Bazı Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae) izolatlarının Cryptolestes ferrugineus (Stephens, 1831) (Coleoptera: Laemophloeidae) üzerindeki ölüm oranı ve penetrasyon etkisinin değerlendirilmesi

Öz

Küçük kırma biti, Cryptolestes ferrugineus (Stephens, 1831) (Coleoptera: Laemophloeidae) depolanmış ürün ortamlarında ciddi zarara neden olan bir zararlıdır. Bu çalışmada, entomopatojen nematod Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae)’nin iki Türk izolatının ST6 ve TUR-S3'ün laboratuvar koşullarında ergin C. ferrugineus'a karşı insektisidal etkinliği değerlendirilmiştir. İnfektif juveniller (IJ'ler) beş konsantrasyon (50, 100, 150, 200 ve 250 IJs / Petri kabı) olarak uygulandı ve hem ölüm oranları hem de nematod penetrasyon seviyeleri belirlendi. Bu çalışma 2025 yılında Bursa Uludağ Üniversitesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarında gerçekleştirilmiştir. Sonuçlar, her iki izolat için de ölüm oranında doza bağlı net bir artış olduğunu, TUR-S3'ün 250 IJ'de en yüksek ölüm oranına (%93,33) ulaştığını, bunu ST6'nın (%86,67) izlediğini ortaya koymuştur. Penetrasyon oranları da konsantrasyonla önemli ölçüde artarak, penetrasyon ile ölüm oranı arasında güçlü bir pozitif korelasyon olduğunu desteklemektedir (ST6 için r=0.8113; TUR-S3 için r=0.8850). İkisi arasında TUR-S3 sürekli olarak üstün performans sergilemiş ve daha yüksek penetrasyon ve enfeksiyon etkinliği elde etmiştir. Bu Türk S. feltiae izolatlarının C. ferrugineus'a karşı patojenitesini gösteren ilk rapordur ve sürdürülebilir hasat sonrası zararlı yönetimi stratejilerine entegre edilmeleri için çevre dostu biyolojik kontrol ajanları olarak potansiyellerini vurgulamaktadır.

Anahtar Kelimeler

• Biyolojik mücadele
• entomopatojen nematod
• patojenite
• penetrasyon
• depolanmış ürün zararlısı

Evaluating the impact of some Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae) isolates on Cryptolestes ferrugineus (Stephens, 1831) (Coleoptera: Laemophloeidae) mortality and penetration

Abstract

The rusty grain beetle, Cryptolestes ferrugineus (Stephens, 1831) (Coleoptera: Laemophloeidae) is a destructive pest in stored-product environments. This study evaluated the insecticidal efficacy of two Turkish isolates of the entomopathogenic nematode Steinernema feltiae (Filipjev, 1934) (Rhabditida: Steinernematidae) ST6 and TUR-S3-against adult C. ferrugineus under laboratory conditions in 2025 at the Nematology Laboratory of the Department of Plant Protection, Bursa Uludağ University. Infective juveniles (IJs) were applied at five concentrations (50, 100, 150, 200 and 250 IJs per petri dish), and both mortality rates and nematode penetration levels were recorded. Results revealed a clear dose-dependent increase in mortality for both isolates, with TUR-S3 achieving the highest mortality (93.33%) at 250 IJs, followed by ST6 (86.67%). Penetration rates also increased significantly with concentration, supporting a strong positive correlation between penetration and mortality (r=0.8113 for ST6; r=0.8850 for TUR-S3). Among the two, TUR-S3 consistently exhibited superior performance, obtaining higher virulence and infection efficiency. This is the first report demonstrating the pathogenicity of Turkish S. feltiae isolates against C. ferrugineus, underscoring their potential as eco-friendly biocontrol agents for integration into sustainable post-harvest pest management strategies.

Keywords

• Biocontrol
• entomopathogenic nematode
• pathogenicity
• penetration
• stored-product pest

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