Bazı Tabanidae (Diptera) türlerinin karyotip karakterizasyonu

Öz

Anahtar Kelimeler

• At sinekleri, Tabanidae, Diptera, Insecta, karyotip analizi

Karyotype characterization of some Tabanidae (Diptera) species

Abstract

Karyotypic features of Tabanidae (Diptera: Insecta) are largely unexplored. In this study, karyotypes of some horse fly species, Tabanus quatuornotatus Meigen, 1820, T. sudeticus Zeller, 1842, Dasyrhamphis umbrinus (Meigen, 1820), Atylotus loewianus (Villeneuve, 1920) and Haematopota italica Meigen, 1804 are described for the first time, and also, karyotypes of T. autumnalis Linnaeus, 1761, T. bromius (Linnaeus, 1761), T. bifarius Loew, 1858, T. unifasciatus (Loew, 1858), A. fulvus (Meigen, 1820) are confirmed. Karyotypic differences between six genera in Tabanidae will be discussed. Adult and larval specimens were collected, injected cholchicine and brought to the laboratory. After 24-48 hours tissues were dissected, fixed and dyed, respectively. After dying, preparations were made by squashing tissues and investigated with light microscope and photographed. Based on the results of the present study, the chromosome number in the following reads as follows; T. autumnalis 2n=14, T. bromius 2n=10, T. bifarius 2n=16, T. quatuornotatus 2n=16, T. sudeticus 2n=14, T. unifasciatus 2n=12, D. umbrinus 2n=10, A. fulvus 2n=18, A. loewianus 2n=18 and H. italica 2n=14 chromosome number. Chromosome number, chromosome arm length and arm ratio, location of centromere of these species is reported and evaluated

Keywords

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References

1. Barros, A. T. M., 2001. Seasonality and relative abundance of Tabanidae (Diptera) captured on horses in the Pantanal, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, 96 (7): 917-923.
2. Blackman, R. L., 1985. Chromosome numbers in the Aphid Amphorophora tuberculata (Homoptera, Apididae). Chromosoma, 92: 357-362.
3. Blackman, R. L., S. E. Halbert & T. W. Carroll, 1990. Association between karyotype and host plant in Corn leaf aphid (Homoptera, Aphidae) in the Northwestern United States. Environmental Entomololoy, 19 (3): 609-611.
4. Boyes, J. W. & A. Wilkes, 1972. Chromosomes of Tabanidae. Canadian Journal of Genetics and Cytology, 14 (1): 95–104.
5. Chubareva, L. A. & N. A. Petrova, 2006. B-Chromosome polymorphism of Blackflies (Diptera, Simulidae) in Northwestern Russia. Tsitologiya, 48 (3): 253-263.
6. Chvala, M., L. Lyneborg & J. Moucha, 1972. The Horse Flies of Europe (Diptera: Tabanidae), Entomological Society of Copenhauge, E. W. Classey Ltd. Hampton, pp. 1-502.
7. Chvala, M. 1988. Cataloque of Palaearctic Diptera, Family: Tabanidae, Ed. Soss, A. and Papp, L. Vol. 5: 97-171.
8. Chvala, M. & J. Jezek, 1997. Aquatic Insects of North Europe Taxonomic Handbook, Volume 2. Ed. Anders N. Nilsson, 440pp.

9. Coluzzi, M., A. Sabatini, A. Della Torre, M. A. Di Deco & V. Petrarca, 2002. Polytene chromosome analysis of the Anopheles gambiae Species. Complex Science, 298 (5597): 1415-1418.
10. Cook, L. G., P. J. Gullan & H. E. Trueman, 2002. A preliminary phylogeny of the scale insects (Hemiptera: Sternorrhyncha) based on nuclear small subunit ribosomal DNA. Molecular Phylogenetics and Evolution,25: 43-52.
11. Crosskey, R. W. & B. F. Crosskey, 1955. The Horse-Flies (Diptera: Tabanidae) of Nigeria and British Camerons. Transactions of the Entomological Society of London, 106 (8): 341-374.
12. De Prins, J., W. De Prins & U. Dall’asta, 2002a. The resent spreading of Cameraria ohridella (Lepidoptera: Gracillariidae) in Belgium. Bultein De L’Institut Royal Des Sciences Naturelles De Belgique, 72 : 165-170.
13. De Prins, J., W. De Prins & U. Dall’asta, 2002b. The karyotype of Cameraria ohridella (Lepidoptera: Gracillariidae). Vlaamse Vereniging Voor Entomologie, 30: 5-10.
14. Fedorova, M. V., R. D. Zhantiev & V. E. Gokhman, 1998. Karyotypes of Mole crickets (Orthoptera, Gryllotalpidae) of the European part of USSR and the Caucasus. Zoologicheskii Zhurnal,70 (7): 43-50.
15. Ferreira R. L. M., A. L. Henriques & J. A. Rafael, 2002. Activity of Tabanids (Insecta: Diptera: Tabanidae) Attacking the Reptiles Caiman crocodilus (L.) (Alligatoridae) and Eunectes murinus (L.) (Boidae), in the Central Amazon, Brazil, Memórias do Instituto Oswaldo Cruz, 97(1): 133-136.
16. Gokhman, V. E., 2004. Karyotype evolution in parasitic wasps (Hymenoptera), Zoologicheskii Zhurnal, 83 (8): 961-970.
17. Gokhman, V. E., 2006. Implication of chromosome analysis for taxonomy of parasitic wasps (Hymenoptera). Entomological Review, 86 (1): 1-10.
18. Gokhman, V. E. & V. G. Kuznetsova, 2006. Comparative insect karyology: current state and applications. Entomological Review, 86 (3): 352-368.
19. Hota, J. & S. C. Patnatik, 1989. A study of chromosomes and their C- band patterns in seven species of Acrididae (Orthoptera). Caryologia, 42: 81-89.
20. Ivanischuk, P. P., 1983. Karyotypical Differences of Some Horse-flies Subspecies (Tabanidae, Diptera) (in Russian), MSc Thesis, Ivanovo State Medical Academy Moscow, 85 pp.
21. Ivanischuk, P. P., 1986. Comparative Karyology, Morphology and Ecology of the USSR Fauna Horseflies (Diptera, Tabanidae), 03.00.09-Entomology PH. D. Thesis, Ivanovo State Medical Academy, Moscow 126 pp.
22. Leclercq, M., 1985. Recent Additions and Sinonym in Palearctic Tabanidae (Diptera), MYIA, 3: 341-345.
23. Levan, A. K., K. Fredga & A. Sandberg, 1964. Nomencrature for centromeric position on chromosomes. Hereditas, 52: 201-220.
24. Krinsky W. L., 1976. Animal disease agents transmited by horse flies (Diptera: Tabanidae). Journal of Medical Entomology, 13: 225- 275.
25. Maltempi, P. P. P. & R. M. P. Avancini, 2000. Chytogenetics of neotropical fresh fly Pattonella intermutans (Diptera: Sarcophagidae). Genetics and Molecular Biology, 23 (3): 563-567.
26. Maltempi, P. P. P. & R. M. P. Avancini, 2001. C-banding and FISH in chromosomes of the blow flies Chrysomya megacephala and Chrysomya putoria (Diptera: Calliphoridae). Memórias do Instituto Oswaldo Cruz, 96 (3): 371-377.
27. Rao P. N. & K. S. Rai, 1987. Comparative karyotypes and chromosomal evolution in some genera of Nematocerous (Diptera, Nematocera) families. Annals of the Entomological Society of America, 80: 321-332.
28. Rimma, A. V., 1993. The Larvae of Horsefly (Diptera, Tabanidae) of the Palearctic Fauna (ecology, Morphology, Systhematic, Genesis of the Fauna Aspects and Their Parasites). PH. D. Thesis, Kiev, 125pp.
29. Rodriguez, S. G., L. M. Mola, A. G. Papeschi & C. L. Scioscia, 2002. Chytogenetic heterogeneity in common haplogyne spiders from Argentina (Arachnida: Araneae). The Journal of Arachnology, 30: 47-56.
30. Squitier, J. M., 1998. Deer Flies, Yellow Flies and Horse Flies, Chrysops, Diachlorus, and Tabanus spp., Featured Creatures from the Entomology and Nematology Department. University of Florida. EENY-028.
31. Stahlavsky, F., J. Kral, M. S. Harvey & C. R. A. Haddad, 2006. Karyotype study on the Pseudoscorpion families Geogarypidae, Garypinidae and Olpiidae (Arachnida: Pseudoscorpiones). European Journal of Entomology, 103: 227-289.
32. Warchalowska-Silwa, E. & A. G. Bugrov, 1998. Karyotypes and cbanding patterns of some phaneropterinae katydids (Orthoptera: Tettigonioidae) with special attention to a post-reductional division of the Neo-X and Neo-Y sex chromosomes in Isophya hemiptera. Folia Biology (Krakow), 46 (1-2): 119-125.