Toxicological and behavioral effects of some plant extract on Colorado potato beetle, Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae)

Abstract

Repellent, ovicidal and oviposition deterrent effects of six plant extracts (Heracleum platytaenium Boiss (Apiaceae), Humulus lupulus L. (Cannabaceae), Achillea millefolium L. (Asteraceae), Acanthus dioscoridis L. (Acanthaceae), Phlomoides tuberosa (L.) Moench (Lamiaceae), Bifora radians Bieb. (Apiaceae)) were tested on Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae) under laboratory conditions. Methanol extracts prepared from plant vegetative components were tested on L. decemlineata. Heracleum platytaenium extract was significantly more toxic against the egg stage than all other extracts, except for A. millefolium 5 d after treatment. It was followed by A. millefolium extract reducing the egg hatch rate to 15%. Significant mortality was not observed in the case of other plant extracts. In the second series of experiments, different dose-response bioassays with H. platytaenium against L. decemlineata eggs were conducted. The lowest egg hatch rate of 1% was observed at 7.5% [w/v (plant extract/acetone]. The greatest oviposition deterrent effect was seen with the H. platytaenium extract treatment, which resulted in no egg laying. Plant extracts showed a high level of repellent activity to L. decemlineata and their activity increased with extended incubation time. The greatest repellency was observed with the A. millefolium extract treatment, which gave 0.01% repellency in the first 15 min. These results show that H. platytaenium extract could be a useful toll in the control of L. decemlineata.

Keywords

• Biopesticide
• Colorado potato beetle
• ovicidal effect
• plant extract
• repellency

Bazı bitki ekstraktlarının Patates böceği [Leptinotarsa decemlineata Say,1824 (Coleoptera: Chrysomelidae)]’ne karşı toksikolojik ve davranışsal etkileri

Öz

Altı bitki ekstraktının [Heracleum platytaenium Boiss (Apiaceae), Humulus lupulus L. (Cannabaceae), Achillea millefolium L. (Asteraceae), Acanthus dioscoridis L. (Acanthaceae), Phlomoides tuberosa (L.) Moench (Lamiaceae), Bifora radians Bieb. (Apiaceae)], Leptinotarsa decemlineata Say 1824 (Coleoptera: Chrysomelidae)’ye karşı repellent, ovisidal ve yumurta bırakmayı engelleyici etkileri test edilmiştir. Bitkilerin vejetatif aksamlarından hazırlanan methanol ekstraktları L. decemlineata üzerinde test edilmiştir. En yüksek ovisidal etki beşinci gün itibari ile H. platytaenium uygulamasında gözlemlenmiş ve bunu A. millefolium ekstraktının etkisi izlemiştir. Heracleum platytaenium ekstraktının etkinliğini %15 yumurta açılım oranı ile A. millefolium ekstraktının etkinliği takip etmiştir. Diğer bitki ekstraktlarında önemli ölüm gözlenmemiştir. İkinci seri denemelerde Patates böceğinin yumurtalarına karşı H. platytaenium’un doz-etki çalışmaları yürütülmüştür. Önemli derecede düşük yumurta açılım oranı sadece %7.5 [w/v (bitki ekstraktı/aseton)] konsantrasyonda %1 etki ile gözlemlenmiştir. En yüksek yumurta bırakmayı engelleyici etki H. platytaenium ekstraktında görülmüş ve bu muamelede hiç yumurta bırakılmamıştır. Bitki ekstraktları yüksek oranda repellent aktivite göstermiş ve bu aktivite inkübasyon süresine bağlı olarak artmıştır. Test edilen bitki ekstraktları arasında en yüksek derecede uzaklaştırıcı etki %0.01’lik oransal değer ile ilk 15 dakikada A. millefolium ekstraktında saptanmıştır. Bu sonuçlar H. platytaenium ekstraktının L. decemlineata kontrolünde önemli bir potansiyele sahip olduğunu ortaya koymuştur.

Anahtar Kelimeler

• Biyopestisit
• Patates böceği
• yumurta bırakmayı engelleyici etki
• bitki ekstraktı
• repellent

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