Bioactivity of a betabaculovirus, Hyphantria cunea granulovirus, in six lepidopteran insects as potential hosts

Year 2021, , 417 - 424, 15.12.2021

Zeynep Bayramoğlu , Dönüş Gençer , İsmail Demir

https://doi.org/10.16970/entoted.974579

Cited By: 1

Abstract

The aim of this study, conducted in 2018 and 2020, was to investigate the bioactivity of a local baculovirus isolate, Hyphantria cunea granulovirus (HycuGV), in seven lepidopteran pests. Based on data collected 10 days after exposure, HycuGV was found to infect Malacosoma neustria (L., 1758) (Lepidoptera: Lasiocampidae), Lymantria dispar (L., 1758) (Lepidoptera: Erebidae), Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) and Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae) larvae as well as its host Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae). However, it did not infect Spodoptera littoralis (Boisduval, 1883) (Lepidoptera: Noctuidae) and Cydia pomonella (L., 1758) (Lepidoptera: Tortricidae). A HycuGV dose rate experiment indicated LC50 of 4.7x105 occlusion bodies (OBs)/ml in H. cunea, 5.6x106 OBs/ml in L. dispar, 7x107 OBs/ml in S. exigua, 1.5x109 OBs/ml in M. neustria and 7.7x109 OBs/ml in H. armigera. HycuGV was infectious to S. exigua and L. dispar, but only provided effective control in M. neustria and H. armigera at high dose rates. These findings demonstrate that HycuGV can be highly effective for control of S. exigua, L. dispar and H. cunea.

Keywords

Baculovirus, bioactivity, biological control, host range, Hyphantria cunea granulovirus

Supporting Institution

Karadeniz Teknik Üniversitesi

Project Number

KTU BAP 5173

Thanks

This study was supported by the Karadeniz Technical University Research Foundation (grant number KTU BAP 5173).

Betabaculovirüs, Hyphantria cunea granulovirüs’ün potansiyel konukçu olarak altı lepidopter böcekteki biyoaktivitesi

Abstract

2018 ve 2020 yıllarında yürütülen bu çalışmanın amacı, yerel bir bakülovirüs izolatı Hyphantria cunea granulovirus (HycuGV)'ün yedi lepidopter zararlısı üzerindeki biyoaktivitesinin araştırılmasıdır. Denemeden sonraki 10. günde elde edilen verilere göre HycuGV’nin kendi konukçusu olan Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae)’nın yanı sıra Malacosoma neustria (L., 1758) (Lepidoptera: Lasiocampidae), Lymantria dispar (L., 1758) (Lepidoptera: Erebidae), Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae) ve Spodoptera exigua (Hübner, 1808) (Lepidoptera: Noctuidae) larvaları üzerinde de enfeksiyon oluşturma kabiliyetine sahip olduğu belirlenirken, Spodoptera littoralis (Boisduval, 1883) (Lepidoptera: Noctuidae) ve Cydia pomonella (L., 1758) (Lepidoptera: Tortricidae) larvalarında enfeksiyon oluşturmadığı tespit edildi. HycuGV’nin doz denemelerinde LC50 değeri, H. cunea’da 4.7x105 OBs/ml, L. dispar’da 5.6x106 OBs/ml, S. exigua’da 7x107 OBs/ml, M. neustria’da 1.5x109 OBs/ml ve H. armigera’da 7.7x109 OBs/ml olarak hesaplandı. Bu sonuçlar HycuGV’nin S. exigua ve L. dispar için bulaşıcı olduğunu, ancak M. neustria ve H. armigera’da ise yüksek doz oranlarında etkili olduğunu gösterdi. Bu bulgular, HycuGV’nin S. exigua, L. dispar ve H. cunea’nın mücadelesi için oldukça etkili olabileceğini göstermektedir.

Keywords

Bakülovirüs, biyoaktivite, biyolojik mücadele, konukçu aralığı, Hyphantria cunea granulovirus

Project Number

KTU BAP 5173

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