TLC and GC-MS analysis of fermented wood "Nikhra" petroleum ether fraction of Combretaceae spp. Combretum hartmannianum and Terminalia laxiflora

Öz

This study aims to analysis fractions (petroleum ether, chloroform, methanol  and aqueous) fermented wood Nikhra of Combretaceae spp (Combretum hartmannianum and Terminalia laxiflora)  analysis it by using chromatographic and spectroscopic analysis. Petroleum ether Nikhra fraction analysis with TLC and spray TLC with vanillin H₂SO₄ (pink) (B1, B2), Rf values (0.84, 0.81), were expected to be phenolic, with vanillin HCL (red) compounds spots (B2) with Rf values (0.81) was expected to be catechin and with vanillin H₃PO₄, blue-violet zones compounds spots (A1, A2, A3), (B1, B2, B3, B4), with Rf values (0.88, 0.78, 0.67), (0.84, 0.81, 0.67, 0.59), respectively were expected to be lignans. Petroleum ether fermented wood"Nikhra"  fraction was divided into two types of compounds classes aromatic and non aromatic by  and hence compounds were classified to phenolics and terpenoids compounds by GC/MS. Fragrant aromatics or terpenoids were targeted in this part of study. GC-MS analysis gave a spectrum of fragrance aromatic compounds (phenolics) in the petroleum ether Nikhrafractions of T. laxiflora, was Lup-20(29)-en-3-ol, acetate, (3β) and Tetracosamethyl-cyclododecasiloxane, main terponoids compounds was eicosamethylcyclodecasiloxane. Main fragrance aromatics compounds in the petroleum ether Nikhra fraction of C. hartmannianum was 2-tert-Butyl-5-(hydroxtmethyl)-4-formylfuran, and main terponoids compounds was Tetracosamethylcyclododecasiloxane.

Anahtar Kelimeler

• Combretaceae,Combretum hartmannianum,Terminalia laxiflora,Gas Chromatography

Kaynakça

1. Abbiw, D. K. (1990). "Useful plants of Ghana: West African uses of wild and cultivated plants," Intermediate Technology Publications and The Royal Botanic Gardens. Journal of Tropical Ecology 7 286-287
2. Ali, H., König, G., Khalid, S., Wright, A., and Kaminsky, R. (2002). Evaluation of selected Sudanese medicinal plants for their in vitro activity against hemoflagellates, selected bacteria, HIV-1-RT and tyrosine kinase inhibitory, and for cytotoxicity. Journal of ethnopharmacology 83, 219-228.
3. Batawila, K., Kokou, K., Koumaglo, K., Gbeassor, M., De Foucault, B., Bouchet, P., and Akpagana, K. (2005). Antifungal activities of five Combretaceae used in Togolese traditional medicine. Fitoterapia 76, 264-268.
4. Daniel, K. A. (1990). Useful plants of Ghana: West African uses of wild and cultivated plants. Intermediate Tech. Pub. Ltd and Royal Botanic Gardens, Kew, 337.
5. Doka, I., and Yagi, S. (2009). Ethnobotanical survey of medicinal plants in west Kordofan (Western Sudan). Ethnobotanical Leaflets 2009, 8.
6. Fyhrquist, P., Mwasumbi, L., Hæggström, C.-A., Vuorela, H., Hiltunen, R., and Vuorela, P. (2002). Ethnobotanical and antimicrobial investigation on some species of Terminalia and Combretum (Combretaceae) growing in Tanzania. Journal of Ethnopharmacology 79, 169-177.
7. Mariod, A. A., Mohammed, N. M. F., Nabag, F. O., and Hassan, A. A. (2014). Ethnobotanical study of three trees: indigenous knowledge on trees used as cosmetic in Khartoum State, Sudan. Eur. J. Mol. Biol Biochem. 1 (2): 77-80
8. Wagner, H., and Bladt, S. (1996). Plant drug analysis: a thin layer chromatography atlas, Springer Science & Business Media.