Isolation Entomopathogenic fungi From The Two-Spotted Spider Mite (Teranychus urticae) at Erzincan Provience

Abstract

This study was conducted between 2015-2017. The infested with mites of the bean leaves were collected from the field. Then put in polyethylene bags, and brought to the laboratory. The mites on the bean leaves were transferred to the petri dish with Potato Dexrose Aagar (PDA) by a brush at the laboratory. The petri dishes were incubated for growth and sporulation of the fungus (2 weeks in the incubator at 25±1ºC). As a result, six entomopathogenic fungi (EPF) ısolates were isolated from the cadaver of the Tetranychus urticae Koch, 1836(Acari: Tetranychidae) Four of them were Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes: Hyphomycetes) and two of them were Metharizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes). After the identification of isolates, they have been coded and stored under refrigerator conditions for use in other studies at the Pharmaceutical Microbiology Laboratory of the Faculty of Pharmacy, Erzincan Binali Yıldırım University.

Keywords

• Beauveria bassiana
• Metharizium anisopliae
• Tetranychus urticae
• Erzincan

Erzincan İlinde İki Noktalı Kırmızı Örümcek [Tetranychus urticae Koch, 1836 (Acari: Tetranychidae)]’den Entomopatojenik Fungus İzolasyonu

Abstract

Bu çalışma 2015-2017 yılları arasında yürütülmüştür. Kuru fasulye üretim alanlarından akarlarla bulaşık fasulye yaprakları toplanıp, polietilen torbabalara konularak laboratuvara getirilmiştir. Laboratuvarda bir fırça yardımıyla fasulye yapraklarındaki akarlar Patates Dekstroz Aagarlı (PDA) petrilere aktarılmıştır. Petriler fungusların gelişimi ve sporulasyonu için inkübatöre konulmuştur (2 hafta-25±1ºC). Tetranychus urticae Koch, 1836 (Acari:Tetranychidae) kadavralarından 6 adet entomopatojenik fungus (EPF) izolatı elde edilmiştir. Bunlardan 4’ü Beauveria bassiana (Balsamo) Vuillemin (Deuteromycetes: Hyphomycetes) ve 2’si Metharizium anisopliae (Metsch.) Sorokin (Deuteromycotina: Hyphomycetes) olduğu teşhis edilmiştir. Teşhisten sonra izolatlar kodlanmış ve daha sonraki çalışmalar için Erzincan Binali Yıldırım Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Laboratuvarında buzdolabı şartlarında depolanmıştır.

Keywords

• Beauveria bassiana
• Metharizium anisopliae
• Tetranychus urticae
• Erzincan

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