A Molecularly Imprinted Polymer Based Biosensor for Electrochemical Impedance Spectroscopic Analysis

Year 2018, Volume: 18 Issue: 1, 39 - 44, 23.02.2018

Feride Şermin Utku Ozan Enver Özdemir Melahat Sevgül Bakay

Abstract

A molecularly imprinted
polymer (MIP)-based impedimetric biosensor was developed for the
electrochemical analysis of low-weight biological molecules. Synthetic
polymeric matrices with specific and selective recognition sites, which are
complementary to the shapes and sizes of the functional groups of analytes, can
be prepared using the molecular imprinting method. In this study, a small
molecule, tris(hydroxymethyl)aminomethane (TRIS), was used to coat a graphite
pencil tip with a TRIS-containing polyacrylamide gel to fabricate a working
electrode. The electrode modification and performance were evaluated using
cyclic voltammetry and electrochemical impedance spectroscopy. The
electrochemical properties of the modified electrodes were observed using an
electrochemical cell comprising a Ag/AgCl reference electrode, a Pt wire as the
counter electrode, and a pencil graphite tip as the working electrode using a
redox-phosphate buffer solution with different concentrations of TRIS and
Ethylenediaminetetraacetic acid (EDTA). The I–V and impedance performance of the chemically
modified graphite pencil-tip electrodes exhibited decreased conductance and
increased impedance correlating with  the
increase in TRIS concentration. Thus,
MIP-based small-molecule biosensor prototypes can be promising economical
replacements over other expensive sensors.

Keywords

Molecularly Imprinted Polymer, Cyclic Voltammetry, Electrochemical Impedance Spectroscopy, tris(hydroxymethyl)aminomethane, Pencil Graphite Electrode

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