Chromatographic Applications in Medicine

Abstract

Chromatography has been preferred in the medical field as one of the most important analytical methods used for the identification and quantification of a drug and its metabolites in recent years. Various chromatographic techniques have been developed to differentiate drugs according to their characteristics and interaction types. Among these techniques, particularly High Performance Affinity Chromatography (HPAC), Cell Membrane Chromatography (CMC), Mixed Mode Chromatography (MMC), High Performance Liquid Chromatography (HPLC) are used for pharmaceutical purposes for bioanalysis of drugs in preclinical and clinical studies. The success of Chromatography in the development of rapid and precise analytical methods provides superior specificity and sensitivity in the research of pharmacokinetic properties of drugs in R&D. In personalized medicine, it is important how the dosage and effect of drugs varies from person to person. In recent years, chromotography has been preferred as a promising technique in the analysis of drug-protein binding and in the examination of clinical or pharmaceutical samples.

Keywords

• Chromatography
• biopharmaceutics
• drug interaction

References

1. Denoroy, L., Zimmer, L., Renaud, B., & Parrot, S. (2013). Ultra high performance liquid chromatography as a tool for the discovery and the analysis of biomarkers of diseases: a review. Journal of Chromatography B, 927, 37-53.
2. Desfontaine, V., Guillarme, D., Francotte, E., & Nováková, L. (2015). Supercritical fluid chromatography in pharmaceutical analysis. Journal of pharmaceutical and biomedical analysis, 113, 56-71.
3. Ding, X., Chen, X., Cao, Y., Jia, D., Wang, D., Zhu, Z.,& Chai, Y. (2014). Quality improvements of cell membrane chromatographic column. Journal of Chromatography A, 1359, 330-335.
4. Du Toit, T., Bloem, L. M., Quanson, J. L., Ehlers, R., Serafin, A. M., & Swart, A. C. (2017). Profiling adrenal 11β-hydroxyandrostenedione metabolites in prostate cancer cells, tissue and plasma: UPC2-MS/MS quantification of 11β-hydroxytestosterone, 11 keto-testosterone and 11 keto-dihydrotestosterone. The Journal of steroid biochemistry and molecular biology, 166, 54-67.
5. Fan, J., Luo, J., & Wan, Y. (2017). Aquatic micro-pollutants removal with a biocatalytic membrane prepared by metal chelating affinity membrane chromatography. Chemical Engineering Journal, 327, 1011-1020.
6. Fassauer, G. M., Hofstetter, R., Hasan, M., Oswald, S., Modeß, C., Siegmund, W., & Link, A. (2017). Ketamine metabolites with antidepressant effects: fast, economical, and eco-friendly enantioselective separation based on supercritical-fluid chromatography (SFC) and single quadrupole MS detection. Journal of pharmaceutical and biomedical analysis, 146, 410-419.
7. Felletti, S., Ismail, O. H., De Luca, C., Costa, V., Gasparrini, F., Pasti, L.,& Catani, M. (2019). Recent achievements and future challenges in supercritical fluid chromatography for the enantioselective separation of chiral pharmaceuticals. Chromatographia, 82(1), 65-75.
8. Ghose, S., & Kumpalume, P. (2003). Chromatography: the high-resolution technique for protein separation. In Isolation and Purification of Proteins (pp. 44-69). CRC Press.

9. Hage, D. S.(2006). Handbook of affinity chromatography. 2nd edition. Volume: 92.
10. Hage, D. S., Anguizola, J. A., Jackson, A. J., Matsuda, R., Papastavros, E., Pfaunmiller, E.,& Zheng, X. (2011). Chromatographic analysis of drug interactions in the serum proteome. Analytical Methods, 3(7), 1449-1460.
11. Hage, D. S., Jackson, A., Sobansky, M. R., Schiel, J. E., Yoo, M. J., & Joseph, K. S. (2009). Characterization of drug–protein interactions in blood using high‐performance affinity chromatography. Journal of separation science, 32(5‐6), 835-853.
12. He, L. C., Wang, S. C., Yang, G. D., Zhang, Y. M., Wang, C. H., Yuan, B. X., & Hou, X. F. (2007). Progress in cell membrane chromatography. Drug Discov Ther, 1(2), 104-107.
13. Higgins V, Nieuwesteeg M, Adeli K. (2016). Reference Intervals: Theory and Practice. Clarke W, editor. Contemporary Practice in Clinical Chemistry, 3rd ed: AACC Press, 21-36.
14. Hochepied, T., Wullaert, A., Berger, F. G., Baumann, H., Brouckaert, P., Steidler, L., & Libert, C. (2002). Overexpression of α1-acid glycoprotein in transgenic mice leads to sensitisation to acute colitis. Gut, 51(3), 398-404.
15. Hou, X., Zhou, M., Jiang, Q., Wang, S., & He, L. (2009). A vascular smooth muscle/cell membrane chromatography–offline-gas chromatography/mass spectrometry method for recognition, separation and identification of active components from traditional Chinese medicines. Journal of Chromatography A, 1216(42), 7081-7087.
16. Hou, X., Wang, S., Zhang, T., Ma, J., Zhang, J., Zhang, Y., ... & He, L. (2014). Recent advances in cell membrane chromatography for traditional Chinese medicines analysis. Journal of pharmaceutical and biomedical analysis, 101, 141-150.
17. Kalogria, E., Pistos, C., & Panderi, I. (2013). Hydrophilic interaction liquid chromatography/positive ion electrospray ionization mass spectrometry method for the quantification of alprazolam and α-hydroxy-alprazolam in human plasma. Journal of Chromatography B, 942, 158-164.
18. Kortz, L., Helmschrodt, C., & Ceglarek, U. (2011). Fast liquid chromatography combined with mass spectrometry for the analysis of metabolites and proteins in human body fluids. Analytical and bioanalytical chemistry, 399(8), 2635-2644.
19. Lämmerhofer, M., Richter, M., Wu, J., Nogueira, R., Bicker, W., & Lindner, W. (2008). Mixed‐mode ion‐exchangers and their comparative chromatographic characterization in reversed‐phase and hydrophilic interaction chromatography elution modes. Journal of separation science, 31(14), 2572-2588.
20. Li, Z., Beeram, S. R., Bi, C., Suresh, D., Zheng, X., & Hage, D. S. (2016). High-performance affinity chromatography: applications in drug–protein binding studies and personalized medicine. In Advances in protein chemistry and structural biology (Vol. 102, pp. 1-39). Academic Press.
21. Matsuda, R., Anguizola, J., Joseph, K. S., & Hage, D. S. (2011). High-performance affinity chromatography and the analysis of drug interactions with modified proteins: binding of gliclazide with glycated human serum albumin. Analytical and bioanalytical chemistry, 401(9), 2811.
22. Mohammadi-Jam, S., & Waters, K. E. (2014). Inverse gas chromatography applications: A review. Advances in colloid and interface science, 212, 21-44.
23. Mun, J. Y., Lee, K. J., Seo, H., Sung, M. S., Cho, Y. S., Lee, S. G., ... & Oh, D. B. (2013). Efficient adhesion-based plasma membrane isolation for cell surface N-glycan analysis. Analytical chemistry, 85(15), 7462-7470.
24. Natarajan, P., Ray, K. K., & Cannon, C. P. (2010). High-density lipoprotein and coronary heart disease: current and future therapies. Journal of the American College of Cardiology, 55(13), 1283-1299.
25. Ostrove, S. (1990). Affinity chromatography: General methods. In Methods in Enzymology (Vol. 182, pp. 357-371). Academic Press.
26. Quanson, J. L., Stander, M. A., Pretorius, E., Jenkinson, C., Taylor, A. E., & Storbeck, K. H. (2016). High-throughput analysis of 19 endogenous androgenic steroids by ultra-performance convergence chromatography tandem mass spectrometry. Journal of Chromatography B, 1031, 131-138.
27. Santos, I. C., & Schug, K. A. (2017). Recent advances and applications of gas chromatography vacuum ultraviolet spectroscopy. Journal of separation science, 40(1), 138-151.
28. Schiel, J. E., & Hage, D. S. (2009). Kinetic studies of biological interactions by affinity chromatography. Journal of separation science, 32(10), 1507-1522.
29. Silva, C. G. A. D.,& Collins, C. H. (2014). Super/subcritical fluid chromatography with packed columns: state of the art and applications. Química Nova, 37(6), 1047-1057.
30. Tarafder, A. (2016). Metamorphosis of supercritical fluid chromatography to SFC: an overview. TrAC Trends in Analytical Chemistry, 81, 3-10.
31. Van den Ouweland, J. M., & Kema, I. P. (2012). The role of liquid chromatography–tandem mass spectrometry in the clinical laboratory. Journal of chromatography B, 883, 18-32.
32. Vlčková, H. K., Pilařová, V., Svobodová, P., Plíšek, J., Švec, F., & Nováková, L. (2018). Current state of bioanalytical chromatography in clinical analysis. Analyst, 143(6), 1305-1325.
33. Wei, F., Hu, Q., Huang, J., Han, S., & Wang, S. (2017). Screening active compounds from Corydalis yanhusuo by combining high expression VEGF receptor HEK293 cell membrane chromatography with HPLC-ESI-IT-TOF-MSn method. Journal of pharmaceutical and biomedical analysis, 136, 134-139.
34. Wilchek, M., & Chaiken, I. (2000). An overview of affinity chromatography. In Affinity Chromatography (pp. 1-6). Humana Press.
35. Xiao-Yu, W., Xiao-Fei, C., Yan-Qiu, G., Yan, C., Yong-Fang, Y., Zhan-Ying, H., & Yi-Feng, C. (2018). Progress of Cell Membrane Chromatography and Its Application in Screening Active Ingredients of Traditional Chinese Medicine. Chinese Journal of Analytical Chemistry, 46(11), 1695-1702.
36. Xie, S. M., & Yuan, L. M. (2017). Recent progress of chiral stationary phases for separation of enantiomers in gas chromatography. Journal of separation science, 40(1), 124-137.
37. Yin, L., Shi, M., Wang, T., Zhang, M., Zhao, X., Zhang, Y., & Gu, J. (2017). A Parallel-Column LC–MS/MS Method for High-Throughput Analysis of Eight Antiepileptic Drugs in Clinical Therapeutic Drug Monitoring. Chromatographia, 80(1), 137-143.
38. Zhang, K., & Liu, X. (2016). Mixed-mode chromatography in pharmaceutical and biopharmaceutical applications. Journal of pharmaceutical and biomedical analysis, 128, 73-88.
39. Zhang, K., Dai, L., & Chetwyn, N. P. (2010). Simultaneous determination of positive and negative pharmaceutical counterions using mixed-mode chromatography coupled with charged aerosol detector. Journal of Chromatography A, 1217(37), 5776-5784.
40. Zhu, M. Z., Wu, W., Jiao, L. L., Yang, P. F., & Guo, M. Q. (2015). Analysis of flavonoids in lotus (Nelumbo nucifera) leaves and their antioxidant activity using macroporous resin chromatography coupled with LC-MS/MS and antioxidant biochemical assays. Molecules, 20(6), 10553-10565.