Although traditional microbial methods based on microscopy and culture techniques are highly effective, they are sometimes inadequate to identify the high diversity of microbial species found in environmental samples. In the past two decades, molecular techniques have improved significantly, and genomic approaches have been used to provide a more comprehensive and quantitative description of the distribution of microorganisms. In this pilot study, prokaryotic and eukaryotic microbial diversity of the samples from Lake Tuz was investigated by metabarcoding approach. According to the 16S / 18S rDNA sequencing results, an average of 29 Archaea, 23 Bacteria and 61 Eukaryotic OTUs were determined in the samples and the ratio of prokaryotic OTUs was 65.3%. In all examples, the most detected archaeal OTU belongs to Haloquadratum walsbyi from the Euryarchaeota branch, and the most common bacterial OTUs belong to the members of the genus Salinibacter. In accordance with the 18S rDNA sequencing results, the most abundant eukaryotic OTU is Dunaliella salina. In this study, many prokaryotic and eukaryotic OTUs that could not be cultured in vitro were detected and an OTU with less than 97% similarity ( 92%) to 16S rDNA sequences in their databases was determined. The results obtained have the potential to contribute to the clarification of the structure and composition of microbial communities in Lake Tuz.
|Supporting Institution||Hacettepe Üniversitesi|
|Thanks||Çalışmanın planlanması sırasındaki yardımcı ve eleştirel yorumları için Prof. Dr. Hatice Mergen, Doç. Dr. Sırma Çapar Dinçer ve Deniz Eyice’ye teşekkür ederim. Bu çalışma Hacettepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi (FHD-2017-16050 numaralı proje) tarafından desteklenmiştir.|
Publication Date : August 31, 2020
|APA||Kucukyi̇ldi̇ri̇m Celi̇k, S , Ünal, H . (2020). Metabarkodlama yaklaşımıyla Tuz Gölü, Türkiye mikroorganizmalarının belirlenmesi için bir pilot çalışma . Avrupa Bilim ve Teknoloji Dergisi , (19) , 366-374 . DOI: 10.31590/ejosat.682557|