Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae: Spilomelinae)’ten izole edilen cry1, cry3 ve cry4 genlerini içeren yerel Bacillus thuringiensis (Berliner, 1915) (Bacteria: Bacillaceae) bakterisine ait insektisidal aktivite

Öz

Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae: Spilomelinae) şimşir üzerinde birincil zararlıdır. Bu zararlının larvaları yaprakları yiyerek zarar verir. Bitkinin fotosentez yapmasını engeller, sürgünlerin kabuğunu kemirir, kambiyum tabakasına zarar verir ve böylece şimşir sürgünlerini ve dallarını kuruturlar. Türkiye'de egzotik bir tür olan şimşir güvesi ilk olarak 2011 yılında İstanbul'da bahçe ve parklarda bulunmuş ve daha sonra; 2015 yılında Artvin ve Düzce'de, 2016 yılında Bartın'da yapılmıştır. Bu çalışmanın amacı, türün zararını göz önünde bulundurarak bu zararlı için bir kontrol yöntemi geliştirmektir. Bu kapsamda Mayıs 2021'de Artvin ilinden toplanan larvalardan bakteri izolasyonu yapılmış ve etkili bir izolat elde etmek için bakteri taraması yapılmıştır. Bu izolasyon sonunda Bacillus thuringiensis (Berliner, 1915) (Bacteria: Bacillaceae) bulunmuştur. Bu bakterinin cry gen analizi yapıldığında cry1, cry3 ve cry4 genlerini içerdiği belirlendi. Bu bakterinin böcek öldürücü aktivitesi C. perspectalis üzerinde test edilmiş ve %85'lik bir ölüm oranı elde edilmiştir.

Anahtar Kelimeler

• Bacillus thuringiensis
• biyolojik mücadele
• cry genleri
• Cydalima perspectalis
• mikrobiyoloji

Local isolate of Bacillus thuringiensis (Berliner, 1915) (Bacteria: Bacillaceae) from Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae: Spilomelinae) includes cry1, cry3 and cry4 genes and their insecticidal activities

Abstract

Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae: Spilomelinae) is a primary pest on boxwood. The larvae of this pest cause damage by eating the leaves. They prevent the plant from performing photosynthesis, gnaw the bark of the shoots, damage the cambium layer and thus dry the boxwood shoots and branches. The boxwood moth, which is an exotic species in Türkiye, was first found in gardens and parks in Istanbul in 2011 and subsequently in Artvin and Düzce in 2015, and Bartın in 2016. The aim of this study is to develop a control method for this pest by considering the damage of the species. In this context, bacteria were isolated from the larvae collected from Artvin in May 2021 and bacteria screening was conducted to obtain an effective isolate. At the end of this isolation, Bacillus thuringiensis (Berliner, 1915) (Bacteria: Bacillaceae) was found. When cry gene analysis of this bacterium was performed, it was determined that it contained cry1, cry3 and cry4 genes. The insecticidal activity of this bacterium was tested on C. perspectalis and a mortality rate of 85% was obtained.

Keywords

• Bacillus thuringiensis
• biological control
• cry genes
• Cydalima perspectalis
• microbiology

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