Piroksikamın pKa Değerinin Yüksek Performanslı Sıvı Kromatografisi ile Belirlenmesi

Year 2021, , 711 - 715, 31.12.2021

Murat Soyseven , Burcu Sezgin , Göksel Arli

https://doi.org/10.31590/ejosat.1039852

Abstract

Bu çalışmada bir nonsteroid anti-inflamatuvar ilaç olan piroksikamın ters faz sıvı kromatografi yöntemiyle farklı asetonitril-su ikili karışımlarındaki ve sudaki asidik iyonlaşma sabitleri (pKa) belirlenmiştir. Piroksikamın kromatografik davranışına hareketli fazın pH’ı ve çözücü yüzdesinin birleştirilmiş etkisini incelemek amacıyla SVEA C18 (5 μm, 150 mm x 4.6 mm) YPSK analiz kolonu ve farklı oranda asetonitril-su (%30, %35 ve %40 (h/h)) ikili karışımlarından oluşan, farklı pH değerlerine (3,13-6,18) sahip hareketli fazlar kullanılmıştır. Asidik iyonlaşma sabitleri doğrusal olmayan regresyon modeli kullanılarak tahmin edilmiştir. Piroksikamın sudaki pKa değeri mol kesri-pKa yöntemiyle hesaplanmıştır. Veriler literatürde farklı tekniklerle elde edilmiş olanlarla kıyaslanmış ve uyumlu olduğu görülmüştür.

Keywords

pka, Piroksikam, Yüksek Performanslı Sıvı Kromatografisi

Determination of pKa Value of Piroxicam by High Performance Liquid Chromatography

Abstract

In this study, acid ionization constants (pKa) of piroxicam, a nonsteroidal anti-inflammatory drug, in different acetonitrile-water binary mixtures and water were determined by reverse phase liquid chromatography method. SVEA C18 (5 μm, 150 mm x 4.6 mm) HPLC analysis column and different ratios of acetonitrile-water (30%, 35%, and 40% (v/v)) binary mixtures with different pH values (3.13-6.18) were used to investigate the combined effect of pH of the mobile phase and percentage of solvent on the chromatographic behavior of piroxicam. Acidic ionization constants were estimated using a nonlinear regression model. The pKa value of piroxicam in water was calculated by the mole fraction- pKa method. The data were compared with values obtained by different techniques in the literature and were found to be compatible.

Keywords

pka, Piroxicam, High Performance Liquid Chromatography

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