A Turn OFF Fluorescent Probe For Selective Detection Of Hg2+ Ions

Abstract

In this study, we prepared a novel fluorescent chemosensor containing an imidazole molecule and the chemosensor characterized utilizing 1H-NMR, 13C-NMR spectroscopy, FT-IR spectrometer and elemental analyzer. Prepared sensor was utilized as an effectively selective and a fastly responsive chemical fluorescent sensor for ‘’turn off’’ determination of mercury (II) ions in EtOH. A clear complex between 2-((4-(1H-phenanthro[9,10-d] imidazol-2-yl)benzylidene)amino) phenol (PENIM) and Hg+ ions was determined and calculated employing the Job’s method and also the limit of detection value was found to be 2.1 nM on the basis of 3σ/k. Furthermore, the sensor-Hg2+ displayed and reversible property for mercapto containing cysteine molecules. Also, the fluorescence enhancement and quenching studies were supported by computational experiments based on the density functional theory (DFT) calculations.

Keywords

• Mercury ions
• Fluorescence
• Sensor

References

1. Chen, G., Guo, Z., Zeng, G., & Tang, L. (2015). Fluorescent and colorimetric sensors for environmental mercury detection. Analyst, 140(16), 5400–5443. https://doi.org/10.1039/c5an00389j
2. Chen, J. F., Han, B. B., Ma, J. F., Liu, X., Yang, Q. Y., Lin, Q., Wei, T. B. (2017). Pillar[5]arene-based fluorescent polymer for selective detection and removal of mercury ions. RSC Advances, 7(75), 47709–47714. https://doi.org/10.1039/c7ra10326c
3. Chen, L., Yang, L., Li, H., Gao, Y., Deng, D., Wu, Y., & Ma, L. J. (2011). Tridentate lysine-based fluorescent sensor for Hg(II) in aqueous solution. Inorganic Chemistry, 50(20), 10028–10032. https://doi.org/10.1021/ic200790g
4. Guo, C., & Irudayaraj, J. (2011). Fluorescent Ag clusters via a protein-directed approach as a Hg(II) ion sensor. Analytical Chemistry, 83(8), 2883–2889. https://doi.org/10.1021/ac1032403
5. Han, B., Yuan, J., & Wang, E. (2009). Sensitive and selective sensor for biothiols in the cell based on the recovered fluorescence of the CdTe quantum dots-Hg(II) system. Analytical Chemistry, 81(13), 5569–5573. https://doi.org/10.1021/ac900769h
6. Jiao, Y., Zhang, L., & Zhou, P. (2016). A rhodamine B-based fluorescent sensor toward highly selective mercury (II) ions detection. Talanta, 150, 14–19. https://doi.org/10.1016/j.talanta.2015.11.065
7. Karuk Elmas, Ş. N., & Yilmaz, I. (2018). A Turn off-on Fluorescent Chemosensor for Sequential Determination of Mercury and Biothiols. Journal of Fluorescence, 28(6), 1451–1458. https://doi.org/10.1007/s10895-018-2320-6
8. Li, G., Ma, L., Liu, G., Fan, C., & Pu, S. (2017). A diarylethene-based “on-off-on” fluorescence sensor for the sequential recognition of mercury and cysteine. RSC Advances, 7(33), 20591–20596. https://doi.org/10.1039/c6ra27773j

9. Li, M., Zhou, X., Ding, W., Guo, S., & Wu, N. (2013). Fluorescent aptamer-functionalized graphene oxide biosensor for label-free detection of mercury(II). Biosensors and Bioelectronics, 41(1), 889–893. https://doi.org/10.1016/j.bios.2012.09.060
10. Li, Q., Wang, C., Tan, H., Tang, G., Gao, J., & Chen, C. H. (2016). A turn on fluorescent sensor based on lanthanide coordination polymer nanoparticles for the detection of mercury(II) in biological fluids. RSC Advances, 6(22), 17811–17817. https://doi.org/10.1039/c5ra26849d
11. Taki, M., Akaoka, K., Iyoshi, S., & Yamamoto, Y. (2012). Rosamine-based fluorescent sensor with femtomolar affinity for the reversible detection of a mercury ion. Inorganic Chemistry, 51(24), 13075–13077. https://doi.org/10.1021/ic301822r.
12. Lin W., Long L., Yuan L., Cao Z., Chen B., and Tan W., (2008). A Ratiometric Fluorescent Probe for Cysteine and Homocysteine Displaying a Large Emission Shift, Org. Lett., 10(24), 5577-5580. https://doi.org/10.1021/ol802436j.