TLC and GC-MS analysis of fermented wood "Nikhra" petroleum ether fraction of Combretaceae spp. Combretum hartmannianum and Terminalia laxiflora

Abstract

This study aims to analysis fractions (petroleum ether, chloroform, methanol  and aqueous) fermented wood Nikhra of Combretaceae spp (Combretum hartmannianum and Terminalia laxiflora)  analysis it by using chromatographic and spectroscopic analysis. Petroleum ether Nikhra fraction analysis with TLC and spray TLC with vanillin H₂SO₄ (pink) (B1, B2), Rf values (0.84, 0.81), were expected to be phenolic, with vanillin HCL (red) compounds spots (B2) with Rf values (0.81) was expected to be catechin and with vanillin H₃PO₄, blue-violet zones compounds spots (A1, A2, A3), (B1, B2, B3, B4), with Rf values (0.88, 0.78, 0.67), (0.84, 0.81, 0.67, 0.59), respectively were expected to be lignans. Petroleum ether fermented wood"Nikhra"  fraction was divided into two types of compounds classes aromatic and non aromatic by  and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS analysis gave a spectrum of fragrance aromatic compounds (phenolics) in the petroleum ether Nikhrafractions of T. laxiflora, was Lup-20(29)-en-3-ol, acetate, (3β) and Tetracosamethyl-cyclododecasiloxane, main terponoids compounds was eicosamethylcyclodecasiloxane. Main fragrance aromatics compounds in the petroleum ether Nikhra fraction of C. hartmannianum was 2-tert-Butyl-5-(hydroxtmethyl)-4-formylfuran, and main terponoids compounds was Tetracosamethylcyclododecasiloxane.

Keywords

• Combretaceae,Combretum hartmannianum,Terminalia laxiflora,Gas Chromatography

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