Genotoxic and Antigenotoxic Effects of Some Plant Species of Lamiaceae Family

Abstract

Lamiaceae family is one of the members of important plants, using as a agents for alternative medicine treatments. Our aim was to investigate both antigenotoxic and genotoxic effects three different plants species belonging to Lamiaceae family: Stachys annua, Scutellaria salviifolia, Nepeta nuda in Amasya, using SMART (Drosophila melanogaster somatic mutation and recombination test) and also plasmid DNA interaction method, respectively. SMART analyses revealed that genotoxic effect of ethyl methanesulfonate (EMS) was eliminated by plant extract of S. salviifolia, indicating antigenotoxic effects.  Different extract concentrations [(0.1, 0.25, 0.50 and 1 % (v/v)] of other plants species decrased the flecks number on D. melanogaster but the results did not show any statistical significance (p>0.05). In addition to SMART, interaction with pUC18 plasmid DNA was also analysed by using all concentrations (50 µg/mL-3.125 µg/mL) of plant extracts N. nuda and S. annua. The extracts did not cause changed in Form I DNA (supercoil) but trasferred from Form II DNA (open circular) to Form III DNA. Moreover, there was no effect of S. salviifolia plant extracts on DNA and band density. SMART results were supported by DNA interaction analyses, cocncluding that S. salviifolia plants indicated the high-impact antigenotoxic effect, but not genotoxic effects.

Keywords

• Lamiaceae,Drosophila melanogaster,plasmid DNA

Lamiaceae Ailesine ait Bazı Bitki Türlerinin Genotoksik ve Antigenotoksik Etkileri

Öz

Lamiaceae ailesi, alternatif tıp tedavileri için ajan olarak kullanılan önemli bitki üyelerinden biridir. Çalışmada; Amasya’da bulunan Laminaceae familyasına ait üç farklı bitki türünün (Stachys annua, Scutellaria salviifolia, Nepeta nuda) antigenotoksik (SMART, Drosophila melanogaster somatik mutasyon ve rekombinasyon testi)  ve genotoksik (plazmid DNA etkileşim metodu) etkilerinin belirlenmesi amaçlanmıştır.

SMART analizleri, Etil metansülfonatın (EMS) genotoksik etkisinin, Scutellaria salviifolia bitki özütü ile ortadan kaldırıldığını ve antigenotoksik etkilerin bulunduğunu göstermiştir. Diğer bitki türlerinin farklı ekstrakt konsantrasyonları [(0.1, 0.25, 0.50 ve % 1 (v/v)], D. melanogaster üzerindeki lekelerin sayısını azaltmış ancak sonuçlar istatistiksel olarak anlamlı bulunmamıştır (p>0.05). SMART'a ek olarak, pUC18 plazmid DNA ile etkileşim de, N. nuda ve S. annua bitki özütlerinin (50 µg/mL-3.125 µg/mL) tüm konsantrasyonları kullanılarak analiz edilmiştir. Ekstraktlar Form I DNA'sında (supercoil) değişime neden olmamış, ancak Form II DNA'dan (açık dairesel) Form III DNA'ya aktarılmıştır. Ayrıca, S. salviifolia bitki ekstreleri DNA ve bant yoğunluğunu etkilememiştir. SMART sonuçları, S. salviifolia bitkilerinin yüksek etkili antijenotoksik etki gösterdiğini ancak genotoksik etkilerin olmadığını gösteren DNA etkileşimi analizleri ile desteklenmiştir.

Anahtar Kelimeler

• Lamiaceae,Drosophila melanogaster,plazmit DNA

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