Profile of fatty acids, vitamins, phytosterols and phenolic acids in Trachystemon orientalis L. and evaluation of their antioxidant activity

Year 2022, Issue: 33, 112 - 118, 31.01.2022

Ersin Demir

https://doi.org/10.31590/ejosat.1027061

Cited By: 1

Abstract

The present study focuses on the phytochemical content and antiradical properties of Trachystemon orientalis L., which grows spontaneously and is consumed as food in rural areas of Düzce (from Turkey) province. DPPH, ABTS and OH radical scavenging tests were utilized for identifying the antiradical properties of the extracts of this plant. In addition, the metal chelating potential of this plant was also evaluated. The higher the inhibition % value calculated in these tests, the higher the antioxidant activity was considered and the results were evaluated. T. orientalis the average ABTS radical cleaning test results of extracts prepared in different concentrations of methanol, ethanol and purified water were found to be 93.35%, 91.32%, 94.70%, respectively. The average DPPH radical cleaning test results of the extracts of this plant prepared in different concentrations of methanol, ethanol and purified water were determined as 37.26%, 7.95%, 52.26%, respectively. T. orientalis the OH radical cleaning test results of the extracts of plant prepared from methanol, ethanol and purified water were determined as 60.85%, 61.33%, 24.12%, respectively. In addition, the metal chelation test results of methanol, ethanol and pure water extracts of this plant were found to be 63.57%, 42.04%, 82.64%. It was determined that the highest content of protein (20.93 mgBSA/g), proanthocyanidin (13.76 µg CE/g) and phenolic (3621.03 µg GAE/g) was found in T. orientalis pure water extract. It has been determined that gallic acid (187.67 μg/g), vanillic acid (3.78 μg/g), rosmarinic acid (10.55 μg/g) and hydrocynamic acid (0.78 μg/g) are found in different proportions in T. orientalis plant. It has been observed that T. orientalis is an important source of palmitic acid, stearic acid, oleic acid, linoleic acid, γ-linolenic acid, alpha-linolenic acid, gadoleic acid and docosahexaenoic acid. It has been established that this plant has a low content of fat-soluble vitamins and phytosterols.

Keywords

edible plants,, antioxidant, fatty acid composition, vitamins, phenolic acids, phytosterols

Supporting Institution

YOK

Project Number

YOk

Thanks

I wish to thank Prof. Dr. Ökkeş Yılmaz for their contributions.

Trachystemon orientalis L.'in yağ asitleri, vitaminler, fitosteroller ve fenolik asitlerin profili ve antioksidan aktivitelerinin değerlendirilmesi

Abstract

Bu çalışmada Düzce ilinin kırsal yerlerinde kendiliğinden yetişen ve gıda olarak tüketilen Trachystemon orientalis L.’nin fitokimyasal içeriği ile antiradikal özellikleri araştırılmıştır. Bu bitkinin özütlerinin antiradikal özellikleri DPPH, ABTS ve OH radikal temizleme testleri kullanılarak belirlendi. Ayrıca bu bitkinin metal şelatlama potansiyeli de değerlendirildi. Bu testlerde hesaplanan % inhibisyon değeri ne kadar yüksekse antioksidan aktivite o kadar yüksek kabul edilerek sonuçlar değerlendirilmiştir. T. orientalis’in farklı konsantrasyonlarda metanol, etanol ve saf suda hazırlanan özütlerinin ortalama ABTS radikal temizleme test sonuçları sırasıyla %93,35, %91,32, %94,70 olarak tespit edilmiştir. Bu bitkinin farklı konsantrasyonlarda metanol, etanol ve saf suda hazırlanan özütlerinin ortalama DPPH radikal temizleme test sonuçları sırasıyla %37,26, %7,95, %52,26 olarak belirlenmiştir. T. orientalis bitkisinin metanol, etanol ve saf suda hazırlanan özütlerinin OH radikal temizleme test sonuçları sırasıyla %60,85, %61,33, %24,12 olarak tespit edilmiştir. Ayrıca bu bitkinin metanol, etanol ve saf suda hazırlanan özütlerinin metal şelatlama test sonuçları % 63,57, %42,04, %82,64 olarak saptanmıştır. T. orientalis saf su özütünde protein (20,93 mgBSA/g), proantosiyanidin (13.76 μg CE/g) ve fenolik (3621.03 μg GAE/g) içeriğinin en yüksek olduğu belirlenmiştir. T. orientalis bitkisinde gallik asit (187.67 μg/g), vanillik asit (3.78 μg/g), rosmarinik asit (10.55 μg/g) ve hidrosinamik asidin (0.78 μg/g) farklı oranlarda bulunduğu belirlenmiştir. T.orientalis bitkisinin palmitic acid, stearic acid, oleic acid, linoleic acid, γ-linolenic acid, alpha-linolenic acid, gadoleic acid ve docosahexaenoic acid bakımından önemli kaynak olduğu görülmüştür. Bu bitkinin yağda çözünebilen vitamin ve fitosterol içeriğinin düşük olduğu saptanmıştır.

Keywords

yenilebilir bitkiler, antioksidan, yağ asidi bileşimi, ,vitaminler, ,fenolik asitler

Project Number

YOk

References

• Amaeze OU, Ayoola GA, Sofidiya MO, Adepoju-Bello AA, Adegoke AO, Coker HA. Evaluation of antioxidant activity of Tetracarpidium conophorum (Müll. Arg) Hutch & Dalziel leaves. Oxid Med Cell Longev. 2011; 2011:976701. doi: 10.1155/2011/976701.
• Ayhan BS, Yalçın E, Çavuşoğlu K, Acar A. Antidiabetic potential and multi‑biological activities of Trachystemon orientalis extracts. Journal of Food Measurement and Characterization 2019; 13:2887–2893.
• Ayvaz Col M. Antioxidant activity of Trachystemon orientalis (L.) G. Don (Borage) grown and eaten as food in Ordu, Turkey. Herba Pol 2015; 61(4): 40-51.
• Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe SE. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2):329-54. doi: 10.1152/physrev.00040.2012.
• Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology 1995; 28: 25–30. doi.org/10.1016/S0023-6438( 95)80008 -5
• Christie WW. (1990). Gas chromatography and lipids. (1th ed.). Scotland: The Oily Pres.
• Christie WW. Preparation of fatty acid methyl esters. Inform, 1992; 3: 1031–1034.
• Decker EA, Welch B. Role of ferritin as a lipid oxidation catalyst in muscle food”, Journal of Agricultural and Food Chemistry, 1990; 38: 674-677.
• Demir E, Turfan N, Özer H, Üstün NŞ, Pekşen A. Nutrient and bioactive substance contents of edible plants grown naturally in Salıpazarı (Samsun). Acta Sci. Pol. Hortorum Cultus, 2020, 19(1), 151–160. doi: 10.24326/asphc.2020.1.14.
• Dimcheva V, Kaloyanov N, Karsheva M (2019). The polyphenol composition of Cistus incanus L., Trachystemon orientalis L. and Melissa officinalis L. infusions by HPLC-DAD method. Open J Anal Bioanal Chem 3(1): 031-038. DOI: http://dx.doi.org/10.17352/ojabc.000008.
• Halliwell B, Gutteridge JM, Aruoma OI. The deoxyribose method: a simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals. Anal Biochem. 1987; 165(1):215-9. doi: 10.1016/0003-2697(87)90222-3.
• Hara A, Radin NS. Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem. 1978; 90(1):420-6. doi: 10.1016/0003-2697(78)90046-5.
• Hussain T, Tan B, Yin Y, Blachier F, Tossou MC, Rahu N. Oxidative stress and inflammation: What polyphenols can do for us? Oxid Med Cell Longev. 2016; 2016:7432797. doi: 10.1155/2016/7432797.
• Keser S, Demir E, Yilmaz Ö. Phytochemicals and antioxidant activity of the almond kernel (Prunus dulcis Mill.) from Turkey. J. Chem. Soc. Pak 2014; 36: 534-541.
• Kibar B, Kibar H. Determination of the nutritional and seed properties of some wild edible plants consumed as vegetable in the Middle Black Sea Region of Turkey. South African Journal of Botany 2017; 108:117–125.
• López-Cervantes J, Sánchez-Machado DI, Ríos-Vázquez NJ. High-performance liquid chromatography method for the simultaneous quantification of retinol, alpha-tocopherol, and cholesterol in shrimp waste hydrolysate. J Chromatogr A. 2006; 1105(1-2):135-9. doi: 10.1016/j.chroma.2005.08.010.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75.
• Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv., 2015, 5, 27986-28006. doi: 10.1039/c4ra13315c.
• Özbakır Özer M, Aksoy M. Mineral composition and nutritional properties of Trachystemon orientalis (L.) G. Don populations in the central black sea region of Turkey. Acta Sci. Pol. Hortorum Cultus, 2019; 18(4):157–167. doi: 10.24326/asphc.2019.4.15.
• Özcan T. Analysis of the total oil and fatty acid composition of seeds of some Boraginaceae taxa from Turkey. Plant Syst Evol 2008 274:143–153. doi 10.1007/s00606-008-0039-6
• Özen T. Antioxidant activity of wild edible plants in the Black Sea Region of Turkey. Grasas Y Aceites 2010; 61(1): 86-94.
• Özer, M.Ö., Aksoy, M. Mineral composition and nutritional properties of Trachystemon orientalis (L.) G. Don populations in the Central Black Sea Region of Turkey. Acta Sci. Pol. Hortorum Cultus (2019; 18(4): 157–167. doi: 10.24326/asphc.2019.4.15.
• Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative Stress: Harms and benefits for human health. Oxid Med Cell Longev. 2017; 2017:8416763. doi: 10.1155/2017/8416763.
• Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231-7. doi: 10.1016/s0891-5849(98)00315-3.
• Roe M, Church S, Pinchen H, Finglas P. Nutrient analysis of fruit and vegetables. Analytical Report. Institute of Food Research, Norwich Research Park, Colney, Norwich. 2013.
• Sacan O. Antioxidant Activity, Total Phenol and Total Flavonoid Contents of Trachystemon orientalis (L.) G. Don. Eur J Biol 2018; 77(2): 70-75.
• Sánchez-Machado DI, López-Hernández J, Paseiro-Losada P. High-performance liquid chromatographic determination of alpha-tocopherol in macroalgae. J Chromatogr A. 2002; 976(1-2):277-84. doi: 10.1016/s0021-9673(02)00934-2.
• Slavin JL, Lloyd B. Health benefits of fruits and vegetables. Adv Nutr. 2012;3(4):506-16. doi: 10.3945/an.112.002154. Slinkard K, Singleton VL. Total phenol analysis-automation and comparison with manual methods. American Journal of Enology and Viticulture, 1977; 28: 49–55.
• Sun L, Zhang J, Lu X, Zhang L, Zhang Y. Evaluation to the antioxidant activity of total flavonoids extract from persimmon (Diospyros kaki L.) leaves. Food Chem Toxicol. 2011; 49(10):2689-96. doi: 10.1016/j.fct.2011.07.042.
• Woo Y, Lee H, Jeong YS, Shin GY, Oh JG, Kim JS, Oh J. Antioxidant potential of selected Korean edible plant extracts. Biomed Res Int. 2017; 2017: 7695605. doi: 10.1155/2017/7695605.
• Zu Y, Li C, Fu Y, Zhao C. Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. J Pharm Biomed Anal. 2006; 41(3):714-9. doi: 10.1016/j.jpba.2005.04.052.