İstanbul İli Avrupa Yakası Bazı Bölgelerinde Hava Kalitesinin Mikrobiyolojik Yoğunluk Açısından İncelenmesi

Öz

İstanbul’un dış havasının mikrobiyal faunası avrupa yakasına ait dört faklı bölgede (Bakırköy, Fatih, Başakşehir ve Esenler) aktif ve pasif sedimentasyon örnekleme metoduyla incelenmiştir. Meteorolojik faktörlere ilave olarak, kentleşme ve trafik yoğunluğu mikrobiyal yükleme için tespit edilmiştir. Dış hava örnekleme Nisan 2014-Kasım 2015 arasında yürütülmüştür. Sedimentasyon yöntemi standart plate count agar (PCA) kullanılarak hazırlanan petri kutularının kapakları açılarak yapılmıştır. PCA ortamı toplam bakterilerin belirlenmesinde kullanılırken mantarlar için malt ekstrakt agar (MEA) kullanılmıştır. Bu periyodun sonunda oluşan koloniler sayılmış ve hacim başına düşen toplam bakteri ve mantar sayısı belirlenmiştir. Hava sıcaklığı, bağıl nem, rüzgar hızı ve yönü de aynı zamanda kayıt edilmiştir. Aktif örnekleme metodu sonuçlarına göre, en fazla bakteri sayısı 350C inkübasyon sıcaklığında Başaksehir istasyonunda 222 KOB (Koloni oluşturan birey)/m3, en fazla mantar sayısı 200C inkübasyon sıcaklığında da 286 KOB/m3 olarak belirlenmiştir. Belirlenen mikroorganizmalar için herhangi bir tür teşhis yapılmamıştır. Pasif örnekleme metodunda en fazla bakteri sayımı sonucu 350C ‘da Bakırkoy istasyonunda 9250 KOB/m3 olarak saptanmıştır.

Anahtar Kelimeler

• Mikrobiyal fauna,dış ortam havası,sedimentasyon örnekleme,mikrobiyal yük

Investigation of Air Quality in Terms of Microbiological Density in Some Regions of the European Side of Istanbul Province

Abstract

Microbial fauna of the outdoor ambient air of Istanbul has been determined in European side of Istanbul province at four different districts (Bakirkoy, Fatih, Basaksehir and Esenler) by active and passive sedimentation sampling methods. In addition to meteorological factors, the effect of urbanization and traffic density for the air microbial load is also determined. Outdoor air sampling was performed between April 2014 and November 2015. The sedimentation method was done by standart plate count agar (PCA) opening the cover of the petri dishes containing a prepared medium. Standart plate count agar (PCA) medium was used for the determination of total bacteria while malt extract agar (MEA) was used for fungi. The colonies formed at the end of this period were counted and total number of bacteria and fungi per unit volume and area were determined. Air temperature, relative humidity, wind speed and direction were also recorded. As a result of the active sampling method, the most counted units of bacteria was determined at 350C in Basaksehir station as 222 CFU(Colony forming unit)/m3, maximum fungal was determined as 286 CFU/m3 at 200C incubation temperature. The maximum amount of bacteria in the passive sampling method results at 350C was in Bakirkoy station as 9250 CFU/m3.

Keywords

• Microbial fauna,outdoor air,sedimentation sampling,microbial load

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