Çukurova Bölgesi (Türkiye) pamuk alanlarında Aphis gossypii (Glover) (Hemiptera: Aphididae) neonikotinoid direnci

Year 2018, Volume: 42 Issue: 1, 23 - 31, 20.04.2018

Selçuk Ulusoy , Ekrem Atakan , Sadık Dinçer

https://doi.org/10.16970/entoted.380010

Cited By: 8

Abstract

Pamuk yaprakbiti, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), pamuk tarım alanlarında ana
zararlılardandır. Neonicotinoidler, Akdeniz Bölgesi'nde A. gossypii ve diğer herbivor böceklerin mücadelesinde oldukça yaygın
kullanılan önemli bir insektisit grubudur. Ancak, Çukurova Bölgesi'nde A. gossypii'ye karşı bazı insektisit uygulamaları yüksek oranlarda
kullanılmasına rağmen başarısız olmuştur. Bu nedenle, bu bölgedeki pamuk
alanlardan 2015-2016 toplanan A. gossypii popülasyonlarında
imidacloprid ve thiamethoxam dayanıklılık düzeyi belirlemek amacıyla biyoassay ve enzim analizleri
yapılmıştır. Analizler sonucunda imidacloprid için 54.6-206.5 (dirençlilik
faktörü: RF) arasında, thiamethoxam için 5.7-65.7 arasında LD50 dayanıklılık katsayıları
bulunmuştur. Kürkçüler (RF 206.55) popülasyonu imidacloprid için, Körkuyu (RF
65.72) popülasyonu da thiamethoxam için en yüksek LD50 değerine sahiptir. Enzim
analizi istatistiki anlamda yüksek metabolik direnci ortaya çıkarmıştır. Her
iki insektisit içinde en yüksek enzim akitviteleri, karboksil esteraz enzimi
Körkuyu popülasyonunda 17,8 nM/dk/mg protein, glutatyon S-transferaz (GSTs)
enzimi Bahçe popülasyonunda 142,3 nM/dk/mg protein ve Körkuyu popülasyonunda
3,8 nM/dk/mg protein ile en yüksek monooksigenaz P450 enzim aktivitesi
bulunmuştur. Bu çalışma, Türkiye’de neonicotinoidlere karşı A.
gosyypii’de direnç gelişmesini ve bu direncin kırılması için yeni yönetim
stratejilerine ihtiyaç olduğunu ortaya koymuştur.

Keywords

Aphis gossypii, biyoassay, pamuk, neonicotinoid, dirençlilik

Neonicotinoid resistance of Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) in cotton fields of Çukurova Region, Turkey

Abstract

Cotton
aphid, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), is a major
pest in cotton fields. Neonicotinoids are important and highly prevalent
insecticides currently used against A. gossypii and other herbivorous
insect pests in the Mediterranean Region of Turkey. However, some insecticide
applications against A. gossypii in the Çukurova Region have failed
despite using high rates. Therefore, bioassays and enzyme analyses were
conducted to determine resistance to imidacloprid and thiamethoxam in A.
gossypii populations collected in 2015-2016 from cotton fields in this
region. Resistance factors (RF) were 54.6 to 206.5 fold for imidacloprid and
5.7 to 65.7 fold for thiamethoxam. Populations from Kürkçüler (RF 206.5) had
the highest LD50 for imidacloprid and from Körkuyu (RF 65.7) for
thiamethoxam. Enzyme analysis revealed statistically higher metabolic
resistance. Maximum enzyme activities were 17.8, 142.3 and 3.8 nM/min/mg
protein for carboxylesterase for in Körkuyu, for glutathione S-transferase in
Bahçe and for cytochrome P450 monooxygenase in Körkuyu, respectively. This
study revealed the development of resistance in A.gossypii to
neonicotinoid insecticides in Turkey and the need for new management strategies
to break this resistance.

Keywords

Aphis gossypii, biyoassay, cotton, neonicotinoid, resistance

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