi, H. 1984. Catalase in vitro. Methods Enzymol, 105,121-126.
2. Aruoma, O.I. 1998. Free Radicals, Oxidative Stress, and Antioxidants in Human Health and Disease. Journal of the American Oil Chemists’ Society, 75,199-212.
3. Aydın, B. 2011. Effects of Thiacloprid, Deltamethrin and Their Combination on Oxidative Stress in Lymphoid Organs, Polymorphonuclear Leukocytes and Plasma of Rats. Pesticide Biochemistry and Physiology, 100,165-171.
4. Ballı, E., Yalın, S., Mazmancı, B., Mazmancı, M.A., Söğüt, F., Eroğlu, P., Yetkin, D., Korkutan, S., Çömelekoğlu, Ü. 2014. Deltametrinin Oluşturduğu Periferik Sinir Hasarı Üzerine E Vitaminin Etkisinin Araştırılması. Mersin Üniversitesi Sağlık Bilimleri Dergisi, 7(1), 17-23.
5. Banerjee, B.D., Seth, V., Bhattacharya, A., Pahsa, S.T., Chakraborty, A.K. 1999. Biochemical Effects of Some Pesticides on Lipid Peroxidation and Free-Radical Scavengers. Toxicology
6. Cheeseman, K.H. 1993. Mechanisms and Effects of Lipid Peroxidation. Molecular Aspects of Medicine, 14(3), 191-197.
7. Clasen, B., Leitemperger, J., Murussi, C., Pretto, A., Menezes, C., Dalabona, F., Marchezan, E., Adaime, M.B., Zanella, R., Loro, V.L. 2014. Carbofuran Promotes Biochemical Changes in Carp Exposed to Rice Feld and Laboratory Conditions. Ecotoxicology and Environmental Safety, 101, 77-82.
8. Düzgüner, V., Erdoğan, S. 2010. Acute Oxidant and Inflammatory Effects of Imidacloprid on the Mammalian Central Nervous System and Liver in Rats. Pesticide Biochemistry and Physiology, 97(1),13-18.

9. Düzgüner, V., Erdoğan, S. 2012. Chronic Exposure to Imidacloprid Induces Inflammation and Oxidative Stress in the Liver and Central Nervous System of Rats. Pesticide Biochemistry and Physiology, 104(1), 58-64.
10. El-Gendy, K.S., Aly, N.M., Mahmoud, F.H., Kenawy, A., El-Sebae, A.K. 2010. The Role of Vitamin C as Antioxidant in Protection of Oxidative Stress Induced by Imidacloprid. Food and Chemical Toxicology, 48(1), 215-221.
11. Grisham, M.B., Jourd'heuil, D., Wink, D.A. 1999. Physiological Chemistry of Nitric Oxide and Its Metabolites: Implications in Inflammation. American Journal of Physiology, 276(2), 315-321.
12. John, S., Kale, M., Rathore, N., Bhatnagar, D. 2001. Protective Effect of Vitamin E in Dimethoate and Malathion Induced Oxidative Stress in Rat Erythrocytes. The Journal of Nutritional Biochemistry, 12, 500-504.
13. Kanbur, M., Liman, B.C., Eraslan, G., Altınordulu, S. 2008. Effects of Cypermethrin, Propetamphos, and Combination Involving Cypermethrin and Propetamphos on Lipid Peroxidation in Mice. Environmental Toxicology, 23(4), 473-479.
14. Keramati, V., Jamili, S., Ramin, M. 2010. Effect of Diazinon on Catalase Antioxidant Enzyme Activity in Liver Tissue of Rutilus rutilus. Journal of Fisheries and Aquatic Science, 5, 368-376.
15. Klaunig, J.E. 1991. Alterations in Intracellular Communication During the Stage of Promotion. Experimental Biology and Medicine, 198(2), 688-692.
16. Kocaman, A.Y., Topaktaş, M. 2007. In Vitro Evaluation of the Genotoxicity of Acetamiprid in Human Peripheral Blood Lymphocytes. Environmental and Molecular Mutagenesis, 48, 483-490.
17. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1961. Protein Measurement with the Folin Phenol Reagent. The Journal of Biological Chemistry, 193, 265-275.
18. Moraes, B.S., Loro, V.L., Pretto, A., Fonseca, M.B., Menezes, C., Marchesan, E., Reimche, G.B., Avila, L.A. 2009. Toxicological and Metabolic Parameters of the Teleost fish (Leporinus obtusidens) in Response to Commercial Herbicides Containing Clomazone and Propanil, Pesticide Biochemistry and Physiology, 95, 57-62.
19. Mullins, J.W. 1993. Imidacloprid: A New Nitroguanidine Insecticide. American Chemical Society Symposium Series, 524, 183-198.
20. Nauen, R. 1995. Behaviour Modifying Effects of Low Systemic Concentrations of Imidacloprid on Myzus persicae with Special Reference to An Antifeeding Response. Pesticide Science, 44(2), 145-153.
21. Ohkava, H., Ohisini, N., Tagi, K. 1979. Assay For Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction. Analytical Chemistry, 95, 351-358.
22. Özkol, H., Tülüce, Y., Çelik, İ., Işık, İ. 2016. Omethoate Modulates Some Oxidant/Antioxidant Parameters in Frogs (Rana ridibunda Pallas). Toxicology and Industrial Health, 28(4), 320-326.
23. Rehman, H., Ali, M., Atıf, F., Kaur, M., Bhatia, K., Raisuddin, S. 2006. The Modulatory Effect of Deltamethrin on Antioxidants in Mice. Clinica Chimica Acta, 369, 61-65.
24. Rosner, B. 1995. Fundamentals of Biostatistics. Fourth ed. Duxbury Press, Boston, pp.299-344.
25. Scassellati, S.G., Moretti, M., Villarini, M., Angeli, G., Pasquini, R., Monarca, S., Scarselli, R., Crea, M.G., Lonardis, C. 1994. An Evaluation of Toxic and Genotoxic Risk From Work Related Exposure to Chemical Compounds. Prevenzione Oggi, 6, 125-138.
26. Singh, M., Sandhir, R., Kiran, R. 2006. Erythrocyte Antioxidant Enzymes in Toxicological Evaluation of Commonly Used Organophasphate Pesticides. Indian Journal of Experimental Biology, 44(7), 580-583.
27. Thapar, A., Sandhir, R., Kiran, R. 2002. Acephate Induced Oxidative Stress in Erythrocytes. Indian Journal of Experimental Biology, 40(8), 963-966.
28. Tomizawa, M., Casida, J.E. 2005. Neonicotinoid Insecticide Toxicology: Mechanisms of Selective Action. Annual Reviews in Pharmacology and Toxicology, 45, 247-268.
29. Yang, Z.P., Dettbarn, W.D. 1996. Diisopropyl Phosphofluoridate Induced Cholinergic Hyperactivity and Lipid Peroxidation. Toxicology and Applied Pharmacology, 138, 48-53.
30. Yu, M., Li, S.M., Li, X.Y., Zhang, B.J., Wang, J.J. 2008. Acute Effects of 1-Octyl-3-Methylimidazolium Bromide Ionic Liquid on the Antioxidant Enzyme System of Mouse Liver. Ecotoxicology and Environmental Safety, 71, 903-908.