Determination of fenazaquin in irrigation canal water and soil samples by gas chromatography mass spectrometry

Abstract

The urgent call for the control and regulation of pesticide usage arose from the adverse health effects associated with their excessive and wrong application mostly in the agricultural field. The uncontrolled use of pesticides has been proved to affect the environment because of their ability to accumulate in different environmental resources such as water and soil. Fenazaquin is one of the mostly used pesticides in the agricultural field known by its adverse health effects. In this study, the previously mentioned pesticide "fenazaquin" was analyzed in two different kind of environmental samples: irrigation canal water and soil samples using a highly accurate gas chromatography mass spectrometer (GCMS). In the first step of the study, the limit of qualification (LOD), limit of quantification (LOQ), and relative standard deviation (RSD) were determined and calculated to be 0.04 mg/L, 0.14 mg/L and 10.2%, respectively. In the following step, the samples were spiked at different concentrations using low volumes of high concentration stock solution, to ensure the real effects of the sample matrices were observed. All measurement were performed by applying the adequate temperature program of the sensitive analytical instrument GCMS. The results obtained using the classical recovery procedure was satisfactory but matrix matching calibration strategy was used to improve the percent recoveries to almost 100%. Thus, this study explains the possibility of fenazaquin determination in two different environmental samples using the classical recovery and matrix matching strategies. Irrigation canal water and soil samples are on a high contamination risk from the pesticides used in the agricultural field.

Keywords

• Fenazaquin
• Pesticides
• GC-MS

Gas kromatografi kütle spektrometresi ile sulama kanal suyu ve toprak numunesindeki fenazaquinin tayini

Öz

Pestisitlerin tarımsal alanlarda gereğinden fazla ve yanlış kullanımlarının yol açtığı sağlık problemleri, pestisit kullanımının düzenlenmesi ve kontrol edilmesi için acil müdahalenin gerekliliğini doğurmuştur. Kontrolsüz pestisit kullanımının çevreye olan etkisi su ve toprak gibi çevresel kaynaklarda meydana gelen p ile kanıtlanmıştır. Fenazaquin tarım alanlarında yaygın olarak kullanılan ve canlı ve çevre sağlığı açısından olumsuz etki gösterdiği bilinen pestisitler içerisinde yer almaktadır. Bu çalışmada, iki farklı çevresel kaynaktan alınan numune analiz edilmiş; yüksek hassasiyete sahip bi gaz kromatografisi-kütle spektrometrisi (GC-MS) kullanılarak sulama kanal suyu ve toprak numunelerinde daha önce belirtilen pestisit ‘fenazaquin'in tayini gerçekleştirilmiştir. Çalışmanın ilk aşamasında tespit limiti (LOD), tayin limiti (LOQ) ve bağıl standart sapma (RSD) değerleri sırasıyla 0.04 mg/L, 0.14 mg/L and 10.2% olarak belirlenmiştir.l Bir sonraki adımda, gerçek örnek matrislerinin analiz sonuçlarına etkilerini gözlemlemek amacıyla, yüksek konsantrasyonlu stok çözeltilerin düşük hacimleri ilave edilerek numuneler farklı kokonsantrasyonlarda hazırlanmıştır.Yapılan bütün ölçümlerde hassas analitik cihaz GCMS sisteminde geliştirilen uygun sıcaklık programı kullanılmıştır. Klasik geri kazanım prosedürünü kullanarak elde edilen sonuçlar yeterli derecede olmasına rağmen matris eşleştirme kalibrasyon stratejisinin kullanımı sayesinde geri kazanım oranı neredeyse %100’e yükseltilmiştir. Nitekim, bu çalışma iki farklı çevresel numunedeki fenazaquinin klasik geri kazanım ve matris eşleşme stratejisi ile tespit edilebileceğini açıklamaktadır. Sulama kanalı suyu ve toprak örnekleri tarım alanlarında kullanılan pestisitlerden kaynaklanan yüksek kontaminasyon riski altındadır.

Anahtar Kelimeler

• Fenazaquin
• Pestisitler
• GC-MS

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