Fabrication and Characterization of Polyvinyl Alcohol/Gelatin/ Silver Nanoparticle Nanocomposite Materials

Abstract

In this study, nanofibers containing silver nanoparticles were fabricated by electrospinnining method. The blends of polyvinyl alcohol as a synthetic polymer and gelatin as a biopolymer were used to fabricate wound dressing material having antibacterial activity. Scanning electron microscope (SEM) analysis indicated that porous and interconnected nanofibrous structures were obtained for all blend compositions. X-Ray diffraction (XRD) results showed the existence silver nanoparticles in the structure. The chemical changes related with crosslinking and the change of the structure in simulated body fluid were evaluated using Fourier transform infrared spectroscopy (FTIR). Antibacterial tests were performed to prove the antibacterial activity. The inductively coupled plasma optical emission spectrometry results showed that controlled silver release was achieved. It was concluded that obtained nanofiber mats could be suitable candidates for wound dressing applications

Keywords

• Silver Nanoparticles,Polyvinyl alcohol,Gelatin,Electrospinning

References

1. Abdelgawad AM, Hudson SM, and Rojas OJ 2014. Antimicrobial wound dressing nanofiber mats from multicomponent (chitosan/silver-NPs/polyvinyl alcohol) systems. Carbohydrate polymers, 100: 166-178.
2. Asran AS, Henning S, and Michler GH 2010. Polyvinyl alcohol–collagen–hydroxyapatite biocomposite nanofibrous scaffold: mimicking the key features of natural bone at the nanoscale level. Polymer, 51(4): 868-876.
3. Celebioglu A, Aytac Z, Umu OC, Dana A, Tekinay T, and Uyar T 2014. One-step synthesis of size-tunable Ag nanoparticles incorporated in electrospun PVA/cyclodextrin nanofibers. Carbohydrate polymers, 99: 808-816.Cheng Z, Zhang F, Liu W, Cui L, and Kang L 2015. A novel preparation for a PVA/l-histidine/AgNPs membrane and its antibacterial property. RSC Advances, 5(67): 54182-54187.
4. Chun JY, Kang HK, Jeong L, Kang YO, Oh J-E, Yeo I-S, Jung SY, Park WH, and Min B-M 2010. Epidermal cellular response to poly (vinyl alcohol) nanofibers containing silver nanoparticles. Colloids and Surfaces B: Biointerfaces, 78(2): 334-342.
5. Destaye AG, Lin CK, and Lee CK 2013. Glutaraldehyde vapor cross-linked nanofibrous PVA mat with in situ formed silver nanoparticles. ACS applied materials & interfaces, 5(11): 4745-4752.
6. Fathollahipour S, Abouei Mehrizi A, Ghaee A, and Koosha M 2015. Electrospinning of PVA/chitosan nanocomposite nanofibers containing gelatin nanoparticles as a dual drug delivery system. Journal of Biomedical Materials Research Part A, 103(12): 3852-3862.
7. Gao C, Gao Q, Li Y, Rahaman MN, Teramoto A, and Abe K 2013. In vitro evaluation of electrospun gelatin‐bioactive glass hybrid scaffolds for bone regeneration. Journal of Applied Polymer Science, 127(4): 2588-2599.
8. Ghasemzadeh H, and Ghanaat F 2014. Antimicrobial alginate/PVA silver nanocomposite hydrogel, synthesis and characterization. Journal of Polymer Research, 21(3): 355.

9. Gönen SÖ, Taygun ME, and Küçükbayrak S 2016. Fabrication of bioactive glass containing nanocomposite fiber mats for bone tissue engineering applications. Composite Structures, 13: 96-106.
10. Hong KH, Park JL, Sul IH, Youk JH, and Kang TJ 2006. Preparation of antimicrobial poly (vinyl alcohol) nanofibers containing silver nanoparticles. Journal of Polymer Science Part B: Polymer Physics, 44(17): 2468-2474.
11. Islam MS, and Yeum JH 2013. Electrospun pullulan/poly (vinyl alcohol)/silver hybrid nanofibers: preparation and property characterization for antibacterial activity. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 436: 279-286.
12. Kumar A, and Jaiswal M 2016. Design and in vitro investigation of nanocomposite hydrogel based in situ spray dressing for chronic wounds and synthesis of silver nanoparticles using green chemistry. Journal of Applied Polymer Science, 133(14):43260.
13. Li C, Fu R, Yu C, Li Z, Guan H, Hu D, Zhao D, and Lu L 2013. Silver nanoparticle/chitosan oligosaccharide/poly (vinyl alcohol) nanofibers as wound dressings: a preclinical study. International journal of nanomedicine, 8:4131.
14. Lin S, Wang RZ, Yi Y, Wang Z, Hao LM, Wu JH, Hu GH, and He H 2014. Facile and green fabrication of electrospun poly (vinyl alcohol) nanofibrous mats doped with narrowly dispersed silver nanoparticles. International journal of nanomedicine, 9:3937.Linh NTB, Min YK, Song HY, and Lee BT 2010. Fabrication of polyvinyl alcohol/gelatin nanofiber composites and evaluation of their material properties. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 95(1): 184-191.
15. Lu W, Ma M, Xu H, Zhang B, Cao X, and Guo Y 2015. Gelatin nanofibers prepared by spiral-electrospinning and cross-linked by vapor and liquid-phase glutaraldehyde. Materials Letters, 140(1-4.
16. Mahanta N, and Valiyaveettil S 2012. In situ preparation of silver nanoparticles on biocompatible methacrylated poly (vinyl alcohol) and cellulose based polymeric nanofibers. RSC Advances, 2(30): 11389-11396.
17. Martínez-Rodríguez M, Garza-Navarro M, Moreno-Cortez I, Lucio-Porto R, and González-González V 2016. Silver/polysaccharide-based nanofibrous materials synthesized from green chemistry approach. Carbohydrate polymers, 136: 46-53.
18. Merkle VM, Tran PL, Hutchinson M, Ammann KR, DeCook K, Wu X, and Slepian MJ 2015. Core–shell PVA/gelatin electrospun nanofibers promote human umbilical vein endothelial cell and smooth muscle cell proliferation and migration. Acta biomaterialia, 27: 77-87.
19. Nguyen TH, Lee KH, and Lee BT 2010. Fabrication of Ag nanoparticles dispersed in PVA nanowire mats by microwave irradiation and electro-spinning. Materials Science and Engineering: C, 30(7): 944-950.
20. Park JH, Kim IK, Choi JY, Karim MR, Cheong IW, Oh W, and Yeum JH 2011. Electrospinning fabrication of poly (vinyl alcohol)/waterborne polyurethane/silver composite nanofibre mats in aqueous solution for anti-bacterial exploits. Polymers & Polymer Composites, 19(9): 753.
21. Rujitanaroj P, Pimpha N, and Supaphol P 2008. Wound-dressing materials with antibacterial activity from electrospun gelatin fiber mats containing silver nanoparticles. Polymer, 49(21): 4723-4732.
22. Shalumon K, Anulekha K, Chennazhi KP, Tamura H, Nair S, and Jayakumar R 2011. Fabrication of chitosan/poly (caprolactone) nanofibrous scaffold for bone and skin tissue engineering. International journal of biological macromolecules, 48(4): 571-576.
23. Son WK, Youk JH, and Park WH 2006. Antimicrobial cellulose acetate nanofibers containing silver nanoparticles. Carbohydrate Polymers, 65(4): 430-434.
24. Sui C, Li C, Guo X, Cheng T, Gao Y, Zhou G, Gong J, and Du J 2012. Facile synthesis of silver nanoparticles-modified PVA/H4SiW12O40 nanofibers-based electrospinning to enhance photocatalytic activity. Applied Surface Science, 258(18): 7105-7111.
25. Şimşek M, Rzayev ZM, and Bunyatova U 2016. Multifunctional electroactive electrospun nanofiber structures from water solution blends of PVA/ODA–MMT and poly (maleic acid-alt-acrylic acid): Effects of Ag, organoclay, structural rearrangement and NaOH doping factors. Advances in Natural Sciences: Nanoscience and Nanotechnology, 7(2): 025009
26. Tsai RY, Hung SC, Lai JY, Wang DM, and Hsieh HJ 2014. Electrospun chitosan–gelatin–polyvinyl alcohol hybrid nanofibrous mats: Production and characterization. Journal of the Taiwan Institute of Chemical Engineers, 45(4): 1975-1981
27. Usman A, Hussain Z, Riaz A, and Khan AN 2016. Enhanced mechanical, thermal and antimicrobial properties of poly (vinyl alcohol)/graphene oxide/starch/silver nanocomposites films. Carbohydrate polymers, 153: 592-599.
28. Xiao S, Xu W, Ma H, and Fang X 2012. Size-tunable Ag nanoparticles immobilized in electrospun nanofibers: synthesis, characterization, and application for catalytic reduction of 4-nitrophenol. RSC Advances, 2(1): 319-327.
29. Yang C, Wu X, Zhao Y, Xu L, and Wei S 2011. Nanofibrous scaffold prepared by electrospinning of poly (vinyl alcohol)/gelatin aqueous solutions. Journal of Applied Polymer Science, 121(5): 3047-3055.
30. Yang D, Li Y, and Nie J 2007. Preparation of gelatin/PVA nanofibers and their potential application in controlled release of drugs. Carbohydrate Polymers, 69(3): 538-543.
31. Zhang Z, Wu Y, Wang Z, Zou X, Zhao Y, and Sun L 2016. Fabrication of silver nanoparticles embedded into polyvinyl alcohol (Ag/PVA) composite nanofibrous films through electrospinning for antibacterial and surface-enhanced Raman scattering (SERS) activities. Materials Science and Engineering: C, 69: 462-469.
32. Zhao Y, Zhou Y, Wu X, Wang L, Xu L, and Wei S 2012. A facile method for electrospinning of Ag nanoparticles/poly (vinyl alcohol)/carboxymethyl-chitosan nanofibers. Applied Surface Science, 258(22): 8867-8873.