Bazı Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) izolatlarının laboratuvar koşullarında kök-ur nematodları üzerine etkileri

Öz

Bu çalışmanın amacı, 2019 yılında Sakarya İli yayla ve meralarından alınan toprak örneklerinden elde edilen entomopatojen fungusların izolasyonunun, teşhisinin yapılması ve Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) olarak teşhisi yapılan beş adet izolatın (S43/1, S43/2, S43/3, S42/1, S42/2), Meloidogyne incognita (Kofoid & White, 1919) ve Meloidogyne javanica (Treub, 1885) (Tylenchida: Meloidogynidae) ile mücadelede kullanım olanaklarının araştırılmasıdır. Denemeler 2020 yılında Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı, Zirai Mücadele Merkez Araştırma Enstitüsü Müdürlüğü Nematoloji Laboratuvarı’nda laboratuvar koşullarında yürütülmüştür. Uygulamadan 24, 48 ve 72 saat sonra ölü larva sayımları yapılmış ve ölüm oranları hesaplanmıştır. S43/1 izolatı M. javanica’nın kontrolünde en etkili izolat olmuş, 108 cfu/ml dozunda 24 saat sonunda %98,5 ve 48 saat sonunda ise %100 ölüme neden olmuştur. S42/2 izolatı M. incognita’ya karşı en etkili izolat olmuş 24 saat sonunda %97.1 ve 72 saat sonunda %100 ölüme neden olmuştur.

Anahtar Kelimeler

• Biyolojik savaş
• Meloidogyne incognita
• Meloidogyne javanica
• Metarhizium anisopliae

Effects of some Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) isolates on root-knot nematodes under laboratory conditions

Abstract

The aim of this study to isolate and diagnose entomopathogenic fungi obtained from soil samples taken from the plateaus and pastures of Sakarya Province in 2019, and to investigate the use of five isolates (S43/1, S43/2, S43/3, S42/1, S42/2) of the diagnosed as Metarhizium anisopliae (Metschn.) Sorokin, 1883 (Hypocreales: Clavicipitaceae) for control Meloidogyne incognita (Kofoid & White, 1919) and Meloidogyne javanica (Treub, 1885) (Tylenchida: Meloidogynidae). The study was conducted at the Laboratory of Nematology in Plant Protection Central Research Institute (Republic of Turkey Ministry of Agriculture and Forestry) in 2020. The experiments were performed under laboratory conditions. Counts of dead larvae were made 24, 48 and 72 h post application and mortality rates calculated. Isolate S43/1 was the most effective isolate for control M. javanica at 108 cfu/ml causing 98.5% mortality after 24 h and 100% after 48 h. Isolate S42/2 was the most effective against M. incognita at 108 cfu/ml and with 97.1% mortality after 24 h and 100% after 72 h.

Keywords

• Biocontrol
• Meloidogyne incognita
• Meloidogyne javanica
• Metarhizium anisopliae

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