Toprakaltı termiti Heterotermes indicola (Wasmann) (Isoptera: Rhinotermitidae)’ya karşı indoxacarb ve chlorfenapyr’in laboratuvar koşullarında etkisi

Öz

Indoxacarb ve chlorfenapyrin’in, Toprakaltı termiti, Heterotermes indicola (Wasmann)’da beslenme engelleyici ve bireyler arasında taşınma etkileri 2013-2014 yıllarında Gıda ve Tarım Nükleer Enstitüsü (NIFA) (Peshawar, Pakistan)’nde laboratuvar testleri ile değerlendirilmiştir. Test edilen kimyasal konsantrasyonları indoxacarb için 1-100 ppm (ağırlık/ağırlık) ve chlorfenapyrin 1 ile 7 ppm (ağırlık/ağırlık) arasında değişmiştir. Gözlemler indoxacarb’ın 50 ppm üzerindeki dozlarda hızlı ölüme sebep olduğunu ortaya koymuştur. 10 ppm’de, ölüm nispeten daha yavaş gerçekleşmiş olup, ELT50 ve ELT90 (%50 ve %90 öldürücü etkili maruz kalma süresi) sırasıyla 6.7 ve 25.3 gün olarak kaydedilmiştir. 10 ppm altındaki konsantrasyonlarda, indoxacarb için %100 ölüm daha uzun sürede gerçekleşmiştir. Buna karşılık chlorfenapyrin, 1 ppm’lik en düşük konsantrasyon hariç, test edilen tüm konsantrasyonlarda hızlı bir ölüm oranına sebep olarak %100 ölüm oranı 9 günde saptanmış, ELT50 ve ELT90 ise sırasıyla 2,7 ve 8,6 gün şekilde hesaplanmıştır. Her iki termit öldürücünün 1 ila 100 ppm (ağırlık/ağırlık) aralığındaki çeşitli konsantrasyonları, beslenme engelleyici ve bireyler arasında taşınma çalışmaları için denenmiştir. Sonuçlar, indoxacarb’ın hiçbir konsantrasyonun, H. indicola’nın beslenmesini engellemediğini göstermiş ve sadece 100 ppm indoxacarb uygulanmış filtre kağıdının tüketimi %100 ölümle sonuçlanmıştır. Chlorfenapyrin 100 ppm altındaki konsantrasyonlarda beslenme engelleyici özellik göstermemiştir. Ölüm, konsantrasyon ne olursa olsun düşük kalmış ve beslenme engelleme testlerinde %60’ı aşmamıştır. Bireyler arasında taşınma çalışmalarda, indoxacarb taşıyıcılardan alıcılara 70 ve 100 ppm konsantrasyonlarda başarıyla taşınmıştır. Chlorfenapyrin taşınması genellikle düşük alıcı ölümüne sebep olmuş ve taşıyıcılardan alıcılara aktarımı, sadece 1 ppm için %80’in üzerinde olmuştur.

Anahtar Kelimeler

• Chlorfenapyr,caydırıcılık,Heterotermes indicola,indoxacarb,toksisite,transfer

Efficacy of indoxacarb and chlorfenapyr against Subterranean termite Heterotermes indicola (Wasmann) (Isoptera: Rhinotermitidae) in the laboratory

Abstract

Efficacy, feeding deterrence and transfer of indoxacarb and chlorfenapyr by the subterranean termite, Heterotermes indicola (Wasmann), were evaluated in laboratory tests at Nuclear Institute for Food and Agriculture (NIFA), Peshawar, Pakistan during 2013-2014. Chemical concentrations tested ranged from 1 to 100 ppm (wt/wt) of indoxacarb and 1 to 7 ppm (wt/wt) of chlorfenapyr. Observations revealed that indoxacarb caused rapid mortality at doses > 50 ppm. At 10 ppm, mortality was relatively slower and ELT50 and ELT90 (effective lethal exposure times for 50 and 90% mortality) were recorded as 6.7 and 25.3 d, respectively. At concentrations below 10 ppm, it took longer for indoxacarb to cause 100% mortality. In comparison, chlorfenapyr caused rapid mortality at all tested concentrations except the lowest concentration of 1 ppm, and 100% mortality occurred at 9 d, ELT50 and ELT90 calculated as 2.7 and 8.6 d, respectively. Various concentrations of both termiticides ranging from 1 to 100 ppm (wt/wt) were evaluated in feeding deterrence and transfer studies. The results showed that indoxacarb did not deter feeding of H. indicola at any concentration, and only consumption of filter paper treated with 100 ppm indoxacarb resulted in 100% mortality. Chlorfenapyr did not deter feeding at concentrations below 100 ppm. Mortality remained low regardless of concentration and did not exceed 60% in the feeding deterrence tests. In transfer studies, indoxacarb was successfully transferred from donors to recipients at concentrations of 70 and 100 ppm. Chlorfenapyr transfer generally caused low recipient mortality and transfer from donors to recipients was only evident at 1 ppm where recipient mortality exceeded 80%

Keywords

• Chlorfenapyr,deterrence,Heterotermes indicola,indoxacarb,toxicity,transfer

References

1. Badshah, H., Z. Salihah, A. Saljoqi & M. Shakur, 2004. Toxic effects of AK (Calotropis procera) plant extracts against termites (Heterotermes indicola and Coptotermes heimi) Isoptera: Rhinotermitidae. Pakistan Journal of Biological Sciences, 7: 1603-1606.
2. Baker, P. B. & D. E. Bellamy, 2006. Field and laboratory evaluation of persistence and bioavailability of soil termiticides to desert subterranean termite Heterotermes aureus (Isoptera: Rhinotermitidae). Journal of Economic Entomology, 99: 1345-1353.
3. Baker, P. B. & Y. Carriere, 2011. Effectiveness of commercial and experimental termite monitors for the desert subterranean termite Heterotermes aureus (Isoptera: Rhinotermitidae) in Southern Arizona. Journal of Economic Entomology, 104: 1339-1342.
4. Balachander, M., O. Remadevi & T. Sasidharan, 2013. Dissemination of Metarhizium anisopliae infection among the population of Odontotermes obesus (Isoptera: Termitidae) by augmenting the fungal conidia with attractants. Journal of Asia-Pacific Entomology, 16: 199-208.
5. Batista-Pereira, L. G., M. G. dos Santos, A. G. Correa, J. B. Fernandes, C. Dietrich, D. A. Pereira, O. C. Bueno & A. M. Costa-Leonardo, 2004. Electroantennographic responses of Heterotermes tenuis (Isoptera: Rhinotermitidae) to synthetic (3Z,6Z,8E)-dodecatrien-1-ol. Journal of the Brazilian Chemical Society, 15: 372-377.
6. Buczkowski, G., C. W. Scherer & G. W. Bennett, 2012. Toxicity and horizontal transfer of chlorantraniliprole in the eastern subterranean termite. Journal of Economic Entomology, 105: 1736-1745.
7. Farid, A., M. Misbah-ul-Haq, M. Ullah & A. Sattar, 2014. Potential of fipronil as a feeding toxicant against the subterranean termite Heterotermes indicola (Rhinotermitidae: Isoptera). Philippine Agricultural Scientist, 97: 73-78.
8. Gahlhoff, J. E. & P. G. Koehler, 2001. Penetration of the eastern subterranean termite into soil treated at various thicknesses and concentrations of Dursban TC and premise 75. Journal of Economic Entomology, 94: 486-491.

9. Gera, N. & S. Kumar, 2011. An addition to the Thar desert termites (Insecta: Isoptera). Indian Forester, 137: 387-390.
10. Hoi, B, 2007. Tunneling Behaviour and Barrier Penetration of Subterranean Termites with Special Reference to Coptotermes gestroi (Wasmann). M.S Thesis, University Sains, Malayasia.
11. Hu, X. P., 2005. Evaluation of efficacy and nonrepellency of indoxacarb and fipronil-treated soil at various concentrations and thicknesses against two subterranean termites (Isoptera: Rhinotermitidae). Journal of Economic Entomology, 98: 509-517.
12. Hu, X. P., D. Song & C. W. Scherer, 2005. Transfer of indoxacarb among workers of Coptotermes formosanus (Isoptera: Rhinotermitidae): effects of dose, donor: recipient ratio and post-exposure time. Pest Management Science, 61: 1209-1214.
13. Hu, X. P., D. Song & W. Presley, 2006. “Horizontal and vertical transfer of fipronil within functional colonies, pp.39-44”. In Proceedings: National Conference on Urban Entomology (21-24 May 2006, Embassy Suites Hotel Raleigh-Durham, North Carolina).
14. Iqbal, N. & S. Saeed, 2013. Toxicity of six new chemical insecticides against the termite, Microtermes mycophagus D. (Isoptera: Termitidae: Macrotermitinae). Pakistan Journal of Zoology, 45: 709-713.
15. Jenkins, T. M., 2006. “Termite population structure revealed by DNA marker technology, pp.139-143”. In Proceedings: National Conference on Urban Entomology (21-24 May 2006, Embassy Suites Hotel Raleigh-Durham, North Carolina).
16. Kubota, S., Y. Shono, N. Mito & K. Tsunoda, 2008. Lethal dose and horizontal transfer of bistrifluron, a benzoylphenylurea, in workers of the Formosan subterranean termite (Isoptera: Rhinotermitidae). Journal of Pesticide Science, 33 (3): 243-248.
17. Manzoor, F. & N. Mir, 2010. Survey of termite infested houses, indigenous building materials and construction techniques in Pakistan. Pakistan Journal of Zoology, 42: 693-696.
18. Manzoor, F., A. H. Sayyed, T. Rafique & S. A. Malik, 2012. Toxicity and repellency of different insecticides against Heterotermes indicola (Isoptera: Rhinotermitidae). Journal of Animal and Plant Sciences, 22: 65-71.
19. Mao, L. X., G. Henderson & C. W. Scherer, 2011. Toxicity of seven termiticides on the formosan and eastern subterranean termites. Journal of Economic Entomology, 104: 1002-1008.
20. Neoh, K. B., J. Hu, B. H. Yeoh & C. Y. Lee, 2012. Toxicity and horizontal transfer of chlorantraniliprole against the Asian subterranean termite Coptotermes gestroi (Wasmann): Effects of donor: recipient ratio, exposure duration and soil type. Pest Management Science, 68: 749-756.
21. Rust, M. K. & R. K. Saran, 2006. Toxicity, repellency, and transfer of Chlorfenapyr against western subterranean termites (Isoptera: Rhinotermitidae). Journal of Economic Entomology, 99: 864-872.
22. Salihah, Z., R. Khatoon, A. U. Khan & A. Sattar, 1993. “A termite trap, NIFA-TERMAP, for capturing large population of field population of Heterotermes indicola, pp. 395-400”. In Proceedings: 13th Pakistan Congress of Zoology (31 Mar – 01 Apr 1993, Islamabad, Pakistan).
23. Shelton, T. G. & J. K. Grace, 2003. Effects of exposure duration on transfer of nonrepellent termiticides among workers of Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). Journal of Economic Entomology, 96: 456-460.
24. Shelton, T. G., J. E. Mulrooney & T. L. Wagner, 2006. Transfer of chlorfenapyr among workers of Reticulitermes flavipes (Isoptera: Rhinotermitidae) in the laboratory. Journal of Economic Entomology, 99: 886-892.
25. Spomer, N. A., S. T. Kamble, M. A. Coffelt & C. W. Scherer, 2011. Movement of chlorantraniliprole and indoxacarb termiticides in sandy loam and silty clay loam soil columns used against subterranean termites (Isoptera: Rhinotermitidae). Sociobiology, 57: 201-213.
26. SPSS, 2007. SPSS for Windows, Version 16.0. Chicago, SPSS Inc.
27. Su, N. Y., 2003. Overview of the global distribution and control of the Formosan subterranean termite. Sociobiology, 41: 7-16.
28. Su, N. Y., 2005. Response of the formosan subterranean termites (Isoptera: Rhinotermitidae) to baits or nonrepellent termiticides in extended foraging arenas. Journal of Economic Entomology, 98: 2143-2152.
29. Su, N. Y. & R. H. Scheffrahn, 1988. Foraging population and territory of the formosan subterranean termite (Isoptera: Rhinotermitidae) in an urban environment. Sociobiology, 14: 353-359.
30. Su, N. Y. & R. H. Scheffrahn, 1993. Laboratory evaluation of two chitin synthesis inhibitors, hexaflumuron and diflubenzuron, as bait toxicants against formosan and astern subterranean termites (Isoptera: Rhinoterrnitidae). Journal of Economic Entomology, 86: 1453-1457.
31. Su, N. Y. & R. H. Scheffrahn, 2000. Termites as pest of buildings, pp. 437-453. In T. Abe, D. E. Bignell and M. Higashi (eds.), Termites: evolution, sociality, symbiosis, ecology. Kluwer Academic Publishers, Dordrecht, Boston, London.
32. Su, N. Y., M. Tamashiro & M. Haverty 1987. Characterization of slow-acting insecticides for the remedial control of the formosan subterranean termite (Isoptera: Rhinotermitidae). Journal of Economic Entomology, 80: 1-4.
33. Su, N. Y., E. M. Thoms, P. M. Ban & R. H. Scheffahn, 1995. Monitoring/baiting station to detect and eliminate foraging populations of subterranean termites (Isoptera: Rhinotermitidae) near structures. Journal of Economic Entomology, 88 (4): 932-936.
34. Thorne, B. L. & N. L. Breisch, 2001. Effects of sublethal exposure to imidacloprid on subsequent behavior of subterranean termite Reticulitermes virginicus (Isoptera: Rhinotermitidae). Journal of Economic Entomology, 94: 492-498.
35. Treacy, M., T. Miller, B. Black, I. Gard, D. Hunt & R. M. Hollingworth, 1994. Uncoupling activity and pesticidal properties of pyroles. Biochemical Society Transactions, 22: 244-247.
36. Wing, K. D., M. Sacher, Y. Kagaya, Y. Tsurubuchi, L. Mulderig, M. Connair & M. Schnee, 2000. Bioactivation and mode of action of the oxadiazine indoxacarb in insects. Crop Protection, 19: 537-545.
37. Yeoh, B. H. & C. Y. Lee, 2007. Tunneling responses of the Asian subterranean termite, Coptotermes gestroi in termiticide-treated sand (Isoptera: Rhinotermitidae). Sociobiology, 50: 457-468.