Mikrobiyal olarak indüklenen kalsiyum karbonat çökeltisinin potansiyel kullanımı için Sporosarcina pasteurii' nin üreaz aktivitesinin araştırılması

Year 2022, , 1 - 4, 31.03.2022

Kağan Eryürük

https://doi.org/10.31590/ejosat.1061497

Abstract

Sporosarcina pasteurii'nin üreaz aktivitesi, farklı sayıda bakteri hücresi kullanılarak araştırıldı. Çözeltinin elektriksel iletkenliğindeki değişimi ölçmek için dinlenme halindeki Sporosarcina pasteurii hücreleri ve 500 mM üre çözeltisi beherlere eklendi. Elektriksel iletkenlik değerleri 10 dakika süreyle 0.382 mS/m ile 6.224 mS/m arasında değişiklik göstermiştir. En yüksek elektriksel iletkenlik, çözeltiye en fazla sayıda hücrenin (10*109) eklenmesi için ölçülmüştür. Dinlenme halindeki Sporosarcina pasteurii hücreleri ve 500 mM üre ve 500 mM CaCl2 çözeltisi de mikrobiyal olarak indüklenen CaCO3 çökeltisini gözlemlemek ve değerlendirmek için beherlere yerleştirildi. On dakikalık deneylerde mikrobiyal olarak indüklenen CaCO3 çökeltisi miktarı 4 mg ile 28 mg arasında ölçülmüştür. En yüksek CaCO3 çökeltisi miktarı, en az hücre sayısına (4 mg) kıyasla en fazla hücre sayısına sahip beherde (28 mg) gözlendi. Bu nedenle, mikrobiyal olarak indüklenen CaCO3 çökeltisi miktarının, beherlerdeki hücre sayısıyla doğru orantılı olarak arttığı söylenebilir. Deneyler, S. pasteurii'nin üreaz aktivitesinin hücre sayısına bağlı olduğunu ve daha fazla S. pasturii hücresinin kısa bir süre içinde daha fazla mikrobiyal olarak indüklenen CaCO3 çökeltisine yol açabileceğini göstermektedir.

Keywords

Elektriksel iletkenlik, Hücre sayısı, Mikrobiyal olarak indüklenen CaCO3 çökeltisi, Sporosarcina pasteurii, Sporosarcina pasteurii, Üreaz aktivitesi

Investigating the urease activity of Sporosarcina pasteurii for potential usage of microbially induced calcium carbonate precipitation

Abstract

The urease activity of Sporosarcina pasteurii was investigated using different number of bacterial cells. Resting Sporosarcina pasteurii cells and 500 mM urea solution were placed into the beakers to measure the change in electrical conductivity of the solution. The electrical conductivity values were varied between 0.382 mS/m and 6.224 mS/m for 10 min. The highest electrical conductivity was measured for addition of the largest number of cells (10*109) to the solution. Resting Sporosarcina pasteurii cells and 500 mM urea and 500 mM CaCl2 solution were also placed into the beakers to observe and evaluate microbially induced CaCO3 precipitation. The amount of microbially induced CaCO3 precipitation was changed between 4 mg and 28 mg in the experiments for ten minutes. The highest amount of CaCO3 precipitation was observed in beaker with the highest number of cells (28 mg) than in those with the smallest number of cells (4 mg). Hence, the amount of microbially induced CaCO3 precipitation was increased in direct proportion to the cell number in the beakers. The experiments suggest that the urease activity S. pasteurii is depended on the number of cells and more cells of S. pasteurii leads more microbially induced CaCO3 precipitation in a short time period.

Keywords

Electrical conductivity, Microbially induced calcium carbonate precipitation, Number of cells, Sporosarcina pasteurii, Urease activity

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