Antinociceptive Activity of Ballota glandulosissima Hub. -Mor & Patzak

Abstract

Abstract. We aimed to investigate antinociceptive activity and median lethal dose (LD₅₀) of water extract of Ballota glandulosissima Hub.-Mor&Patzak in mice. In this study, water extract of Ballota glandulosissima was investigated for antinociceptive activity using acetic acid-induced “writhing” and “tail-flick” tests. Acetyl salicylic acid and morphine were used as the reference drug. Mice were injected Ballota glandulosissima extract intraperitoneally in doses of 100 mg/kg and 200 mg/kg, respectively. The extract of Ballota glandulosissima caused dose related inhibition in the acetic acid-induced ab dominal stretching response in mice. The extract of Ballota glandulosissima also showed significant changes in the nociceptive threshold of the tail-flick test. The motor coordination of mice treated with the water extract was evaluated by using the “rotarod” test and was found to be not impaired in comparison with that of control mice.The results obtained in the present study indicate that the water extract of Ballota glandulosissima has promising antinociceptive activity. The LD₅₀ of Ballota glandulosissima was determined as 8.885 g/kg.

Keywords: Ballota glandulosissima, lamiaceae, antinociception, writhing test, tail-flick test, rotarod test.

Keywords

• Ballota glandulosissima, lamiaceae, antinociception, writhing test, tail-flick test, rotarod test

References

1. Davis PH: Flora of Turkey and the East Aegean Islands. Vol 7, Edinburgh, Edinburgh University Press, 1982, pp. 156-160.
2. Baytop T: Türkiye’de Bitkilerle Tedavi (Therapy with Me-dicinal Plants in Turkey), İstanbul, Nobel Tıp Kitapevi, 1999, p. 371.
3. Çitoğlu G, Tanker M, Sever B, Englert J, Anton R, Altanlar N: Antibacterial activities of diterpenoids isolated from B.saxatilis subsp. saxatilis. Planta Med 1998;64: 484.
4. Vural K, Ezer N, Erol K, Şahin FP: Anxiolytic and antide-pressant activities of some Ballota species. J Fac Pharm Gazi 1996;13: 29-32.
5. Meriçli AH, Meriçli F, Tuzlacı E: Flavonoids of Ballota acetabulosa. Acta Pharm Turcica 1988;30:
6. Yeşilada E, Honda G, Sezik E, Tabata M, Goto K, Ikeshiro Y: Traditional medicine in the Mediterranean subdivision. J Ethnopharmacol 1993;39: 31-38.
7. Yeşilada E, Honda G, Sezik E et al: Traditional medicine in Turkey V. Folk medicine in the inner Taurus Mountains. J Ethnopharmacol 1995;46: 133-152.
8. Tuzlacı E, Tolon E: Turkish folk Medicinal plants, part III: Şile (İstanbul). Fitoterapia 2000;71: 673- 685.

9. Çitoğlu G, Yılmaz BS, Altanlar N: Antimicrobial activity of Ballota species growing in Turkey, AÜ Ecz Fak Der 32(2): 2003 (in press).
10. Çitoğlu GS, Sever B, Antus S, Baitz-Gacs E, Altanlar N: Antifungal flavonoids from Ballota glandulosissima. Pharm Biol 41: 2003 (in Press)
11. Sever B: The investigation of diterpenoid and flavonoid contents of Ballota species growing in Turkey, PhD Thesis An-kara, 2000.
12. Çitoğlu G, Tanker M, Sever B: Flavonoid aglycones from B.saxatilis subsp. saxatilis. Pharm Biol 1999;37: 158.
13. Kouadio F, Kanko C, Juge M et al: Analgesic and antiinflammatory activities of an extract from Parkia biglobosa used in traditional medicine in the Ivory Coast. Phytother Res 2000;14:635.
14. Litchfield JT, Wilcoxon FW: A simplified method of evaluating dose-effect experiments, J Pharmacol Exp Ther 1949;96: 99-113.
15. Abdel-Barry JA, Abdel-Hassan IA, Al-Hakiem MHH: Hypoglycaemic and antihyperglycaemic effects of Trigonella foenumgraecum leaf in normal and alloxan induced diabetic rats. J Ethnopharm 1997;58: 149-155.
16. Koster R, Anderson M, De Beer EJ: Acetic acid for analgesic screening. Fed Proc 1959;18: 412.
17. Hunskaar S, Fasmer OB, Hole K: Formalin test in mice, a useful technique for evaluating mild analgesics. J Neurosci Methods 1985;14(1): 69-76.
18. Tanker M, Çitoğlu G, Gümüşel B, Şener B: Alkaloids of Sternbergia clusiana and their analgesic effects. Int J of Pharmacognosy 1996;34: 194-198.
19. D’Amour FE, Smith DL: A method for determining loss of pain sensation. Pharmacol J Exp Ther 1941;72: 74-79.
20. Matsumoto K, Horie S, Ishikawa H, Takayama H, Aimi N, Ponglux D, Watanabe K: Antinociceptive effect of 7-hydroxymitragynine in mice: Discovery of an orally active opioid analgesic from the Thai medicinal herb Mitragyna speciosa. Life Sciences 2004;74: 2143-2155.
21. Souza F, Fighera MR, Lima TTF et al: 3-Methyl-5- hydroxymethyl-5-trichloromethyl-1H-1- pyrazolcarboxamide induces antinociception. Pharmacol Biochem Behav 2001;68: 525-530.
22. Sümbüloğlu K, Sümbüloğlu V: Biostatistics. 8thedition, Ankara, Hatiboğlu Yayınevi, 1998, pp. 76-86.
23. Ferreres F, Tomas-Barberan FA, Tomas-Lorente F: Flavonoidcompounds from Ballota hirsuta. J Nat Prod 1986;49:554-555.
24. Seidel V, Bailleul F, Tillequin F: Phenylpropanoid glycosidesfrom Ballota nigra. Planta Med 1995;62: 186-187.
25. Bertrand MC, Tillequin F, Bailleul F: Two major flavonoids from Ballota nigra. Biochem Syst Ecol 2000; 28: 1031-1033.
26. Sahpaz S, Skaltsounis AL, Bailleul F: Polyphenols from Ballota acetabulosa. Biochem Syst Ecol 2002;30: 601-604.
27. Di Carlo G, Mascolo N, Izzo AA, Carpasso F: Flavonoids:Old and new aspects of a class of natural therapeutic drugs.Life Sciences 1999;65: 337-353.
28. Harborne JB, Williams CA: Advances in flavonoid research since 2000;1992. Phytochemistry 55: 481