Molecular characterization of cereal cyst nematodes from South Anatolian Region in Turkey using ITS-rDNA sequences

Abstract

Keywords

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Güney Anadolu Bölgesi Tahıl kist nematodu popülasyonlarının ribozomal DNA'nın ITS bölgesi sekanslanarak moleküler karakterizasyonu

Öz

Tahıl kist nematodlarının (Heterodera avenae group) buğday köklerinde beslenen 12 farklı türü bilinmektedir. Türkiye’de buğday yetiştiriciliğinin yapıldığı farklı alanlarda ekonomik olarak önemli üç tür, Heterodera avenae Wollenweber, 1924, Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984 ve Heterodera latipons Franklin, 1969 bulunmaktadır. Bu çalışmada Güneydoğu Anadolu Bölgesi (Hatay, Kahramanmaraş, Gaziantep, Kilis ve Mardin) buğday alanlarından toplanan 47 adet Tahıl kist nematodu popülasyonuna ait ribozomal DNA’nın transkripte olmayan (ITS-rDNA) bölgesi sekanslanarak moleküler teşhisi yapılmıştır. Filogenetik analiz sonuçlarına göre, H. avenae, H. filipjevi, H. latipons ve H. ciceri Vovlas, Greco & Di Vito, 1985 tanımlanmıştır. Çalışmada kullanılan nematod popülasyonunun % 76,5’i H. latipons, %13’ü H. filipjevi, % 8,5’i H. avenae ve % 2’si Heterodera ciceri olarak saptanmıştır

Anahtar Kelimeler

• Heterodera avenae, Heterodera filipjevi, Heterodera latipons, filogenetik analiz

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