Year 2020, Volume , Issue 18, Pages 879 - 884 2020-04-15

Treatment of fizzy drink factory wastewaters by microalgae and evaluation of algae oil for biofuel production

Togayhan KUTLUK [1] , Nurcan KAPUCU [2]

As a result of city, industrial and agricultural activities, a large amount of wastewater is generated every year. The wastewater generated must be treated. When the nitrogen concentration in wastewater reaches more than 1.9 mg / L, favorable environmental conditions are provided for the microalgae to multiply. In the presence of microalgae in wastewater treatment technologies, treatment has gained importance in recent years and has become a more environmentally-friendly alternative in treatment. Research on the availability of biomass as a source of raw materials in the production of alternative energy sources is increasingly important. In this paper, Chlorella variabilis microalgae growth, lipid productivity and nutrient removal in wastewater media were investigated. Before inoculation of microalgae, wastewater was centrifuged at 4000 rpm to remove the solid particles and was diluted with tap water in different ratios (0-40%). Wastewaters were inoculated with Chlorella variabilis in 250 mL open flasks in a 200 rpm shaking incubator for a month at 27 °C. After incubation maximum cell concentration (Xmax=1.03 gdw/L), growth rate (µmax=4.0 x10-3), doubling time (173 h) of the microalgae were reached in 40% diluted medium. Fat content (21%) and lipid productivity (6x10-3 g/L.d) were determined concurrently for 20% diluted media. It was determined that microalgae lipids were rich in oleic (C18:1 38%) and linolenic acid (C18:3 35%). The efficiency of COD (Chemical oxygen demand) and total phosphor removal in the presence of microalgae had been almost 60% and 77% respectively. According to the experimental results, the treatment of wastewater in the presence of microalgae is promising for future applications.
wastewater, microalgae, fizzy drink
  • [1] Girard, J-M., Roy, M-L., Hafsa, B.M., Gagnon, J., Faucheux, N., Heitz, M., Tremblay, R., Deschenes, J-S. (2014). Mixotrophic cultivation of green microalgae Scenedesmus obliquus on cheese whey permeate for biodiesel production, Algal Research, 5, 241–248.
  • [2] Li, C., Yang, H., Xia, X., Li, Y., Chen, L., Zhang, M., Zhang, M., Wang, W. (2013). High efficient treatment of citric acid effluent by Chlorella vulgaris and potential biomass utilization, Bioresource Technology 127, 248–255.
  • [3] Abinandan, S., Shanthakumar, S. (2015). Challenges and opportunities in application of microalgae (ChloropHyta) for wastewater treatment: A review, Renewable and Sustainable Energy Reviews, 52, 123–132.
  • [4] Wu, L.F., Chen, P.C., Huang, A.P., Lee, C.M. (2012). The feasibility of biodiesel production by microalgae using industrial wastewater, Bioresource Technology, 113,14-18.
  • [5] Hongyang, S., Yalei, Z., Chunmin, Z., Xuefei, Z., Jinpeng, L. (2011), Cultivation of Chlorella pyrenoidosa in soybean processing wastewater, Bioresource Technology, 102, 21, 9884-9890.
  • [6] Bligh, E.G., Dyer, W.J. (1959). A rapid method for total lipid extraction and purification, Canadian Journal of Biochemistry and Physiology, 37: 911-917.
  • [7] David, F, Sandra P, Vickers, A.K. (2005). Column selection for the analysis of fatty acids methyl esters. Application Agilent Technolojies Inc.
  • [8] Magri, A., Beline, F., Dabert, F. (2013). Feasibility and interest of the anammox process as the treatment alternative for anaerobic digester supernatants in manure processing: An overview, Journal of Environmental Management, 131, 170-184.
  • [9] Levine, R. B., Costanza-Robinson, M.S., Spatafora, G.A. (2011). Neochloris oleoabundans grown on anaerobically digested dairy manure for concomitant nutrient removal and biodiesel feedstock production, Biomass and Bioenergy, 351, 40-49.
  • [10] Klopfenstein, W.E. (1985). Effect of molecular weights of fatty acid esters on cetane numbers as diesel fuel, Journal of American Oil Chemical Socitey, 62, 1029– 1031.
  • [11] Gopinath, A, Puhan, S, Nagarajan, G. (2009). Relating the cetane number of biodiesel fuels to their fatty acid composition: A critical study, Proceedings of the Institution of Mechanical Engineers, Part D: Journal of Automobile Engineering, 223, 565-583.
Primary Language en
Subjects Engineering
Journal Section Articles

Orcid: 0000-0001-7249-9902
Author: Togayhan KUTLUK
Country: Turkey

Orcid: 0000-0002-5542-7816
Author: Nurcan KAPUCU (Primary Author)
Country: Turkey


Publication Date : April 15, 2020

APA Kutluk, T , Kapucu, N . (2020). Treatment of fizzy drink factory wastewaters by microalgae and evaluation of algae oil for biofuel production . Avrupa Bilim ve Teknoloji Dergisi , (18) , 879-884 . DOI: 10.31590/ejosat.684478