Effect of microwave radiation on stored product pest Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvae

Abstract

This study was designed to examine the effects of microwave radiation exposure on Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) larvae. One to two day old larvae were exposed to microwave radiation at the powers of 70, 150, 300 and 600 W for different exposure times (1-50 s). Mortality ratio in larvae increased significantly with increasing exposure time at all powers of microwave radiation. Completele mortality was achieved at the power of 70 W and the longest exposure time (50 s) Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) in the larvae tissue were evaluated.. SOD, CAT, GPx activities decreased and MDA level increased in the microwave radiation-treated larvae tissue compared to control group at 300 and 600 W (P < 0.05). There was no significant DNA damage detectable at 70, 150 and 300 W for 50 s when compared with control group. However, 50 s of 600 W powers was showed effects on tail length and tail intensity indicating DNA damage.These results indicate that high powers of microwave radiation treatments cause some effects on the stored product pest E. kuehniella larvae

Keywords

• Oxidative stress, E. kuehniella larvae, DNA damage, microwave

Depo ürün zararlısı Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) larvaları üzerine mikrodalga radyasyonunun etkisi

Öz

Bu çalışma Ephestia kuehniella (Zeller) (Lepidoptera: Pyralidae) larvaları üzerine mikrodalga radyasyonunun etkilerini araştırmak için düzenlenmiştir. Bir iki günlük larvalar 70, 150, 300 ve 600 W lık mikrodalga radyasyonuna farklı uygulama sürelerinde (1-50 sn) maruz bırakılmıştır. Larvaların ölüm oranı mikrodalga radyasyonun tüm uygulama gücünde artan sürelerle birlikte istatistiksel olarak artmıştır. Tam ölüm 70 W dozunda 50 sn’ye kadar sürerken 600 W’ta ölümlerin başladığı en kısa süre 5 sn’dir. Larva dokularında superoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GPx)’ı ve malondialdehit (MDA) 50 sn için tüm mikrodalga radyasyon gücü için değerlendirilmiştir. 300 ve 600 W gücündeki mikrodalga radyasyonuna maruz kalan larva dokuları ile kontrol grubu karşılaştırıldığında SOD, CAT, GPx aktiviteleri azalırken MDA seviyeleri artmıştır (P < 0.05). 50 sn lik uygulama sonunda 70, 150 ve 300 W ta DNA hasarı bulunmamaktadır. Diğer taraftan DNA hasarını gösteren kuyruk uzunluğu ve kuyruk yoğunluğu kontrol ve uygulama grupları ile karşılaştırıldığında 600 W ta 50 sn deki etkileri gösterilmiştir. Bu sonuçlar gösteriyor ki yüksek mikrodalga radyasyon gücü uygulaması depo ürün zararlısı E. kuehniella larvaları için toksisiteye sebep olmaktadır

Anahtar Kelimeler

• Oksidatif stres, Ephestia kuehniella larvaları, DNA hasarı, mikrodalga

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