Determination of TNF-beta Marker in Intrauterine E. coli Induced Endometritis Model in Rats

Abstract

Objectives: This study aims to investigate the importance of tumor necrosis factor (TNF)-beta marker in the endometritis model created by E. coli.

Materials and Methods: Rats were divided into control and endometritis groups with 7 rats in each group in the study. All animals were fed as ad libitum. No application was made to rats in the control group. Progesterone (P4) was administered subcutaneously to rats in the experimental group at a daily dose of 16 mg/kg between the 0th and 4th days of the study. E. coli was administered intrauterinely at 25 μl and 1 × 105 CFU/rat on the 3rd day. Blood samples were taken at the end of the study after the lives of all animals were terminated in accordance with ethical rules. Hemogram analysis was performed for white blood cell (WBC) levels from the blood samples taken. The TNF-beta analysis was performed with a commercial kit by separating serum from blood samples.

Results: WBC (p=0.0004) and TNF-beta (p=0.027) were statistically higher in the endometritis group compared to the control group.

Conclusion: Consequently, increased TNF-beta in endometritis may be a diagnostic marker for inflammations in the genital tract. However, its effectiveness may be better demonstrated by conducting comprehensive studies with the relevant marker.

Keywords

• TNF-beta
• WBC
• Endometritis
• Rat

Abstract

Amaç: Çalışmanın amacı, E. coli ile oluşturulan endometritis modelinde tümör nekroz faktör (TNF) -beta markırının öneminin araştırılması amaçlandı.

Gereç ve Yöntem: Çalışmada ratlar her grupta 7’şer rat olacak şekilde kontrol ve endometritis grubuna ayrıldı. Tüm hayvanlar ad libitum olarak beslendi. Kontrol gruplarına herhangi bir uygulama yapılmamıştır. Deneysel gruba ise Çalışmanın 0 ve 4. günleri arasında ratlara günlük subkutan 16 mg/kg dozunda progesteron (P4) uygulandı. 3. gün 25 μl, 1 × 105 cfu/rat dozunda E. coli intrauterin olarak uygulandı. Çalışma sonunda tüm hayvanların yaşamına etik kurallara uygun bir şekilde son verildikten sonra kan örnekleri alındı. Alınan kan örneklerinden akyuvar (WBC) düzeyi için hemogram analizi yapıldı. Kan örneklerinden serum ayrıştırılarak ticari kit ile TNF-beta analizi yapılmıştır.

Bulgular: WBC (p=0.0004) ve TNF-beta (p=0.027) kontrol grubuna göre endometritis grubunda istatistiksel olarak daha yüksekti.

Sonuç: Sonuç olarak, endometritiste TNF-beta’nın artış göstermesi genital sistemdeki inflamasyonlar için tanı markırı olabilir. Fakat ilgili markır ile kapsamlı çalışmalar yapılarak etkinliği daha iyi ortaya konulabilir.

Keywords

• TNF-Beta
• WBC
• Endometritis
• Rat

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