Çukurova Bölgesi (Türkiye) pamuk alanlarında Aphis gossypii (Glover) (Hemiptera: Aphididae) neonikotinoid direnci

Abstract

Pamuk yaprakbiti, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), pamuk tarım alanlarında ana zararlılardandır. Neonicotinoidler, Akdeniz Bölgesi'nde A. gossypii ve diğer herbivor böceklerin mücadelesinde oldukça yaygın kullanılan önemli bir insektisit grubudur. Ancak, Çukurova Bölgesi'nde A. gossypii'ye karşı bazı insektisit uygulamaları yüksek oranlarda kullanılmasına rağmen başarısız olmuştur. Bu nedenle, bu bölgedeki pamuk alanlardan 2015-2016 toplanan A. gossypii popülasyonlarında imidacloprid ve thiamethoxam dayanıklılık düzeyi belirlemek amacıyla biyoassay ve enzim analizleri yapılmıştır. Analizler sonucunda imidacloprid için 54.6-206.5 (dirençlilik faktörü: RF) arasında, thiamethoxam için 5.7-65.7 arasında LD50 dayanıklılık katsayıları bulunmuştur. Kürkçüler (RF 206.55) popülasyonu imidacloprid için, Körkuyu (RF 65.72) popülasyonu da thiamethoxam için en yüksek LD50 değerine sahiptir. Enzim analizi istatistiki anlamda yüksek metabolik direnci ortaya çıkarmıştır. Her iki insektisit içinde en yüksek enzim akitviteleri, karboksil esteraz enzimi Körkuyu popülasyonunda 17,8 nM/dk/mg protein, glutatyon S-transferaz (GSTs) enzimi Bahçe popülasyonunda 142,3 nM/dk/mg protein ve Körkuyu popülasyonunda 3,8 nM/dk/mg protein ile en yüksek monooksigenaz P450 enzim aktivitesi bulunmuştur. Bu çalışma, Türkiye’de neonicotinoidlere karşı A. gosyypii’de direnç gelişmesini ve bu direncin kırılması için yeni yönetim stratejilerine ihtiyaç olduğunu ortaya koymuştur.

Keywords

• Aphis gossypii
• biyoassay
• pamuk
• neonicotinoid
• dirençlilik

Neonicotinoid resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields of Çukurova Region, Turkey

Abstract

Cotton aphid, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), is a major pest in cotton fields. Neonicotinoids are important and highly prevalent insecticides currently used against A. gossypii and other herbivorous insect pests in the Mediterranean Region of Turkey. However, some insecticide applications against A. gossypii in the Çukurova Region have failed despite using high rates. Therefore, bioassays and enzyme analyses were conducted to determine resistance to imidacloprid and thiamethoxam in A. gossypii populations collected in 2015-2016 from cotton fields in this region. Resistance factors (RF) were 54.6 to 206.5 fold for imidacloprid and 5.7 to 65.7 fold for thiamethoxam. Populations from Kürkçüler (RF 206.5) had the highest LD50 for imidacloprid and from Körkuyu (RF 65.7) for thiamethoxam. Enzyme analysis revealed statistically higher metabolic resistance. Maximum enzyme activities were 17.8, 142.3 and 3.8 nM/min/mg protein for carboxylesterase for in Körkuyu, for glutathione S-transferase in Bahçe and for cytochrome P450 monooxygenase in Körkuyu, respectively. This study revealed the development of resistance in A.gossypii to neonicotinoid insecticides in Turkey and the need for new management strategies to break this resistance.

Keywords

• Aphis gossypii
• biyoassay
• cotton
• neonicotinoid
• resistance

References

1. Ahmad, M., Arif, M. I. & Denholm, I., 2003. High resistance of field populations of the cotton aphid Aphis gossypii Glover (Homoptera:Aphididae) to pyrethroid insecticides in Pakistan. Journal of Economic Entomology, 96:875-878.
2. A. Elbert, M. Haas, B. Springer, W. Thielert, R. Nauen,2008. Applied aspects of neonicotinoid uses in crop protection, Pest Manag. Sci. 64:1099–1105. Avidov, Z. and I. Harpaz. 1969. Plant Pests of Israel. Israel Universities Press, Jerusalem, Israel. 600 pp. S18.
3. Bradford, M. M., 1976. A Rapid and Sensitiv Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein–Dye Binding. Anal. Biochem., 72: 248-254.
4. Chen, X., Li, F., Chen, A., Ma, K., Liang, P., Liu, Y., Song, D., Gao, X., 2017. Both point mutations and low expression levels of the nicotinic acetylcholine receptor β1 subunit are associated with imidacloprid resistance in an Aphis gossypii (Glover). Pesticide Biochemistry and Physiology Volume 141, Pages 1-8.
5. Deguine, J. P., 1996. The evolution of insecticide resistance in Aphis gossypii Glover (Hemiptera: Aphididae) in Cameroon, Resistant Pest Management News Letter 8 (1): 13-14.
6. Delorme, R., Auge, D., Bethenod, M.T., Villatte, F., 1997. Insecticide Resistance in a Strain of Aphis gossypii from Southern France. Pestic. Sci. 49:90-96.
7. Devonshire, A. L. And Sawicki. R. M., 1979. Insecticide -resistant Myzus persicae as an example of evolution by gene duplication. Nature 280: 140-141.
8. Devonshire, A. L. and Moores, G. D., 1982. A carboxylesterase with broad substrate specifity causes organophosphorus, carbamate and pyrethroid resistance in peach-potato aphids (Myzus persicae), Pestic. Biochem. Physiol., 18: 235-246.

9. Devonshire, A. L., 1989. Resistance of Aphids to Insecticides. World Crop Pests: Aphids, Their Biology, Natural Enemies and Control. W. Helle. Amsterdam, The Netherlands, Elsevier. Vol. C: 123-139.
10. Feyereisen, R., 2005. Insect Cytochrome P450. Comprehensive Molecular Insect Science: Biochemistry and Molecular Biology. Gilbert L.I., Iatrou K. and G. S.S. Amsterdam, Elsevier. 4: 1-77.
11. Furk, C. and Hines, C. M., 1993. Aspects of insecticide resistance in the melon and cotton aphid, Aphis gossypii (Hemiptera: Aphididae), Ann. appl. Biol., 123: 9-17.
12. Herron, G.A., Wilson, L.J., 2011. Neonicotinoid resistance in Aphis gossypii Glover (Aphididae: Hemiptera) from Australian cotton, Aust. J. Entomol. 50:93–98.
13. Han, Z., Moores, G.D., Denholm, I., Devonshire, A,L, 1998. Association between Biochemical Markers and Insecticide Resistance in the Cotton Aphid, Aphis gossypii Glover. Pestıcıde Bıochemıstry and Physıology 62:164–171.
14. Hansen, L. and Hodgson, G. E.,1971. Biochemical Characteristics of Insect Microsomes N-and O-demethylation, Biochemical Pharmacology, 20: 1569–1573.
15. Hemingway, J. and Georghiou, G. P.,1984. Differential suppression of organophosphorus resistance in Culex quinquefasciatus by the synergists IBP, DEF, and TPP. Pesticide Biochemistry and Physiology 21(1): 1-9.
16. Herron, G.A., Wilson, L.J., 2011. Neonicotinoid resistance in Aphis gossypii Glover (Aphididae: Hemiptera) from Australian cotton. Australian Journal of Entomology, 50:93–98.
17. IRAC, 2015. Cotton Aphid (Aphis gossypii). http://www.irac-online.org/documents/aphis-gossypii-irm-poster/?ext=pdf.
18. Gore, J., Cook, D., Catchot, A., Leonard, B.R., Stewart, S.D., Lorenz, G., 2013. Cotton aphid (Heteroptera: Aphididae) susceptibility to commercial and experimental insecticides in the Southern United States, J. Econ. Entomol, 106:1430–1439.
19. Karunker, I., Benting, J., Lueke, B.,Ponge, T., Nauen, R., Roditakis, E., Vontas, J., Gorman, K., Denholm, I., and Morin, S., 2008. Over-expression of cytochrome P450 CYP6CM1 is associated with high resistance to imidacloprid in the B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochemistry and Molecular Biology, 38(6): 634-644.
20. Kim,S., Kim, I., & Lee, M.,1986. Aphid species and their seasonal fluctuationsin vegetable crops. Korean Journal of Plant Protection, 25:129–131. Li, X., Schuler, M.A., and Berenbaum, M.R., 2007. Molecular mechanisms of metabolic resistance to synthetic and natural xenobiotics. Annual Review of Entomology 52(1): 231-253.
21. Marshall, K. L., Moran, C., Chen, Y., and Herron, G.A., 2012. Detection of kdr pyrethroid resistance in the cotton aphid, Aphis gossypii (Hemiptera: Aphididae), using a PCR-RFLP assay. Journal of Pesticide Science 37(2): 169-172.
22. Marshall, K. L., Herron, G.A. & Chen, Y., 2014. Neonicotinoid Resistance in Cotton Aphid from Australia. In: 17th Australian Cotton Conference. Cotton Research and Development Corporation, Broadbeach, Australia.
23. Moores, G. D., Han, Z. J., Denholm, I., and Devonshire, A.L., 1996. Two forms of insecticide insensitive acetylcholinesterase in Aphis gossypii, Brighton Crop Protection Conference-Pests and Diseases, 745-750.
24. Nauen, R., & Denholm, I., 2005. Resistance of insects to neonicotinoid insecticides: current status and future prospects. Archives of Insect Biochemistry and Physiology 58:200–215.
25. Nauen, R., Jeschke, P., and Copping, L., 2008. In Focus: Neonicotinoid insecticides Editorial. Pest Management Science 64(11): 1081-1081.
26. O’Brien, P. J., Abdel-Aal, Y. A., Ottea, J. A., and Graves, J. B., 1992. Relationship of insecticide resistance to carboxylesterases in Aphis gossypii (Homoptera: Aphididae) from midsouth cotton. Journal of Economic Entomology, 85(3): 651-657.
27. Jeschke, P., Nauen, R., Schindler, M., Elbert, A., 2011. Overview of the status and global strategy for neonicotinoids, J.Agric. Food Chem. 59:2897–2908.
28. Pears, L., M. and Davıdson, R.H., 1956. Insect ests of Farm, Garden, and Orchard. «Fifth Edition» John Wiley and Sons, Inc. New York. Chapman and Hail, Limited London.
29. Nauen, R., Jeschke, P., Copping, L., 2008. In focus:neonicotinoid insecticides,PestManag.Sci.64:1081.
30. Rauch, N. And Nauen, R., 2003. Identification of biochemical markers linked to neonicotinoid cross resistance in Bemisia tabaci (Hemiptera: Aleyrodidae). Archives of Insect Biochemistry and Physiology 54(4): 165-176.
31. Scott, J. G. and Wen, Z., 2001. Cytochromes P450 of insects: the tip of the iceberg. Pest Management Science, 57(10): 958-967.
32. Seyedebrahimi, S.S., Jahromi, K.T., Imani, S., Naveh, V.S., Hesami, S., 2015. Characterization of imidaclropid resistance in Aphis gossypii (Glover)(Hemiptera:Aphididae) in southern Iran. Turk.entomol.derg.,39(4):413-423.
33. Shang, Q., Pan, Y., Fang, K.,Xi, J., Brennan, J.A., 2012. Biochemical characterization of acetylcholinesterase, cytochrome P450 and cross-resistance in an omethoate-resistant strain of Aphis gossypii Glover. Crop Protection, 31:15-20.
34. Stumpf, N. & Nauen, R., 2002. Mechanistic studies on neonicotinoid crossresistance in Q-type Bemisia tabaci (Hemiptera: Aleyrodidae). Proceedings of the 10th IUPAC International Congress on the Chemistry of Crop Protection.
35. Shi, X. B., Shi, X. G., Wang, H.Y., Xia, X. M., and Wang, K. Y., 2011. Crossresistance of the imidacloprid-resistant population of Aphis gossypii Glover (Homoptera: Aphididae) to other neonicotinoid insecticides and changes in activities of related enzymes. Acta Entomologica Sinica 54(9): 1027-1033.
36. Saito, T. and Hama, H., 2000. Carboxylesterase isozymes responsible for organophosphate resistance in the cotton aphid, Aphis gossypii Glover (Homoptera: Aphididae), Appl. Entomol. Zool., 35 (1): 171-175.
37. Suzuki, K., Hama, H., Konno, Y., 1993. Carboxylesterase of the Cotton Aphid, Aphis gossypii Glover (Homoptera: Aphididae), Responsible for Fenitrothion Resistence as a Sequestering Protein. Appl. Entomol. Zool., 28 (4) : 439-450.
38. Saito, T. and Hama, H., 2000. Carboxylesterase isozymes responsible for organophosphate resistance in the cotton aphid, Aphis gossypii Glover (Homoptera: Aphididae), Appl. Entomol. Zool., 35 (1): 171-175.
39. Takada, H. and Murakami, Y., 1988. Esterase variation and insecticide resistance in Japanese Aphis gossypii, Entomol. Exp. Appl., 48: 37-41.
40. Tomlin, C. D. S. (ed.) 1997. The Pesticide Manual. 11th edition, The British Crop Protection Council, BCPC Publications, 1606 p., UK.
41. USDA, 2017. Foreign agricultural service, global agiricultural information network, gain raport number tr 7010.
42. Velioğlu, A.S., C Erdoğan, M.O. ,Gurkan, G.D., Moores, 2008. Determination of the Insecticide Resistance Mechanisms Using Biochemical Methods in the Aphis gossypii Glover (Hemiptera: Aphididae) Populations Damaging on Cotton in Turkey. Tarım Bilimleri Dergisi-Journal of Agricultural Sciences,Volume: 14, Issue: 2, p:116-123
43. Wang, K. I., Guo, Q. L., Xia, X. M., Wang, H. Y., and Liu, T. X., 2007. Resistance of Aphis gossypii (Homoptera: Aphididae) to selected insecticides on cotton from five cotton production regions in Shandong, China. Journal of Pesticide Science 32(4): 372-378.
44. Wang, Z., Yao, M., and Wu, Y., 2009. Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci Pest Manag Sci., www.soci.org.
45. Wang, K. I., Guo, Q. L., Xia, X. M., Wang, H. Y., and Liu, T. X., 2007. Resistance of Aphis gossypii (Homoptera: Aphididae) to selected insecticides on cotton from five cotton production regions in Shandong, China. Journal of Pesticide Science 32(4): 372-378.
46. Williams, D. E. and Reisfeld, R. A., 1964. Disc electrophoresis in polyacrylamide gels: extension to new conditions of pH and buffer, Ann. NY. Acad. Sci., 121: 373-38.