Abstract
References
- Bellio P, Fagnani L, Nazzicone L, Celenza G (2021). New and simplified method for drug combination studies by checkerboard assay. MethodsX 8: 101543.
- Bravo HR, Copaja SV, Lazo W (1997). Antimicrobial activity of natural 2-benzoxazolinones and related derivatives. J Agric Food Chem 45: 3255-3257.
- Koksal M, Gokhan N, Erdogan H, Ozalp M, Ekizoglu M (2002). Synthesis of 3-(4-substituted benzoylmethyl)-2-benzoxazolinones and screening antimicrobial activities. Il Farmaco 57(7): 535-538.
- Ozalp M, Calis U, Gokhan N, Erdogan H (2001). Evaluation of antimicrobial activities of some 2-benzoxazolinone derivatives. FABAD J Pharm Sci 26: 169-172.
- Siugzdaite J, Lelesius R, Grybaite B, Vaickelioniene R, Mickevicius V (2024). Synthesis and biological studies of new 2-benzoxazolinone derivatives as antibacterial agents. Appl Sci 14: 4783.
- Verma H, Silakari O (2018). Chapter 10 - Benzoxazolinone: A scaffold with diverse pharmacological significance. Key Heterocycle Cores for Designing Multitargeting Molecules p. 343-367. Punjabi University, Patiala, India.
- Wikler MA (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard, vol. 26, p M7–A7. CLSI, Wayne, Pennsylvania.
In vitro Evaluation of Antibacterial Activity 2-benzoxazolinone and Its Combination Interaction with Ciprofloxacin
Abstract
In the present study, it was aimed to demonstrate the antibacterial and synergistic activities of 2-benzoxazolinone, a promising allelochemical, against Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 25923 via broth microdilution method. 2-benzoxazolinone exhibited promising antibacterial activity against all tested bacteria with 4 mg/mL against E. coli and E. faecalis, and K. pneumoniae and S. aureus 2 mg/mL against. When used in combination with ciprofloxacin, no antagonistic activity was observed. However, the combination interaction revealed indifferent activity against all tested strains. Overall, the antibacterial data obtained from the study demonstrated that 2-benzoxazolinone can further be used as a potential antibacterial agent in the treatment of bacterial infections.
References
- Bellio P, Fagnani L, Nazzicone L, Celenza G (2021). New and simplified method for drug combination studies by checkerboard assay. MethodsX 8: 101543.
- Bravo HR, Copaja SV, Lazo W (1997). Antimicrobial activity of natural 2-benzoxazolinones and related derivatives. J Agric Food Chem 45: 3255-3257.
- Koksal M, Gokhan N, Erdogan H, Ozalp M, Ekizoglu M (2002). Synthesis of 3-(4-substituted benzoylmethyl)-2-benzoxazolinones and screening antimicrobial activities. Il Farmaco 57(7): 535-538.
- Ozalp M, Calis U, Gokhan N, Erdogan H (2001). Evaluation of antimicrobial activities of some 2-benzoxazolinone derivatives. FABAD J Pharm Sci 26: 169-172.
- Siugzdaite J, Lelesius R, Grybaite B, Vaickelioniene R, Mickevicius V (2024). Synthesis and biological studies of new 2-benzoxazolinone derivatives as antibacterial agents. Appl Sci 14: 4783.
- Verma H, Silakari O (2018). Chapter 10 - Benzoxazolinone: A scaffold with diverse pharmacological significance. Key Heterocycle Cores for Designing Multitargeting Molecules p. 343-367. Punjabi University, Patiala, India.
- Wikler MA (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: Approved standard, vol. 26, p M7–A7. CLSI, Wayne, Pennsylvania.
Details
| Primary Language | English |
|---|---|
| Subjects | Pharmaceutical Microbiology |
| Journal Section | Research Article |
| Authors | |
| Publication Date | July 3, 2025 |
| Submission Date | April 30, 2025 |
| Acceptance Date | June 19, 2025 |
| Published in Issue | Year 2025 Volume: 8 Issue: 1 |
