An investigation of Helicobacter pyloriinfection in patients with inflammatory bowel disease using immunohistochemistry

Year 2012, Volume: 20 Issue: 3, 77 - 82, 01.12.2012

Özgür Bahadır Mesut Sezikli Fatih Güzelbulut Züleyha Akkan Çetinkaya Selvinaz Özkara

https://doi.org/10.17940/endoskopi.74821

Abstract

Background and Aims:Background and Aims: The aim of this study was to evaluate whether colonic mucosa is infected with Helicobacter pylori in patients with inşammatory bowel disease. Materials and Methods: The data of patients who underwent colonoscopy in the endoscopy unit were reviewed retrospectively. Of them, patients with or without inşammatory bowel disease who had at least three colon biopsy specimens including the terminal ileum, ascending colon, transverse colon, descending colon, sigmoid colon, or rectum were reviewed for the presence of gastric Helicobacter pyloriinfection. Patients with gastric Helicobacter pylori infection as evidenced by histopathologic analysis or C14urea breath test were included in the study. Patients with neoplasia or polyp and those with prior colonic resection were excluded from the study. Results: Totally, 60 patients with inşammatory bowel disease were included in this study. Sixty patients with normal colonoscopy results were included as the control group. None of the patients in either group had Helicobacter pylori infection in colonic biopsy specimens by immunohistochemical staining. Conclusions: Colonic Helicobacter pylori infection was not determined in either patients with inşammatory bowel disease or healthy controls using immunohistochemical staining. However, immunohistochemical staining might not be as sensitive as polymerase chain reaction for the detection of Helicobacter pylori in colonic mucosa. There is a need for studies to evaluate the presence of Helicobacter pylori infection in colonic

Keywords

Colonic mucosa, Helicobacter pylori, immunohistochemistry

İnflamatuvar bağırsak hastalığında kolonda Helikobakter pilori'nin immunohistokimyasal yöntemle araştırılması

Abstract

Amaç: Bu çalışmanın amacı inflamatuvar barsak hastalığı olan hastaların kolon mukozasının Helikobakter pilori ile enfekte olup olmadığını araştırmaktır. Gereç ve Yöntem: Endoskopi ünitesinde kolonoskopi yapılan hastaların kayıtları retrospektif olarak incelendi. İnflamatuvar barsak hastalığı olan ve olmayan hastalardan kolonoskopi sırasında terminal ileum, çekum, çıkan kolon, transvers kolon, inen kolon, sigmoid kolon veya rektumdan olmak üzere en az üç bölgeden biyopsi alınmış olanlar gastrik Helikobakter pilori enfeksiyonu varlığı açısından incelendi. Histopatolojik inceleme veya C14-üre nefes testi ile gastrik Helikobakter pilori enfeksiyonu saptananlar çalışmaya dahil edildi. Kolonoskopide neoplazi veya polip olanlar ve geçirilmiş kolon rezeksiyonu olanlar çalışmaya alınmadı. Bulgular: İnflamatuvar barsak hastalığı tanısı olan 60 hasta çalışmaya dahil edildi. Kontrol grubu da 60 hastadan oluştu. Her 2 grupta da hiçbir hastada immünhistokimyasal boyama ile kolonik Helikobakter pilori enfeksiyonu saptanmadı. Sonuç: İmmünhistokimyasal boyama ile kolonda Helikobakter pilori enfeksiyonu her 2 grupta da saptanamamıştır. İmmünhistokimyasal inceleme inflamatuar barsak hastalığı olan hastalarda polimeraz zincir reaksiyonu kadar sensitif olmayabilir. Kolonik Helikobakter pilori enfeksiyonu varlığını daha fazla sayıda hasta gruplarında ve polimeraz zincir reaksiyonu kullanarak araştıracak çalışmalara ihtiyaç vardır.

Keywords

İnflamatuvar barsak hastalığı, Helikobakter pilori

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