Punikalaginin Kolistin Dirençli Acinetobacter baumannii Suşlarına Karşı Tek Başına ve Kolistin ile Kombinasyon Halinde Antimikrobiyal Aktivitesinin Değerlendirilmesi

Year 2021, Issue: 32, 670 - 674, 31.12.2021

Elif Odabaş Köse

https://doi.org/10.31590/ejosat.1041147

Abstract

Bu çalışmanın amacı, punikalaginin tek başına ve kolistin ile kombinasyon halinde antimikrobiyal aktivitesini kolistine dirençli A. baumannii suşları üzerinde değerlendirmektir. Çalışmada test edilmek üzere beş adet kolistine dirençli A. baumannii suşu kullanılmıştır. Suşlara karşı punikalagin ve kolistinin antimikrobiyal aktivitelerini saptamak için sıvı mikrodilüsyon testi yapılarak Minimum İnhibisyon Konsantrasyonları (MİK) belirlenmiştir. Bu iki ajanın kombinasyon aktiviteleri ise Dama Tahtası (Checkerboard) Sinerji Testi ile değerlendirilmiştir. Sıvı mikrodilüsyon testinin sonuçlarına göre tüm suşlara karşı punikalaginin MİK değeri 256 µg/ml, kolistinin MİK değerleri ise iki suşta 32 µg/ml, diğer üç suşta 8 µg/ml olarak tespit edilmiştir. Checkerboard sinerji testi ile kombinasyon etkileşimlerinin belirlenmesinde Fraksiyonel İnhibitör Konsantrasyon İndeksi (FİKİ) hesaplanmıştır. Buna göre kolistin ve punikalagin kombinasyonunun FİKİ değerleri iki suş için 1.12, diğer üç suş için ise 1.03 olarak bulunmuştur. Sonuç olarak, punikalagin kolistin dirençli A. baumannii suşları üzerinde antimikrobiyal aktivite göstermiştir. Kolistin ile kombinasyonunun ise tüm test edilen suşlar üzerinde indiferan etkili olduğu tespit edilmiştir. Hiçbir suş üzerinde sinerjistik etki gözlenmemiştir. Bunun yanısıra herhangi bir antagonistik etki de saptanmamıştır.

Keywords

Acinetobacter baumannii, kolistin, punicalagin, indiferan etki

Evaluation of the Antimicrobial Activity of Punicalagin Alone and in Combination with Colistin Against Colistin-Resistant Acinetobacter baumannii Strains

Abstract

The aim of this study was to evaluate the antimicrobial activity of punicalagin alone and in combination with colistin on colistin-resistant A. baumannii strains. Five colistin-resistant A. baumannii strains were used to be tested in the study. Broth microdilution test was performed to evaluate the antimicrobial activities of punicalagin and colistin against strains and Minimum Inhibition Concentrations (MIC) were determined. Combination activities of these two agents were evaluated with the Checkerboard Synergy Test. According to the results of the broth microdilution test, the MIC value of punicalagin against all strains was 256 µg/ml, the MIC values of colistin were found to be 32 µg/ml on two strains and 8 µg/ml on the other three strains. The Fractional Inhibitory Concentration Index (FICI) was calculated to determine combination interactions with the Checkerboard synergy test. Accordingly, the FICI values of the combination of colistin and punicalagin were found as 1.12 for two strains, 1.03 for the other three strains. In conclusion, punicalagin showed antimicrobial activity on colistin-resistant A. baumannii strains. It was determined that the combination with colistin had an indifferent effect on all tested strains. No synergistic effects were observed on any of the strains. In addition, no antagonistic effects were detected.

Keywords

Acinetobacter baumannii, colistin, punicalagin, indifferent effect

References

• Álvarez-Martínez, F. J., Rodríguez, J. C., Borrás-Rocher, F., Barrajón-Catalán, E., & Micol, V. (2021). The antimicrobial capacity of Cistus salviifolius and Punica granatum plant extracts against clinical pathogens is related to their polyphenolic composition. Scientific reports, 11(1), 1-12.
• Al-Zoreky, N. S. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International journal of food microbiology, 134(3), 244-248.
• Aqil, F., Munagala, R., Vadhanam, M. V., Kausar, H., Jeyabalan, J., Schultz, D. J., & Gupta, R. C. (2012). Anti-proliferative activity and protection against oxidative DNA damage by punicalagin isolated from pomegranate husk. Food Research International, 49(1), 345-353.
• Asif, M., Alvi, I. A., & Rehman, S. U. (2018). Insight into Acinetobacter baumannii: pathogenesis, global resistance, mechanisms of resistance, treatment options, and alternative modalities. Infection and drug resistance, 11, 1249.
• Ayoub Moubareck, C., & Hammoudi Halat, D. (2020). Insights into Acinetobacter baumannii: a review of microbiological, virulence, and resistance traits in a threatening nosocomial pathogen. Antibiotics, 9(3), 119.
• Berdowska, I., Matusiewicz, M., & Fecka, I. (2021). Punicalagin in Cancer Prevention—Via Signaling Pathways Targeting. Nutrients, 13(8), 2733.
• Celiksoy, V., & Heard, C. M. (2021). Antimicrobial Potential of Pomegranate Extracts. In Pomegranate. IntechOpen.
• Clinical and Laboratory Standards Institute (CLSI). 2018. Performance Standards for Antimicrobial Susceptibility Testing. 28th ed. CLSI Supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute.
• da Silva, R. A., Ishikiriama, B. L. C., Ribeiro Lopes, M. M., de Castro, R. D., Garcia, C. R., Porto, V. C., ... & Lara, V. S. (2020). Antifungal activity of Punicalagin–nystatin combinations against Candida albicans. Oral Diseases, 26(8), 1810-1819.
• Du, R., Cooper, L., Chen, Z., Lee, H., Rong, L., & Cui, Q. (2021). Discovery of chebulagic acid and punicalagin as novel allosteric inhibitors of SARS-CoV-2 3CLpro. Antiviral research, 190, 105075.
• Duman, A. D., Ozgen, M., Dayisoylu, K. S., Erbil, N., & Durgac, C. (2009). Antimicrobial activity of six pomegranate (Punica granatum L.) varieties and their relation to some of their pomological and phytonutrient characteristics. Molecules, 14(5), 1808-1817.
• Endo, E. H., Cortez, D. A. G., Ueda-Nakamura, T., Nakamura, C. V., & Dias Filho, B. P. (2010). Potent antifungal activity of extracts and pure compound isolated from pomegranate peels and synergism with fluconazole against Candida albicans. Research in Microbiology, 161(7), 534-540.
• Ganesan, T., Sinniah, A., Chik, Z., & Alshawsh, M. A. (2020). Punicalagin regulates apoptosis-autophagy switch via modulation of annexin A1 in colorectal cancer. Nutrients, 12(8), 2430.
• Gosset-Erard, C., Zhao, M., Lordel-Madeleine, S., & Ennahar, S. (2021). Identification of punicalagin as the bioactive compound behind the antimicrobial activity of pomegranate (Punica granatum L.) peels. Food Chemistry, 352, 129396.
• Houston, D. M., Bugert, J. J., Denyer, S. P., & Heard, C. M. (2017). Potentiated virucidal activity of pomegranate rind extract (PRE) and punicalagin against Herpes simplex virus (HSV) when co-administered with zinc (II) ions, and antiviral activity of PRE against HSV and aciclovir-resistant HSV. PloS one, 12(6), e0179291.
• Howard, A., O’Donoghue, M., Feeney, A., & Sleator, R. D. (2012). Acinetobacter baumannii: an emerging opportunistic pathogen. Virulence, 3(3), 243-250.
• Li, G., Yan, C., Xu, Y., Feng, Y., Wu, Q., Lv, X., ... & Xia, X. (2014). Punicalagin inhibits Salmonella virulence factors and has anti-quorum-sensing potential. Applied and environmental microbiology, 80(19), 6204-6211.
• Li, J., Fu, Y., Zhang, J., Zhao, Y., Fan, X., Yu, L., ... & Li, C. (2020). The efficacy of colistin monotherapy versus combination therapy with other antimicrobials against carbapenem-resistant Acinetobacter baumannii ST2 isolates. Journal of Chemotherapy, 32(7), 359-367.
• Machado, T. D. B., Leal, I. C., Amaral, A. C. F., Santos, K., Silva, M. G. D., & Kuster, R. M. (2002). Antimicrobial ellagitannin of Punica granatum fruits. Journal of the Brazilian Chemical Society, 13(5), 606-610.
• Moody J. Synergism testing: broth microdilution checkerboard and broth macrodilution methods. In: Isenberg HD (Ed.) Clinical Microbiology Procedures Handbook, 2nd ed. Washington, DC: ASM Press, 2007: p.5.12.1-5.12.23.
• Mun, S. H., Kang, O. H., Kong, R., Zhou, T., Kim, S. A., Shin, D. W., & Kwon, D. Y. (2018). Punicalagin suppresses methicillin resistance of Staphylococcus aureus to oxacillin. Journal of pharmacological sciences, 137(4), 317-323.
• Qureshi, Z. A., Hittle, L. E., O'Hara, J. A., Rivera, J. I., Syed, A., Shields, R. K., ... & Doi, Y. (2015). Colistin-resistant Acinetobacter baumannii: beyond carbapenem resistance. Clinical infectious diseases, 60(9), 1295-1303.
• Rodjun, V., Houngsaitong, J., Montakantikul, P., Paiboonvong, T., Khuntayaporn, P., Yanyongchaikit, P., & Sriyant, P. (2020). In Vitro Activities of Colistin and Sitafloxacin Combinations against Multidrug-, Carbapenem-, and Colistin-Resistant Acinetobacter baumannii Using the Broth Microdilution Checkerboard and Time-Kill Methods. Antibiotics, 9(8), 516.
• Seeram, N. P., Adams, L. S., Henning, S. M., Niu, Y., Zhang, Y., Nair, M. G., & Heber, D. (2005). In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. The Journal of nutritional biochemistry, 16(6), 360-367.
• Trabelsi, A., El Kaibi, M. A., Abbassi, A., Horchani, A., Chekir-Ghedira, L., & Ghedira, K. (2020). Phytochemical study and antibacterial and antibiotic modulation activity of Punica granatum (Pomegranate) leaves. Scientifica, 2020.
• Xu, Y., Shi, C., Wu, Q., Zheng, Z., Liu, P., Li, G., ... & Xia, X. (2017). Antimicrobial activity of punicalagin against Staphylococcus aureus and its effect on biofilm formation. Foodborne pathogens and disease, 14(5), 282-287.