Helochares Mulsant, 1844 ve Coelostoma Brullé, 1835 (Coleoptera: Hydrophilidae) cinslerinin yağ asiti kompozisyonuna taksonomik bir yaklaşım

Öz

Bu çalışmada, Bingöl İli’nden toplanan Hydrophilidae (Coleoptera) familyasının Helochares Mulsant, 1844 ve Coelostoma Brullé, 1835 cinsine (Coelostoma orbiculare Fabricius, 1775; Coelostoma transcaspicum Reitter, 1906; Helochares obscurus Müller, 1776; Helochares lividus Forster, 1771) ait böceklerin toplam lipit ve yağ asiti kompozisyonu belirlenmiştir. Örnekler 2015 yılında toplanmıştır. Böceklerin yağ asiti bileşeni gaz kromatografisi (GC-MS) ile belirlenmiştir. Toplam doymuş yağ asitleri (ΣSFA) %23.9-40.8, toplam tekli doymamış yağ asitleri (ΣMUFA) %21.6-53.2 ve toplam çoklu doymamış yağ asitleri (ΣPUFA) %14.3-27.4 arasında değişim göstermiştir. SFA’ dan miristik asit (14: 0), pentadekanoik asit (15: 0) palmitik asit (16: 0), heptadekanoik asit (17: 0); MUFA’dan palmitoleik asit (16: 1n-7), oleik asit (18: 1n-9); ve PUFA’ dan linoleik asit (18: 2n-6), linolenik asit (18: 3n-3), araşidonik asit (ARA, 20: 4n-6), eikosapentaenoik asit (EPA, 20: 5n-3) en önemli yağ asitleri olarak saptanmıştır. Hydrophilidae familyası bireylerinde sadece türler arasındaki farkın nispeten önemli olduğu (R=0.63), alt familyalar (R=0.17) ve cinsler (R=0.17) arasındaki farkın kısmen önemli olduğu ve familyalar (R=0.08) arasındaki farkın önemli olmadığı ANOSIM sonuçlarıyla ortaya konulmuştur.

Anahtar Kelimeler

• Bingöl
• kemotaksonomik yaklaşım
• Coelostoma
• yağ asitleri
• Helochares

A chemotaxonomic approach to fatty acid composition of the genera Helochares Mulsant, 1844 and Coelostoma Brullé, 1835 (Coleoptera: Hydrophilidae)

Abstract

In this study, total lipid and fatty acid composition of insects belonging to the genera Helochares Mulsant, 1844 and Coelostoma Brullé, 1835 (Coelostoma orbiculare Fabricius, 1775; Coelostoma transcaspicum Reitter, 1906; Helochares obscurus Müller, 1776; Helochares lividus Forster, 1771) of Hydrophilidae (Coleoptera) family collected from Bingöl Province were determined. Specimens were collected in 2015. Fatty acid component of the insects was determined by mass-gas chromatography (GC-MS). Total saturated fatty acids (ΣSFA) were between 23.9-40.8%, total monounsaturated fatty acids (ΣMUFA) were 21.6-53.2% and total polyunsaturated fatty acids (ΣPUFA) were 14.3-27.4%. Myristic acid (14:0), pentadecanoic acid (15:0) palmitic acid (16:0), heptadecanoic acid (17:0) from SFA, palmitoleic acid (16:1n-7), oleic acid (18:1n-9) from MUFA; linoleic acid (18:2n-6), linolenic acid (18:3n-3), arachidonic acid (ARA, 20: 4n-6), eicosapentaenoic acid (EPA, 20: 5n-3) were the most important fatty acids. ANOSIM results showed that only the difference among the species was significant (R=0.63); the difference among subfamilies (R=0.17) and among the genera (R=0.17) were partially significant and that the difference among the families (R=0.08) was not significant.

Keywords

• Bingöl
• chemotaxonomic approach
• Coelostoma
• fatty acids
• Helochares

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