Molecular diagnosis of Globodera rostochiensis Wollenweber (Tylenchida: Heteroderidae) in the potato growing areas of Aegean Region, Turkey

Abstract

DNA isolation was done by the 8 different cyst populations collected from the potato growing areas of Aegean Region (Turkey) between 2006-2008. As a result of Multiplex PCR conducted with species-specific primer, composing of band is observed in 435 bp as specific to Globodera rostochiensis Wollenweber (Tylenchida: Heteroderidae). By doing PCR with rDNA2 and rDNA1.58S primers of the same sample, as a consequence of cropping with Hinf l enzyme, in 233bp and 522bp band were confirmed as specific to G. rostochiensis. In this study, other potato cyst nematodes, Globodera pallida Stone, 1973 was not determined. Globodera rostochiensis was determined previously by using morphometric methods in Turkey. The identification of G. rostochiensis was confirmed by molecular methods with this study. 

Keywords

• Potato cyst nematode
• Globodera rostochiensis
• potato
• molecular diagnosis

Ege Bölgesi patates alanlarında Globodera rostochiensis Wollenweber, (Tylenchida: Heteroderidae)’in moleküler yöntemlerle saptanması

Öz

Ege Bölgesi’nde patates alanlarından 2006-2008 yıllarında 8 adet kist nematodu populasyonu toplanarak, elde edilen kistlerden DNA izolasyonu yapılmıştır. Türe özgü spesifik primerler ile gerçekleştirilen Multipleks PCR sonucunda Globodera rostochiensis Wollenweber, (Tylenchida: Heteroderidae)’e özgü 435 bp de bant oluşumu gözlenmiştir. Aynı örneklerin rDNA2 ve rDNA1.58S primerleri ile PCR yapılarak Hinf l enzimi kesimi sonucunda G. rostochiensis’e özgü 233bp ve 522bp.de bant verdiği tespit edilmiştir. Bu çalışmada patates kist nematodlarından Globodera pallida Stone, 1973 tespit edilmemiştir. Türkiye’de daha önce morfometrik yöntemlerle saptanan, G. rostochiensis’ in varlığı moleküler yöntemlerle de ilk defa belirlenmiştir

Anahtar Kelimeler

• Patates kist nematodu
• Globodera rostochiensis
• patates
• moleküler teşhis

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