Samsun’da Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae)'dan yeni bir bakülovirüs izolatının ve farklı konukçularda patojenitesinin belirlenmesi

Öz

Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae), Avrupa, Asya ve Kuzey Afrika'ya dağılmış dünya çapında bir zararlıdır. Elma, armut, erik, söğüt ve meşede ekonomik kayıplara neden olur. Bu çalışmada, Türkiye'nin Orta Karadeniz Bölgesi’nde bulunan Samsun'dan 2015-2016 yıllarında toplanan M. neustria larvalarından bakulovirüs izole edilmiştir. Ölü larvalardan elde edilen oklüzyon badilerin (OB) elektron mikroskobu analizi, çoklu nükleokapsidlere sahip yeni bir izolat (ManeNPV-T4) olduğunu gösterdi. Kimura iki-parametre analizi ve filogenetik ağaç, ManeNPV-T4 izolatından elde edilen kısmi lef-8, lef-9 ve polh genlerinin birleştirilmiş nükleotit ve amino asit dizilerine dayanarak, diğer 51 baculovirüsünki ile karşılaştırıldı. Üçüncü evre M. neustria larvalarına uygulanan insektisidal aktivite testleri sonucunda %48 ile %100 arasında ölüm gözlendi. ManeNPV-T4'ün M. neustria için LC₅₀ değeri 0,78 x 10³ OB/ml olarak belirlendi. Ek olarak, viral izolat Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) ve Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) larvaları üzerinde %50’den düşük ölüm oranı oluşturdu. Sonuç olarak, bu yeni nükleopolihedrovirüs M. neustria ve diğer lepidopter larvalarına karşı da enfektiviteye sahiptir.

Anahtar Kelimeler

• Bakülovirüs,tespit,Malacosoma neustria,ölüm oranı

Characterization of a novel baculovirus isolate from Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) in Samsun and its pathogenicity in different hosts

Abstract

Malacosoma neustria (Linnaeus, 1758) (Lepidoptera: Lasiocampidae) causes economic losses in apple, pear, plum, willow, oak and other economically important trees. In this study, an alphabaculovirus was isolated from the larval population of M. neustria from Samsun in the central Black Sea Region of Turkey between 2015 and 2016. Electron microscope analysis of occlusion bodies (OBs) obtained from dead larvae showed that the nucleocapsids of a new isolate (ManeNPV-T4) are multiply enveloped. The Kimura two-parameter analysis and the phylogenetic tree were performed based on concatenated nucleotide and amino acid sequences of the partial lef-8, lef-9 and polh genes from ManeNPV-T4 isolate compared to those of other 51 baculoviruses. Insecticidal activity tests against third instar M. neustria larvae produced 48 to 100% mortalities. The LC₅₀ of ManeNPV-T4 was 0.78 x 10³ OBs/ml in M. neustria. Additionally, the isolate caused mortalities lower than 50% in Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Hyphantria cunea (Drury, 1773) (Lepidoptera: Arctiidae) and Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) larvae. Consequently, the new nucleopolyhedrovirus is infectious on M. neustria larvae and other lepidopterans.

Keywords

• Baculovirus,Malacosoma neustria,identification,mortality

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