TLC and GC-MS analysis of fermented wood "Nikhra" petroleum ether fraction of Combretaceae spp. Combretum hartmannianum and Terminalia laxiflora

Yıl 2018, , 1 - 7, 26.10.2018

Noha Fadle Abdalbasit Mariod Hiba Abdel Rahman Ali Alfatih Ahmed Hasan

https://doi.org/10.31195/ejejfs.417112

Öz

This study aims to analysis
fractions (petroleum ether, chloroform,
methanol  and
aqueous) fermented
wood Nikhra of Combretaceae spp (Combretum hartmannianum and Terminalia laxiflora)  analysis it by using
chromatographic and spectroscopic analysis. Petroleum ether Nikhra fraction analysis with TLC and spray TLC with
vanillin H₂SO₄
(pink) (B1, B2), Rf values (0.84, 0.81), were expected to be phenolic, with vanillin HCL (red)
compounds spots (B2) with Rf values (0.81) was expected to be catechin and with vanillin H₃PO₄,
blue-violet zones compounds spots
(A1, A2, A3), (B1, B2, B3, B4), with Rf values (0.88, 0.78, 0.67), (0.84, 0.81, 0.67, 0.59), respectively
were expected to be lignans. Petroleum ether
fermented
wood"Nikhra"  fraction was divided into two types of compounds
classes aromatic and non aromatic by  and hence
compounds were classified to phenolics and terpenoids
compounds by
GC/MS.
Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS
analysis gave a spectrum of fragrance aromatic compounds (phenolics) in the
petroleum ether Nikhrafractions of T. laxiflora, was
Lup-20(29)-en-3-ol, acetate, (3β) and Tetracosamethyl-cyclododecasiloxane, main
terponoids compounds was eicosamethylcyclodecasiloxane. Main fragrance aromatics compounds in the petroleum ether Nikhra fraction of C. hartmannianum was 2-tert-Butyl-5-(hydroxtmethyl)-4-formylfuran,
and main terponoids compounds was Tetracosamethylcyclododecasiloxane.

Anahtar Kelimeler

Combretaceae, Combretum hartmannianum, Terminalia laxiflora, Gas Chromatography

Kaynakça

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