Biber (Capsicum annuum L.) (Solanales: Solanaceae)’ de Me1 ve N genlerine bağlı moleküler markörlerin etkinliğinin karşılaştırılması

Öz

Biber (Capsicum annuum L.) (Solanales: Solanaceae) dünyada tüketilen en önemli tarımsal ürünlerinden biridir. Kök-ur nematodları (Meloidogyne spp.) biberde ciddi hasara neden olan başlıca zararlılardır. Ancak Kök-ur nematodlarının mücadelesinde bazı zorluklar vardır ve bulaşık bölgelerde en etkili mücadele yöntemlerden biri dayanıklı çeşitlerin kullanılmasıdır. Bu çalışmada Me1 ve N genlerine bağlı moleküler belirteçlerin etkinliği araştırılmıştır. Çalışma, 2022 yılında Akdeniz Üniversitesi Ziraat Fakültesi Bitki Koruma Bölümü Nematoloji laboratuvarında, laboratuvar ve kontrollü iklim odası koşullarında yürütülmüştür. İki ana çeşide (Charleston biberi ve Dolma biberi) ait biber genotipleri Meloidogyne incognita (Kofoid & White, 1919) Chitwood 1949 (Tylenchida: Heteroderidae) S6 izolatına karşı test edilmiş ve moleküler belirteçlerle taranmıştır. Sonuç olarak iki gene bağlı moleküler belirteçler patolojik testlerle uyumlu sonuçlar vermiştir. Bu belirteçler biber genotiplerinde markör destekli seleksiyonda başarılı bir şekilde kullanılabilir.

Anahtar Kelimeler

• Meloidogyne incognita
• patolojik test
• PCR primerler
• dayanıklılık

Comparison of effectiveness of molecular markers linked to Me1 and N genes in pepper (Capsicum annuum L.) (Solanales: Solanaceae)

Abstract

Pepper (Capsicum annuum L.) (Solanales: Solanaceae) is one of the most important agricultural products consumed in the world. Root-knot nematodes (RKNs (Meloidogyne spp.)) are major pests that occur dramatically damage on pepper. However, the management of RKNs has some difficulties and one of the most effective methods is using resistant cultivars in infested areas. In this study, the efficiency of molecular markers linked to Me1 and N genes was investigated. The study was conducted in laboratory and under controlled conditions at Akdeniz University Faculty of Agriculture, Department of Plant Protection Nematology Laboratory in 2022. Pepper genotypes belonging to two main varieties (Charleston pepper and Bell pepper) were tested against S6 isolate of Meloidogyne incognita (Kofoid & White, 1919) Chitwood 1949 (Tylenchida: Heteroderidae), and screened with molecular markers. As a result, molecular markers linked to two genes gave compatible results with pathologic tests. These markers can be successfully used for marker assisted selection in pepper genotypes.

Keywords

• Meloidogyne incognita
• pathologic tests
• PCR primers
• resistance

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