Mitochondrial and ribosomal DNA sequence analysis for discrimination of Trichogramma euproctidis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae)

Abstract

Sequence variation of ribosomal regions, namely the second internal transcribed spacer region of the nuclear ribosomal gene cluster (ITS2), the D2 domain of 28S ribosomal subunit (28SD2), and the mitochondrial cytochrome oxidase I (COI) were examined for differentiation of Trichogramma euproctidis Girault and Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) populations. The length of ITS2 region ranged from 378 bp to 406 bp. The sequences of 28SD2 and COI were 522 bp and 669 bp for T. euproctidis and 530 bp and 672 bp for T. brassicae, respectively. A phylogenetic relationship was constructed by using the maximum likelihood method for each marker. The ITS2 gene region more clearly differentiated between T. euproctidis and T. brassicae than the 28SD2 and COI genes.

Keywords

• Trichogramma euproctidis, Trichogramma brassicae, ITS2, 28SD2, COI

Trichogramma euproctidis Girault ve Trichogramma brassicae Bezdenko (Hymenoptera:Trichogrammatidae)'nin ayırımında mitokondriyal ve ribozomal DNA dizi analizi

Öz

Ribosomal bölgeler dizi varyasyonu: nuclear ribosomal gen dizisinin ikinci genler arası bölgesi (ITS2), 28S ribosomal altünitenin D2 bölgesi (28SD2) ve mitokondrial sitokrom oksidaz I (COI) Trichogramma euproctidis Girault ve Trichogramma brassicae Bezdenko (Hymenoptera: Trichogrammatidae) popülasyonlarının ayırımı için incelenmiştir. ITS2 bölgesinin uzunluğu 378 bç ile 406 bç arasında değişmiştir. 28SD2 ve COI dizilerinin boyutu sırasıyla T. euproctidis için 522 bç ile 669 bç ve T. brassicae için 530 bç ile 672 bç olarak farklı bulunmuştur. Filogenetik ilişki her bir marker için maximum likelihood yöntemi kullanılarak oluşturulmuştur. ITS2 gen bölgesi, T. euproctidis ve T. brassicae’yi 28SD2 ve COI genlerinden daha net ayırt etmiştir.

Anahtar Kelimeler

• Trichogramma euproctidis, Trichogramma brassicae, ITS2, 28SD2, COI

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