Identification of odorant receptor protein genes in two Cephid stem borers (Hymenoptera: Cephidae) by high-throughput sequencing

Year 2023, , 307 - 316, 09.10.2023

Mahir Budak

https://doi.org/10.16970/entoted.1337346

Abstract

Insects are well adapted organisms to the terrestrial life on Earth. The evolution of the odorant receptor family is one of the causes underpinning this remarkable adaptation. Odorant receptors (ORs) sense aromas in the environment and cause the insect to respond. The ability of phytophagous insects to detect odor signals from their hosts is crucial for mating, oviposition, and feeding. The family of odorant receptor genes in Cephidae, pest on some economically important plants, is little understood. Bioinformatic tools were used to analyze the genomic data of the two pest species, Syrista parreyssii (Spinola, 1843) (Hymenoptera: Cephidae) (a rose pest) and Pachycephus smyrnensis J.P.E.F. Stein, 1876 (Hymenoptera: Cephidae), (a poppy pest), to determine their odorant receptors. The whole genome sequencing of P. smyrnensis collected in Sivas in 2020 was performed by next generation sequencing and short reads of S. parreyssii genome were obtained from previous studies. Following bioinformatic analyses, 67 and 82 putative odorant receptor genes were identified and annotated for P. smyrnensis and S. parreyssii, respectively. The ORs of these two species were found to be organized as repetitive genes in five separate clusters. No species-specific OR genes were identified in any of the investigated species. As a result, it was hypothesized that host specificity was acquired through the combined effect of multiple ORs.

Keywords

Cephini, next-generation sequencing, odorant receptors, repetitive genes, Sawflies

Supporting Institution

Sivas Cumhuriyet University

Project Number

F-508

Thanks

This study is supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number F-508. The numerical calculations reported in this paper were partially performed at TÜBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources).

İki Cephid sap arısında (Hymenoptera: Cephidae) koku reseptör protein genlerinin yüksek verimli dizileme ile tanımlanması

Abstract

Böcekler, Dünya'daki karasal yaşama iyi uyum sağlamış organizmalardır. Koku reseptör ailesinin evrimi, bu olağanüstü adaptasyonun altında yatan nedenlerden biridir. Koku reseptörleri (OR'ler) çevredeki aromaları algılar ve böceğin tepki vermesine neden olur. Fitofag böceklerin konukçularından gelen koku sinyallerini algılama yeteneği çiftleşme, yumurtlama ve beslenme için çok önemlidir. Ekonomik açıdan önemli bazı bitkilerde zararlı olan Cephidae'deki koku reseptör genleri ailesi çok az anlaşılmıştır. Biyoinformatik araçlar, iki zararlı türün, Syrista parreyssi (Spinola, 1843) (Hymenoptera: Cephidae) (bir gül zararlısı) ve Pachycephus smyrnensis J.P.E.F. Stein, 1876 (Hymenoptera: Cephidae), (bir haşhaş zararlısı), koku reseptörlerini belirlemek amacıyla genomik verilerini analiz etmek için kullanılmıştır. Sivas'ta 2020 yılında toplanan P. smyrnensis tüm genom dizilemesi yeni nesil dizileme ile yapılmış ve S. parreyssii genomuna ait kısa okumalar ise önceki çalışmalardan elde edilmiştir. Analizler sonucunda P. smyrnensis'ten 67 olası koku reseptörü geni ve S. parreyssii'den 82 olası koku reseptörü geni tanımlandı ve açıklandı. Bu iki türün OR'lerinin beş ayrı kümede tekrarlayan genler olarak organize olduğu bulunmuştur. İncelenen türlerin hiçbirinde türe özgü OR genleri tespit edilmemiştir. Sonuç olarak, konakçı özgüllüğünün birden fazla OR'nin birleşik etkisi yoluyla kazanıldığı varsayılmıştır.

Keywords

Cephini, yeni nesil dizileme, koku almaçları, tekrarlayan genler, Testereli arılar

Project Number

F-508

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