Sağlıklı ve preeklamptik gebelerde oksidatif stres parametrelerinin ve nitrik oksitin değerlendirilmesi

Öz

Bu çalışmada preeklamptik gebelerde oksidatif hasar parametreleri araştırıldı ve sağlıklı dokularla karşılaştırıldı. 53 preeklamptik ve 53 sağlıklı gebe plasentada antioksidan enzimler belirlendi. Plasenta dokusunda serbest oksijen radikallerinin hasarı, antioksidan enzim sistemleri (lipid peroksidasyon (LPO), glutatyon (GSH) ve katalaz (CAT) miktarları, miyeloperoksidaz (MPx) enzim aktiviteleri ve nitrik oksit düzeyleri ölçülerek belirlendi. Preeklampsi geçirmiş gebede LPO düzeyleri sağlıklı gebelere göre yükselmiştir. Sağlıklı plasenta dokularında GSH miktarı yüksek iken, preeklampsi dokularında oldukça düşük bulundu. Preeklampsili kadınların plasental dokularında CAT, MPx enzim aktiviteleri ve NO düzeyleri oldukça yüksek iken, sağlıklı dokularda düşük bulunmuştur. Plasental dokularda antioksidan enzim aktiviteleri ve miktarları gruplar arasında önemli farklılıklar göstermektedir. Bu veriler doğrultusunda oksidatif stres parametrelerinin doku düzeyindeki hasarı oldukça iyi gösterdiğini söyleyebiliriz. Sonuç olarak elde edilen veriler plasenta dokusunda oksidatif hasarı göstermekte ve antioksidan mekanizmanın çalıştığını göstermektedir.

Anahtar Kelimeler

• Preeklampsi
• Oksidatif hasar
• Reaktif oksijen türleri
• antioksidan enzimler

The evaluation of oxidative stress parameters and nitric oxide in healthy and preeclamptic pregnant women

Abstract

In the present study, oxidative damage parameters in preeclamptic pregnant women were investigated and compared with healthy tissues. Antioxidant enzymes were determined in 53 preeclamptic and 53 healthy pregnant placentas. The damage of free oxygen radicals in the placental tissue was determined by measuring by antioxidant enzyme systems (The amounts of lipid peroxidation (LPO), glutathione (GSH) and catalase (CAT), myeloperoxidase (MPx) enzyme activities and nitric oxide levels (NO)). The LPO levels increased in the pregnant woman who has had preeclampsia compared with healthy pregnant. While the amount of GSH was high in healthy placental tissues, it was found to be quite low preeclampsia tissues. While CAT, MPx enzyme activities and levels of NO were quite high in the placental tissues of women with preeclampsia, they were found to be low in healthy tissues. The antioxidant enzyme activities and amounts show significant differences between groups in placental tissues. In line with these data, we can say that oxidative stress parameters show the damage at the tissue level quite well. As a result, the data obtained show oxidative damage in the placental tissue and indicate that the antioxidant mechanism works.

Keywords

• Preeclampsia
• Oxidative damage
• Reactive oxygen species
• Antioxidant enzymes

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