The Effects of Acetretin-Methotrexate Combination and Α-Lipolic Acid on Polyphenol Oxidase Activity in Rat Brain Tissue

Abstract

In this study, the effects of acitretin (ACT) and methotrexate (MTX) active ingredients and alpha lipoic acid (ALA) on Polyphenol Oxidase (PO) activity in rat brain tissue were investigated. A total of 50 Wistar albino male rats were used in the study. Study groups were formed as Control group (K), ALA group, ACT+MTX group and ACT+MTX+ALA group. Injections made to rats that were fasted for 24 hours were performed at the same time each morning. ACT, MTX and ALA were dissolved in 0.9% NaCl. ACT (20mg/kg/day), MTX (20mg/kg/week), ALA (50mg/kg/day) and their combinations were given to the rats by intraperitoneal injection at body weight level. Rats were sacrificed by cervical dislocation and their brains were removed after cardiac perfusion. PO enzyme activity was measured in brain tissue samples taken from rats. While In the group given ACT+MTX, approximately 5% inhibition was observed on the 3rd day compared to K, this inhibition was replaced by activation with the effect of ALA. While the use of ACT + MTX caused inhibition in brain PO activity on the 3rd day, this inhibition turned into activation in the following days. When the group given ALA alone was compared with the K group; PO activity showed 7% inhibition on 3rd day. It was observed that this inhibition level decreased by half on the 5th and 7th days. Thus, as a result of our study, it can be said that ALA has an antioxidant effect in the brain tissue.

Keywords

• Brain,
• Acitretin,
• Methotrexate
• Polyphenol oxidase

Rat Beyin Dokusunda Asitretin - Metotreksat Kombinasyonunun ve Α-Lipolik Asit’in Polifenol Oksidaz Aktivitesi Üzerine Etkileri

Abstract

Bu çalışmada asitretin (ACT) ve metotreksat (MTX) etken maddelerinin ve alfa lipoik asitin (ALA) rat beyin dokusunda Polifenol Oksidaz (PO) aktivitesine etkileri araştırılmıştır. Çalışmada, toplam 50 tane Wistar albino erkek rat kullanılmıştır. Çalışma grupları, Kontrol grubu (K), ALA grubu, ACT+MTX grubu ve ACT+MTX+ALA grubu olarak oluşturulmuştur. 24 saat aç bırakılmış olan ratlara yapılan enjeksiyon işlemleri her sabah aynı saatte gerçekleştirilmiştir. ACT, MTX ve ALA % 0.9’luk NaCl’de çözülmüştür. ACT (20mg/kg/gün), MTX (20mg/kg/hafta), ALA (50mg/kg/gün) ve bunların kombinasyonları da vücut ağırlığı düzeyinde intraperitonal enjeksiyon ile ratlara verilmiştir. Ratlar servikal dislokasyon ile sakrifiye edilmiş ve kalp perfüzyonundan sonra beyinleri çıkarılmıştır. Ratlardan alınan beyin doku örneklerinde, PO enzim aktivitesi ölçülmüştür. ACT+MTX verilen grupta 3. günde K’ya göre yaklaşık % 5 inhibisyon gözlenirken ALA’nın etkisi ile bu inhibisyon yerini aktivasyona bırakmıştır. ACT + MTX kullanımı beyin PO aktivitesinde 3. günde inhibisyona neden olurken bu inhibisyon ilerleyen günlerde aktivasyona dönüşmüştür. Tek başına ALA verilen grup K grubu ile karşılaştırıldığında; PO aktivitesi 3. günde %7 inhibisyon göstermiştir. 5. ve 7.günlerde inhibisyon seviyesinin yarı yarıya azaldığı gözlenmiştir. Böylece çalışmamızın sonucu olarak ALA’nın beyin dokusunda antioksidan etki gösterdiği söylenebilir.

Keywords

• Beyin,
• Asitretin,
• Metotreksat,
• Polifenol oksidaz

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