 The effect of indole-3-acetic acid on some metabolic enzymes in kidney of the second cross maternal mice and their offsprings

Year 2004, Volume: 9 Issue: 1, 1 - 3, 27.02.2013

Yılmaz H. Ramazan Yüksel Eşref

Abstract

Objective: Indole-3-acetic acid (IAA) generates reactive
oxygen species (ROS) and excited oxygen
intermediates. IAA causes renal dysfunction,
hypoglycemia, and myotonia. The first aim of the study
is to investigate the possible indirect effect of the plant
growth hormone IAA on some renal enzyme activities
such as hexokinase (HK), glucose-6-phosphate
dehydrogenase (G6PD), 6-phosphogluconate
dehydrogenase (6PGD), lactate dehydrogenase (LDH),
and malate dehydrogenase (MDH) of second cross
offsprings of the second cross maternal mice (Mus
musculus) that are given IAA. The second aim was to
investigate the possible direct effect of IAA on the same
renal enzymes in second cross maternal mice.
Method: Female mice was divided into two groups: IAA
administered group and ethanol control group. Two
chemicals used were applied intraperitoneally. IAA was
administrated to maternal mice as a 1/40 dilution of
300 mg/kg body weights in 3-day intervals. Ethanol was
administrated in the controls. Spectrophotometric
methods were used to determine the activities of
enzymes in the kidney of the second cross-maternal
mice and their offsprings.
Results: In the second cross-maternal mice, the
activities of LDH, MDH, and G6PD were higher in IAA
administrated animals than in controls. 6PGD and HK
showed  decreases in IAA study group compared to
control group. In the offsprings of the second cross
maternal mice, the activities of G6PD, HK, and MDH
enzymes were decreased, and 6PGD and LDH enzymes
were increased in IAA group compared to control
group. Even so differences were not statistically
significant.
Conclusion:  The results obtained suggested that
metabolic enzymes studied were not affected from IAA
toxicity. This may be due to low dose of IAA.
Key words: IAA, metabolic enzymes,  mus musculus,
kidney.

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