Bazı entomopatojen nematodların uzun süreli depolamaya fizyolojik tepkileri

Öz

Entomopatojen nematodlar (EPN) zararlıların mücadelesinde yaygınlıkla kullanılmaktadır. EPN’lerin özellikle depolama süresinin uzunluğunun belirlenmesi EPN’lerin etkili bir şekilde kullanılması açısından önemlidir. Bu çalışmanın amacı, farklı süreler boyunca depolanmış olan bazı EPN’lerin stok süreleri sonunda etkinlikleri ve üreme kapasitelerinin belirlenmesidir. Çalışmada Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH Hibrit Irkı, HBNL ve HB4 izolatı ve Steinernema feltiae Weiser, 1955 (Rhabditida: Steinernematidae) SADIÇ ve ST5 izolatları kullanılmıştır. 4ºC'de 6, 12, 18 ve 24 ay boyunca inkübe edilmiş olan infektif jüvenillerin (IJs) belirtilen süreler sonunda Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) son dönem larvaları üzerinde etkinlikleri ve üreme güçleri belirlenmiştir. Bu çalışma 2024 yılında Bursa Uludağ Üniversitesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarında gerçekleştirilmiştir. Sonuç olarak, G. mellonella larvaları üzerinde görülen en yüksek ölüm oranı 6 ay depolanmış olan H. bacteriophora HBH Hibrit Irkında %86.67 olarak belirlenmiştir. En yüksek üreme kapasitesi de aynı şekilde 6 ay depolanmış olan H. bacteriophora HBH Hibrit Irkında 153 000 IJs/ G. mellonella larva şeklinde belirlenmiştir. Bu çalışma, EPN'nin depolanma süresinin EPN etkinliği ve üreme kapasitesi üzerindeki etkilerinin belirlenmesi açısından önemli sonuçlar taşımaktadır.

Anahtar Kelimeler

• Heterorhabditis bacteriophora
• üreme kapasitesi
• Steinernema feltiae
• depolama süresi

Physiological reactions of some entomopathogenic nematodes to long-term storage

Abstract

Entomopathogenic nematodes (EPNs) are commonly used for pest control. Determining the optimal storage duration for EPNs is crucial for their effective utilization. The aim of this study is to determine the efficacy and reproductive capacities of some EPNs stored for different durations. Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH Hybrid Strain, HBNL, and HB4 isolates, as well as Steinernema feltiae Weiser, 1955 (Rhabditida: Steinernematidae) SADIÇ and ST5 isolates, were used in the study. The Infective Juveniles (IJs) stored at 4ºC for 6, 12, 18, and 24 months were assessed for their efficacy and reproductive capacities on last instar larvae of Galleria mellonella L., 1758 (Lepidoptera: Pyralidae) at the end of the periods. This study was conducted at Bursa Uludağ University, Plant Protection Department, Nematology Laboratory. The highest mortality rate observed on G. mellonella larvae was 86.67% on the H. bacteriophora HBH Hybrid Strain stored for 6 months. Similarly, the highest reproductive capacity was determined to be 153 000 IJs/G. mellonella larva, also on the H. bacteriophora HBH Hybrid Strain stored for 6 months. This study showed significant results in determining the effects of storage durations on the efficacy and reproductive capacity of the EPNs.

Keywords

• Heterorhabditis bacteriophora
• reproductive capacity
• Steinernema feltiae
• storage duration

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