Detection and identification of citrus long-horned beetle, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) a new pest in Antalya Province, Turkey by sequencing of mtCOI region

Abstract

In 2016, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) was recorded for the first time on Acer negundo L. in Antalya Province, Turkey. Genetic identification of adult samples was made based on approximate alignment of a 729-bp sequence of the mitochondrial cytochrome oxidase I gene region. The sequence alignment in the BLAST analysis from the NCBI Genbank showed 99% similarity with A. chinensis. The sequence data was deposited to NCBI Genbank. A phylogenetic analysis was performed by using the sequence data belonging to A. chinensis and some species of same genus obtained from the NCBI. According to the phylogenetic analysis, populations of A. chinensis from Antalya and China were placed in the same subgroup and therefore it is postulated that the origin of the Antalya population is China.   

Keywords

• Anoplophora chinensis
• DNA barcoding
• mtCOI
• phylogenetic analysis

Antalya ilinde yeni bir zararlı Turunçgil uzun antenli böceği, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae)’in tespiti ve mtCOI gen bölgesi sekansına göre tanımlanması

Öz

Bu çalışma ile Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) Antalya ilinde ilk kez 2016 yılında Acer negundo L. bitkisi üzerinde tespit edilmiştir. Bununla birlikte, çalışmada A. chinensis’e ait ergin bireylerden DNA ekstraksiyonu yapılmıştır. Elde edilen DNA örneklerinin mitokondrial sitokrom oksidaz I gen bölgesinin amplifikasyonu sonucunda yaklaşık 729 bç sekans dizilimi elde edilmiştir. Elde edilen veriler, NCBI Genbank’ da BLAST analizine tabi tutulmuş ve A. chinensis ile %99 benzerlik gösterdiği tespit edilmiştir. Sekans dizilimi NCBI’a kaydedilmiş, Genbank’ta A. chinensis ve aynı cinse ait bazı türlerin sekans verilerinden yararlanılarak filogenetik analiz yapılmıştır. Filogenetik analize göre Antalya A. chinensis popülasyonu Çin popülasyonu ile aynı alt grupta yer almış ve Antalya popülasyonunun kökeninin Çin olabileceği ağırlık kazanmıştır.

Anahtar Kelimeler

• Anoplophora chinensis
• DNA barkodlama
• mtCOI
• filogenetik analiz

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