Response of tomato seedlings with different number of true leavesto Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949

Year 2016, Volume: 40 Issue: 4, 377 - 383, 13.12.2016

Zübeyir Devran , İbrahim Mıstanoğlu Tevfik Özalp

Abstract

Tomato is one of the most important vegetables grown throughout the world. Root-knot nematodes cause significant economic yield losses in tomato. Development of tomato cultivars which are resistant to root-knot nematodes is the most effective management method. In tomato, resistance to root-knot nematodes is determined by bioassay and molecular markers. Molecular markers are commonly used to screen for resistance genes in breeding programs. However, bioassays are also required for identification of the performance of resistance genes. Different parameters such as stage of seedling, soil temperature, nematode quantity and nematode virulence effect performance of bioassays. In the present study, the response of tomato seedlings with different numbers of true leaves to Meloidogyne incognita isolate S6 was compared under controlled conditions. Seedlings showed different reactions to M. incognita inoculation. The results indicated that stage of tomato seedlings can be important for bioassay and that tomato seedlings with four true leaves are best for nematode testing. These results will help in the optimization of root-knot nematode tests used in tomato breeding.

Keywords

Bioassay, Meloidogyne, tomato seedling, reaction

Farklı sayıda gerçek yapraklı döneme sahip domates fidelerinin Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949’ya tepkileri

Abstract

Domates dünyada yetiştiriciliği yapılan en önemli sebzelerden birisidir. Kök-ur nematodları domateste önemli düzeyde ekonomik kayıplara neden olmaktadır. Kök-ur nematodlarına dayanıklı domates çeşitlerinin geliştirilmesi en önemli mücadele yöntemidir. Domateste kök-ur nematodlarına dayanıklılık biyolojik testler ve moleküler markörler tarafından belirlenebilmektedir. Moleküler markörler ıslah programlarında dayanıklılık geninin tespitinde yaygın şekilde kullanılmaktadır. Bununla birlikte dayanıklılık geninin performansının belirlenmesi için biyolojik testler gereklidir. Nematod virülensliği, nematod sayısı, toprak sıcaklığı ve fidenin dönemi gibi farklı parametreler biyolojik testin performansını etkilemektedir. Bu çalışmada, farklı sayıda gerçek yaprak dönemine sahip domates fidelerinin M. incognita’ nın S6 izolatına tepkisi kontrollü koşullar altında karşılaştırılmıştır. Fideler M. incognita inokulasyonuna farklı farklı tepkiler göstermiştir. Sonuçlar, biyolojik test için domates fidesinin döneminin önemli olduğunu ve nematod testi için dördüncü gerçek yapraklı döneme sahip fidelerin en uygun olduğunu göstermiştir. Bu sonuçlar, domates ıslahında kullanılacak olan kök-ur nematodlar testlerinin optimizasyonuna yardım edecektir.

Keywords

Biyolojik test, Meloidogyne, domates fidesi, reaksiyon

References

• Abad, P., B. Favery, M. N. Rosso & P. Castagnone-Sereno, 2003. Root-knot nematode parasitism and host response: Molecular basis of a sophisticated interaction. Molecular Plant Pathology, 4 (4): 217–224.
• Bird, A. F., 1967. Changes associated with parasitism in nematodes. I. Morphology and physiology of preparasitic and parasitic larvae of Meloidogyne javanica. The Journal of Parasitology, 768-776.
• Devran, Z. & M. A. Söğüt, 2009. Distribution and identification of root-knot nematodes from Turkey. Journal of Nematology, 41: 128-133.
• Devran, Z. & M. A. Söğüt, 2011. Characterizing races of Meloidogyne incognita, M. javanica and M. arenaria in the West Mediterranean region of Turkey. Crop Protection, 30: 451-455.
• Devran, Z., B. Başköylü, A. Taner & F. Doğan, 2013. Comparison of PCR-based molecular markers for identification of Mi gene. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science, 45: 395-402.
• Devran, Z. & M. A. Söğüt, 2014. Response of heat-stable tomato genotypes to Mi-1 virulent root-knot nematode populations. Turkish Journal of Entomology, 38: 229-238.
• Hooper D. J., 1986. “Handling, Fixing, Staining and Mounting Nematodes, 58-80”. In: Laboratory Methods for Work with Plant and Soil Nematodes, (Eds: J.F. Southey) Her Majesty’s Stationery Office, London. 202 pp.
• Huang, C. S., 1985. “Formation, Anatomy and Physiology of Giant Cells Induced by Root-Knot Nematodes, 155-164”. In: An Advanced Treatise on Meloidogyne, Vol. 1, (Eds: J. N. Sasser & C. C. Carter), Raleigh: North Carolina State University Graphics, 223 pp.
• Hussey, R. S. & C. W. Mims., 1990. Ultrastructure of esophageal glands and their secretory granules in root-knot nematode Meloidogyne incognita. Protoplasma, 156: 9-18.
• Karssen, G. & M. Moens., 2006. “Root-Knot Nematodes. 59-90” In: Plant Nematology, (Eds: R. N. Perry & M. Moens), CABI, London, 447 pp.
• Khan, H., R. Ahmad, A. S. Akhtar, A. Mahmood, T. Basit, & T. Niaz, 2000. Effect of inoculum density of Meloidogyne incognita and plant age on the severity of root-knot disease in tomato. International Journal of Agriculture & Biology, 2000 (02): 360–363.
• Melillo, M. T., P. Leonetti, M. Bongiovanni, P. Castagnone‐Sereno & T. Bleve‐Zacheo, 2006. Modulation of reactive oxygen species activities and H2O2 accumulation during compatible and incompatible tomato–root‐knot nematode interactions. New Phytologist, 170 (3): 501-512.
• Ramsay, K., Z. Wang & M. G. K. Jones, 2004. Using laser capture microdissection to study gene expression in early stages of giant cells induced by Root‐Knot Nematodes. Molecular Plant Pathology, 5 (6): 587-592.
• Schomaker, C. H. & T. H. Been, 2006. “Plant Growth and Population Dynamics, 275-301”. In: Plant Nematology, (Eds: R.N. Perry & M. Moens), British Library, London, UK, 447 pp.
• Seah, S., V. M. Williamson, B. E. Garcia, L. Mejía, M. S. Salus, C. T. Martin & D. P. Maxwell, 2007. Evaluation of a co-dominant SCAR marker for detection of the Mi-1 locus for resistance to root-knot nematode in tomato germ plasm. Tomato Genetic Cooperative Report, 57: 37–40.
• Shane, W. W. & K. R. Barker, 1986. Effects of temperature, plant age, soil texture, and Meloidogyne incognita on early growth of soybean. Journal of Nematology, 18 (3): 320-327.
• Wesemael, W. M. L., N. Viaene & M. Moens, 2006. The influence of root diffusate and host age on hatching of the root-knot nematodes, Meloidogyne chitwoodi and M. fallax. Nematology, 8 (6): 895-902.
• Wesemael, W. M. L., R. N. Perry & M. Moens, 2011. Root-knot nematodes (Meloidogyne spp.) in Europe. Nematology, 13 (1): 3-16.
• Williamson, V. M., 1998. Root-knot nematode resistance genes in tomato and their potential for future use. Annual Review of Phytopathology, 36: 277-293
• Williamson, V. M., 1999. Plant nematode resistance genes. Plant Biology, 2: 327–331.