Protein patterns in the salivary gland of the sunn pest, Eurygaster integriceps (Put.) (Hemiptera: Scutelleridae)

Abstract

Keywords

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Süne [Eurygaster integriceps (Put.) (Hemiptera: Scutelleridae)]'nin salya bezindeki protein desenleri

Öz

Hemipteradaki salya bezleri, ağız dışındaki sindirimde etkili olan, böcek-bitki ilişkileri açısından önemli proteinleri içerir. Bu çalışmada Süne’nin beşinci dönem nimfleri ve ergin bireylerinde salya bezlerinin protein dokularının karşılaştırılmasında protomiks tekniklerinden yararlanılmıştır. Belirlenen 139 leke şeklindeki desenlerden 31 tanesi, proteinlerin tanılanması için seçilmiştir. 13 protein lekesi iki yaşam dönemi arasında farklı olarak belirlenmiştir. Ergin bireylerde tripsin, arginin kinaz ve fofolipaz A2 artış gösterirken, beşinci dönem nimflerde ise kimotripsin, arjinin metiltransferaz ve endonukleaz salyası daha yüksek miktarda gözlenmiştir. Diğer 18 leke salya bezinin protein haritasını oluşturmak için seçilmiştir. Beşinci dönem nimfler ile ergin bireylerde; ssp3 serin proteazları, alfa-amilaz, glukoz dehidrojenaz, vakuolar H+ATPase ve trombostasin-3’ün aynı oranlarda olduğu belirlenmiştir. Proteinlerin tanılanması ve salya bezlerindeki fizyolojik rollerinin belirlenmesi Süne-buğday etkileşimlerini aydınlatmak için önemli bir adımdır

Anahtar Kelimeler

• Salya bezi, proteomikler, pis kokulu böcek, enzim, sindirim

References

1. Alborn, H. T., T. V. Hansen, T. H. Jones, D. C. Bennett, J. H. Tumlinson, E. A. Schmelz & E. A. Peter, 2007. Disulfooxy fatty acids from american bird grasshopper Schistocerca americana, elicitors of plant volatiles. Proceeding of the National Academy of Sciences of the USA, 104: 12976-12981.
2. Alborn, H. T., T. C. J. Tulings, T. H. Jones, G. Stenhagen, J. H. Loughri & J. H. Tumlison. 1997. An elicitor of plant volatiles from beet armyworm oral secretion. Science, 276: 945-949.
3. Applebaum, S. W. 1985. "Biochemistry of Digestion. pp. 279-307". In: Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol.4. (Eds: G. A. Kerkut and L. L. Gilbert). Pergamon Press, London, 743 pp.
4. Barbosa Pereira, P. J., V. Lozanov, A. Patthy, R. Huber, W. Bode, S. Pongor & S. Strobl, S. 1999. Specific inhibition of insect α-amylases: yellow meal worm α-amylase in complex with the Amaranth α- amylase inhibitor at 2.0 Å resolution. Structure, 7: 1079-1088.
5. Boyd, D. W. 2003. Digestive enzymes and stylet morphology of Deraecoris nigritulus (Uhler) (Hemiptera: Miridae) reflect adaptions for predatory habits. Annals of the Entomological Society of America, 96: 667- 671.
6. Calvo, E & C. M. Ribeiro, 2006. A novel secreted endonuclease from Culex quinquefasciatus salivary glands. The Journal of Experimental Biology, 209: 2651-2659.
7. Carlini, C. R. & M. F. Grossi-de-Sa, 2002. Plant toxic proteins with insecticidal properties: A review on their potentialities as bioinsecticides. Toxicon, 40: 1515-1539.
8. D’Amico, S., C.Gerday, & G. Feller, 2000. Structural similarities and evolutionary relationships in chloride-dependent α-amylases. Gene, 253: 95-105.

9. Eichenseer, H., M. C. Mathews, J. L. Bi, J. B. Murphy & G. W. Felton, 1999. Salivary glucose oxidase: multifunctional roles for Helicoverpa zea?. Archives Insect Biochemistry and Physiology, 42: 99-109.
10. Franco, O. L., F. R. RigdenMelo, & M. F. Grossi-de-Sa, 2002. Plant α-amylase inhibitors and their interaction with insect α-amylases, structure, function and potential for crop protection. European Journal of Biochemistry, 269: 397-412.
11. Habibi, J., T. A. Courdon, E. A. Backus, S. L. Brandt, R. M. Wagner, M. K. Wright & J. E. Huesing, 2008. Morphology and histology of the alimentary canal of Lygus hesperus (Heteroptera: Cimicomorpha: Miridae). Annals of the Entomiological Society of America, 101: 159-171.
12. Hamilton, J. V., R. J. L. Munks, S. M. Lehane, & M. J. Lehane, 2002. Association of midgut defensin with a novel serine protease in the blood- sucking fly Stomoxys calcitrans. Insect Molecular Biology, 11: 195-207.
13. Harmel, N., E. Letocart, A. Cherqui, P. Giordanengo, G. Mazzucchelii, F. Guillonneau, E. Depauw, E. Haubruge & F. Francis, 2008. Identification of aphid salivary proteins: a proteomic investigation of Myzus persicae. Insect Molecular Biology, 17: 165-174.
14. Iulek, J., O. L. Franco, M. Silva, C. T. Slivinski, C. Bloch Jr., D. J. Rigden & M. F. Grossi-de- Sa, 2000. Purification, biochemical characterization and partial primary structure of a new α-amylase inhibitor from secale cereal (rye). International Journal of Biochemistry and Cell Biology, 32: 1195-1204.
15. Javaheri, M., C. W. Scheafer & J. D. Lattin, 2009. "Shield bugs (Scutelleridae). pp 457-503". In: Heteroptera of economic Importance, (Eds: C. W. Scheafer & A. R. Panizi). CRC Press, Washington, USA, 856 pp.
16. Jouanian, L., M. Bonade-Bottino, C. Giard, G. Morrot & M. Giband, 1998. Transgenic plants for insect resistance. Plant Science, 131:1-11.
17. Oliveira-Neto, O. B., J. A. N. Batista, D. J. Rigden, O. L. Franco, R. Falcao, R. R. Fragoso, L. V. Mello, R. C. dos Santos & M. F. Grossi-de-Sa, 2003. Molecular cloning of α- amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance. Journal of Protein Chemistry, 22: 77-87.
18. Rodrigues, F. G., M. N.,Santos,T. X., de Carvalho, B. C., Rocha, M. A.,Riehle, P. F., Pimenta, E. G., Abraham, M., Jacobs-Lorena,C. F., Alves de Brito & L.A.Moreira, 2008. Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development. Insect Molecular Biology, 17: 175-183.
19. Strobl, S., K. Maskos, G. Wiegand, R. Huber, F. X. Gomis-Ruth, & R. Glockshuber, 1998. A novel strategy for inhibition of α-amylases: yellow meal worm α-amylase in complex with the ragi bifunctional inhibitor at 2.5 Å resolution. Structure, 6: 911-921.
20. Syntichaki, P., C. C. Samara, & N. Tavernarakis, 2005. The vacuolar H+- ATPas mediates intracellular acidification required for neurodegeneration in C. elegans. Current Biology, 15: 1249–1254.
21. Yao, H., X. Xiang., L. Chen., A. Guo., F. He., L. Lan., X. Lu. & X. Wu, 2009. Identification of the proteomeof the midgutof silkworm, Bombyx mori L. by multidimension liquid chromatographt LTQ-Orbitrap mass spectrometry. Bioscience reports, 29: 363-373.
22. Yu, M. C., F. Bachand & A. E. McBride, 2004. Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors. Gene, 18: 2024-2035.
23. Zeng, F. & C. A. Cohen, 2000. Partial characterization of α-amylase in the salivary glands of Lygus hesperus and L. linealaris. Comparative Biochemistry and Physiology, 126: 9-16.
24. Zhang, D., M. S. Cupp & E. W. Cupp, 2002. Thrombostatin purification, molecular cloning and expression of novel anti-thrombin protein from horn fly saliva. Insect Biochemistry and Molecular Biology, 32: 321–330.