Entomopatojen nematod Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’nın Türk HBH hibrit ırkının bazı morfolojik özellikleri ve üreme kapasitesi üzerinde sıcaklığın ve depolama süresinin etkisi

Öz

Entomopatojen nematodlar (EPN), tarımsal zararlıların biyolojik mücadelesinde başarıyla kullanılmaktadır. Bu çalışmanın amacı farklı gün ve sıcaklıklarda depolanmış olan Infektif Juvenillerden (IJ) elde edilen hermafrodit bireylerin vücut uzunluğunun, yumurta çapının ve üreme gücünün belirlenmesidir. Bu çalışmada Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’nın Hibrit Irkı HBH kullanılmıştır. 15, 25 ve 35°C'de 7,14 ve 21 gün depolanmış olan IJ’ler 100 IJ dozunda Galleria mellonella L., 1758 (Lepidoptera: Pyralidae)’nın son dönem larvası üzerine inoküle edilmiştir. Enfeksiyonun gerçekleştiği 2. gün sonunda larvalar disekte edilmiş, hermafrodit bireyler ve yumurtalar elde edilmiştir. Üreme gücü ise enfeksiyon gerçekleştikten 10-12 gün sonra belirlenmiştir. Bu çalışma 2023 yılında Bursa Uludağ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarı’nda yürütülmüştür. Sonuç olarak, en uzun hermafrodit bireyler ve yumurta çapı 15°C’de 7 gün muhafaza edilmiş olan IJ’lerden elde edilmiştir. Bu değerler sırasıyla 6207.22 µm ve 55.65 µm olarak belirlenmiştir. En yüksek üreme gücüde aynı sıcaklık ve günde tutulmuş olan IJ’lerde görülmüştür. Bu değer 167.500 IJs/G. mellonella larva olarak belirlenmiştir. Bu çalışma farklı sıcaklık değerlerinin ve depolama süresinin EPN’lerin üzerindeki morfolojik etkilerin belirlenmesini içeren önemli bir çalışmadır.

Anahtar Kelimeler

• Vücut uzunluğu
• yumurta çapı
• hermafrodit
• Heterorhabditis bacteriophora
• üreme gücü

Efficiency of temperature and storage duration on some morphological measurements and reproductive capacity of the entomopathogenic nematode Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’s Turkish HBH hybrid strain

Abstract

Entomopathogenic nematodes (EPNs) are successfully used in the biological control of agricultural insect pests. This study aims to determine the body length of hermaphrodite individuals, egg diameter and reproductive capacity obtained from Infective Juveniles (IJs) stored at different temperatures and durations. Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae)’s Hybrid Strain HBH was used in the study. IJs stored at 15, 25 and 35°C for 7, 14 and 21 days were inoculated onto Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) last instar larvae at a dose of 100 IJs. On the 2nd day of infection, hermaphrodite individuals and eggs were obtained by dissecting the larvae. The reproductive capacity was determined 10-12 days after infection. The study was conducted in Bursa Uludağ University, Faculty of Agriculture, Plant Protection Department, Nematology Laboratory in 2023. In conclusion, the longest hermaphrodite individuals and egg diameter were obtained as 6207.22 µm and 55.65 µm, respectively from the IJs stored for 7 days at 15°C. The highest reproductive capacity was also observed as 167.500 IJs per G. mellonella larva in IJs stored under the same conditions with respect to temperature and time. This study is important for assessing the morphological effects of different temperature values and storage durations on EPNs.

Keywords

• Body length
• egg diameter
• hermaphrodite
• Heterorhabditis bacteriophora
• reproductive capacity

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